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Volume 49, Issue 1

New genus-specific primers for the PCR identification of members of the genera and Free

Abstract

Members of the family are difficult to identify on the basis of their micromorphology only. The biochemical characterization of each new isolate is a painstaking and time-consuming task which cannot always be undertaken when handling large numbers of strains as is the case in natural product screening programmes. In this study, two sets of genus-specific oligonucleotides were designed which allow rapid detection of members of the genera and by means of PCR-specif ic amplification. The genus specificity of these primers was validated on a wide range of collection strains and the primers were subsequently used to study a group of 106 wild-type isolates that possessed morphological characteristics of the family. Out of this group, 51 strains could be identified as members of the genus and only nine isolates could be assigned to the genus The diversity indicated by whole-cell fatty acid profiles of both wild-type and reference strains was compared with that identified using the oligonucleotide primers. The partial 16S rDNA sequencing of representative wild-type strains was used to validate their genus assignment by PCR-specif ic amplification. This study shows the industrial usefulness of the application of these direct identification tools as well as the complementary use of two sources of data, PCR-specif ic amplification results and fatty acid composition, to assess the diversity of a microbial population.

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Keyword(s): 16S rDNA sequences , PCR primers and Pseudonocardiaceae
© 1999, International Union of Microbiological Societies
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1999-01-01
2024-04-23
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New genus-specific primers for the PCR identification of members of the genera Pseudonocardia and Saccharopolyspora
Int J Syst Evol Microbiol 49, 149 (1999); https://doi.org/10.1099/00207713-49-1-149
/content/journal/ijsem/10.1099/00207713-49-1-149
/content/journal/ijsem/10.1099/00207713-49-1-149
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