1887

Abstract

Three strains of creatinine-hydrolysing bacteria isolated from human urine were characterized taxonomically. They were aerobic, non-spore-forming, Gram-positive rods with the peptidoglycan of the cell wall containing lysine. MK-8 and MK-9 were found to be the major types of menaquinone. The G+C content of the DNA was 66–67 mol%. The 16S rRNA sequence of one strain (GIFU 12498) was determined and aligned with other high-G+C-content Grampositive rods from different genera. Following phylogenetic analysis, this strain was placed in the genus was the most closely related species in the phylogenetic tree, and this species also showed the highest sequence homology value (97%) with GIFU 12498. However, DNA-DNA hybridization indicated that GIFU 12498 did not belong to (33·8±13·5% chromosomal similarity). The three urine strains belonged to one species because they shared more than 95% DNA-DNA similarity. It is proposed that these strains are placed in the genus as a new species, sp. nov. The type strain of is GIFU 12498, which has been deposited in the Japan Collection of Microorganisms (JCM) with the accession number JCM 10102.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-48-2-423
1998-04-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/48/2/ijsem-48-2-423.html?itemId=/content/journal/ijsem/10.1099/00207713-48-2-423&mimeType=html&fmt=ahah

References

  1. American Type Culture Collection 1992 Catalogue of Bacteria and Bacteriophages, 18th. Rockville, MD: American Type Culture Collection;
    [Google Scholar]
  2. Briglia M., Rainey F. A., Stackebrandt E., Schraa D., Salkinoja-Salonen M. S. 1996; Rhodococcus percolatus sp. nov., a bacterium degrading 2,4,6-trichlorophenol. Int J Syst Bacterial 46:23–30
    [Google Scholar]
  3. Brimacombe R., Geruer B., Mitchell P., Osswald M., Rinke-Appel J., Schuler D., Stade K. 1990; Three dimensional structure and function of Escherichia coli 16S and 23S rRNA as studied by cross-linking techniques. The Ribosome93–106 Edited by Water E. H., Albert D., Roger A. G., Moor B. M., David S., Jonathan R. W. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  4. Collins M. D., Jones D. 1981; The distribution of isoprenoid quinone structural types in bacteria and their taxonomic implications. Microbiol Rev 45:316–154
    [Google Scholar]
  5. Collins M. D., Jones D., Keddie R. M., Kroppenstedt R. M., Scheifer K. H. 1983; Classification of some coryneform bacteria in a new genus Aureobacterium. Syst Appl Microbiol 4:236–252
    [Google Scholar]
  6. Collins M. D., Kroppenstedt R. M. 1983; Lipid composition as a guide to the classification of some coryneform bacteria-containing A4a type peptidoglycan (Schleifer and Kandler). Syst Appl Microbiol 4:95–104
    [Google Scholar]
  7. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230
    [Google Scholar]
  8. Conn H. J., Dimmick I. 1947; Soil bacteria similar in morphology to Mycobacterium and Corynebacterium. J Bacteriol 54:291–303
    [Google Scholar]
  9. Cummins C. S., Harris H. 1959; Taxonomic position of Arthrobacter. Nature 184:831–832
    [Google Scholar]
  10. Ezaki T., Li N., Hashimoto Y., Miura H., Yamamoto H. 1994; 16S ribosomal DNA sequences of anaerobic cocci and proposal of Ruminococcus hansenii comb. nov. and Ruminococcus productus comb. nov. Int J Syst Bacteriol 44:130–136
    [Google Scholar]
  11. Ezaki T., Saidi S. M., Liu S.-L., Hashimoto Y., Yamamoto H., Yabuuchi E. 1990; Rapid procedure to determine the DNA base composition from small amounts of Grampositive bacteria. FEMS Microbiol Lett 67:127–130
    [Google Scholar]
  12. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229
    [Google Scholar]
  13. Ezaki T., Hashimoto Y., Takauchi N., Yamamoto H., Liu S., Miura H., Matsui K., Yabuuchi E. 1988; Simple genetic methods to identify viridans group streptococci by colorimetric dot hybridization and fluorometric hybridization in microdilution wells. J Clin Microbiol 26:1708–1713
    [Google Scholar]
  14. Goto K., Tanaka H., Imai J., Kawai M. 1989; Bacteria contamination of the infant urine samples from filter papers used in neuroblastoma-screening tests. Jpn J Public Health 36:645–651
    [Google Scholar]
  15. Goto K., Nagano I., Tokoro M., Imai J., Iwada H., Kawai M. 1990; The study of bacterial contamination of filtered urine in neuroblastoma screening. Rinsho Biseibutsu 17:83–88
    [Google Scholar]
  16. Jones D., Keddie R. M. 1992; Arthrobacter. The Prokaryotes, 2nd.1283–1299 Edited by Balows A., Triiper H. G., Dworkin M., Harder W., Schleifer K.-H. New York: Springer;
    [Google Scholar]
  17. Kawamura Y., Hou X. G., Sultana F., Liu S. J., Yamamoto H., Ezaki T. 1995; Transfer of Streptococcus adjacens and Streptococcus defectivus to Abiotrophia gen. nov. as Abiotrophia adiacens comb. nov. and Abiotrophia defectiva comb, nov., respectively. Int J Sy st Bacteriol 45:798–803
    [Google Scholar]
  18. Keddie R. M., Collins M. D., Jones D. 1986; Genus Arthrobacter Conn and Dmmik. Bergey’s Manual of Systematic Bacteriology 21288–1303 Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  19. Koch C., Rainey F. A., Stackebrandt E. 1994; 16S rDNA studies on members of Arthrobacter and Micrococcus: an aid for their future taxonomic restructuring. FEMS Microbiol Lett 123:167–172
    [Google Scholar]
  20. Koch C., Schumann P., Stackebrandt E. 1995; Reclassification of Micrococcus agilis (Ali-Cohen 1889) to the genus Arthrobacter as Arthrobacter agilis comb. nov. and emendation of genus Arthrobacter. Int J Syst Bacteriol 45:837–839
    [Google Scholar]
  21. Kusunoki S., Ezaki T. 1992; Proposal of Mycobacterium peregrinum sp. nov., nom. rev., and elevation of Mycobacterium chelonae subsp. abscessus (Kubica et al.) to species status: Mycobacterium abscessus comb. nov. Int J Syst Bacteriol 42:240–245
    [Google Scholar]
  22. Nash P., Krenz M. M. 1991; Culture media. Manual of Clinical Microbiology, 5th.1226–1314 Edited by Balows A., Hausler W. J. Jr, Herrmann K. L., Isenberg H. D., Shadomy H. J. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  23. Schubert K., Ludwig W., Springer N., Kroppenstedt N. M., Accolas J. P., Fiedler F. 1996; Two coryneform bacteria isolated from the surface of French gruyere and beaufort cheese are new species of genus Brachybacterium: Brachybacterium alimentarium sp. nov. and Brachybacterium tyrofermentans sp. nov. Int J Syst Bacteriol 46:81–87
    [Google Scholar]
  24. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849
    [Google Scholar]
  25. Stackebrandt E., Fowler V. J., Fiedler F., Seiler H. 1983; Taxonomic studies on Arthrobacter nicotianae and related taxa: description of Arthrobacter uratoxydans sp. nov. and Arthrobacter sulfureus sp. nov. and reclassification of Brevibacterium protophormiae as Arthrobacter protophormiae comb. nov. Syst Appl Microbiol 4:470–486
    [Google Scholar]
  26. Stahl D. A., Urbance J. W. 1990; The division between fast- and slow-growing species corresponds to natural relationships among the mycobacteria. J Bacteriol 172:116–124
    [Google Scholar]
  27. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequencing weighing, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680
    [Google Scholar]
  28. Williams A., Collins M. D. 1995; Reclassification of Oribacterium catoniae (Moore and Moore 1994) as Porphyromonas catoniae comb. nov. and emendation of the genus Porphyromonas. Int J Syst Bacteriol 45:578–581
    [Google Scholar]
  29. Yano I., Yamada Y., Suzuki K. I. 1987; Analyses of bacterial ingredients. Bacterial Identification in Accordance with Recent Taxonomy: Use of Phenotypic, Chemical, and Genetic Analysis41–85 Edited by Yabuuchi E., Ezaki T., Park Y. H., Sugawara H., Suzuki K. I., Yamada Y., Yamanoto H., Yano I. Tokyo: Saikon Publications;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-48-2-423
Loading
/content/journal/ijsem/10.1099/00207713-48-2-423
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error