1887

Abstract

The restriction enzyme profiles of 16S ribosomal DNAs (rDNAs) amplified by PCR from thermophilic heterotrophic bacterial strains isolated from composts were compared with those of reference strains. This allowed us to assign all but 1 of 16 strains to four different species (namely, , and “”). This study showed that PCR restriction analysis of 16S rDNA contributes to rapid and reliable identification of newly isolated strains belonging to recognized species.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-47-4-1246
1997-10-01
2024-04-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/47/4/ijs-47-4-1246.html?itemId=/content/journal/ijsem/10.1099/00207713-47-4-1246&mimeType=html&fmt=ahah

References

  1. Aragno M. 1992 Thermophilic, aerobic, hydrogen-oxidizing (Knallgas) bacteria. 3917–3933 Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K.-H.ed The prokaryotes, 2nd. Springer-Verlag; New York, N.Y.:
    [Google Scholar]
  2. Ash C., Farrow J. A. E., Wallbanks S., Collins M. D. 1991; Phylogenetic heterogeneity of the genus Bacillus revealed by comparative analysis of small-subunit-ribosomal RNA sequences. Lett. Appl. Microbiol. 13:202–206
    [Google Scholar]
  3. Beffa T., Blanc M., Aragno M. 1996; Obligately and facultatively autotrophic, sulfur- and hydrogen-oxidizing thermophilic bacteria isolated from hot composts. Arch. Microbiol. 165:34–40
    [Google Scholar]
  4. Beffa T., Blanc M., Lyon P.-F., Vogt G., Marchiani M., Lott Fischer J., Aragno M. 1996; Isolation of Thermus strains from hot composts (60 to 80°C). Appl. Environ. Microbiol. 62:1723–1727
    [Google Scholar]
  5. Daron H. H. 1973; Nutritional alteration of the fatty acid composition of a thermophilic Bacillus species. J. Bacteriol. 116:1096–1099
    [Google Scholar]
  6. Donk P. J. 1920; A highly resistant thermophilic organism. J. Bacteriol. 5:373–374
    [Google Scholar]
  7. Fujio Y., Kume S. J. 1991; Isolation and identification of thermophilic bacteria from sewage sludge compost. J. Ferment. Bioeng. 72:334–337
    [Google Scholar]
  8. Gordon R. E., Haynes W. C., Hor-Nay Pang C. 1973 The genus Bacillus. Agricultural handbook no. 427 Agricultural Research Service, U.S. Department of Agriculture; Washington, D.C.:
    [Google Scholar]
  9. Klaushofer H., Hollaus F. 1970; Zur Taxonomie der Hochthermophilen, in Zuckerfabrikssaften vorkommenden aeroben Sporenbildner. Z. Zuckerind. 20:465–470
    [Google Scholar]
  10. Logan N. A., Berkeley R. C. W. 1981 Classification and identification of members of the genus Bacillus using API tests. 105–140 Berkeley R. C. W., Goodfellow M.ed The aerobic endospore-forming bacteria Academic Press, Ltd.; London, United Kingdom.:
    [Google Scholar]
  11. Logan N. A., Berkeley R. C. W. 1984; Identification of Bacillus strains using the API system. J. Gen. Microbiol. 130:1871–1882
    [Google Scholar]
  12. Manachini P. L., Craveri A., Guicciardi A. 1968; Composizione in basidell’acido desossiribonucleico di forme mesofile termofacoltative e termofile dell’genere Bacillus. Ann. Microbiol. Enzimol. 18:1
    [Google Scholar]
  13. Pitcher D. G., Saunders N. A., Owen R. J. 1989; Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett. Appl. Microbiol. 8:151–156
    [Google Scholar]
  14. Rainey F. A., Fritze D., Stackebrandt E. 1994; The phylogenetic diversity of thermophilic members of the genus Bacillus as revealed by 16S rDNA analysis. FEMS Microbiol. Lett 115:205–212
    [Google Scholar]
  15. Scholz T., Demharter W., Henzel R., Kandler O. 1987; Bacilluspallidus sp. nov., a new thermophilic species from sewage sludge. Syst. Appl. Microbiol. 9:91–96
    [Google Scholar]
  16. Sharp R. J., Riley P. W., White D. 1991 Heterotrophic thermophilic bacteria. 19–50 Kristjansson J. K.ed Thermophilic bacteria CRC Press, Inc.; Boca Raton, Fla.:
    [Google Scholar]
  17. Smibert R. M., Krieg N. R. 1994 Phenotypic characterization. 607–654 Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R.ed Methods for general and molecular bacteriology American Society for Microbiology; Washington, D.C.:
    [Google Scholar]
  18. Stalder V., Marchiani M., Aragno M., Bachofen R. 1994; Characterization and identification of a new strain of esterase-producing Bacillus thermoglucosidasius “EAEC” from an aerated thermophilic sewage sludge. Microbiol. Res. 149:1–7
    [Google Scholar]
  19. Strom P. F. 1985; Effect of temperature on bacterial species diversity in thermophilic solid-waste composting. Appl. Environ. Microbiol. 50:899–905
    [Google Scholar]
  20. Strom P. F. 1985; Identification of thermophilic bacteria in solid-waste composting. Appl. Environ. Microbiol. 50:906–913
    [Google Scholar]
  21. Suzuki Y., Kishigami T., Inoue K., Mizoguchi Y., Eto N., Takagi M., Abe S. 1983; Bacillus thermoglucosidasius sp. nov., a new species of obligately thermophilic bacilli. Syst. Appl. Microbiol. 4:487–495
    [Google Scholar]
  22. White D., Sharp R. J., Priest F. G. 1993; A polyphasic taxonomic study of thermophilic bacilli from a wide geographical area. Antonie van Leeuwenhoek 64:357–386
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-47-4-1246
Loading
/content/journal/ijsem/10.1099/00207713-47-4-1246
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error