Sulfitobacter mediterraneus sp. nov., a new sulfite-oxidizing member of the α-Proteobacteria
Pukall, Rüdiger and Buntefuß, Daniela and Frühling, Anja and Rohde, Manfred and Kroppenstedt, Reiner M. and Burghardt, Jutta and Lebaron, Philippe and Bernard, Laetitia and Stackebrandt, Erko,, 49, 513-519 (1999),
doi = https://doi.org/10.1099/00207713-49-2-513,
publicationName = Microbiology Society,
issn = 1466-5026,
abstract= Analysis of PCR products of 16S rDNA of 680 isolates from Mediterranean Sea mesocosm experiments with taxon-specific 16S rDNA oligonucleotides revealed that 262 isolates belonged to the α subclass of the class Proteobacteria. Partial 16S rDNA sequence analysis of selected isolates and oligonucleotide probing with a Sulfitobacter-specific 16S rDNA probe affiliated 33 strains to the genus Sulfitobacter. Analysis of the Haelll digest pattern of 16S rDNA revealed the presence of two groups; while 30 strains showed a pattern identical with that obtained for Sulfitobacter pontiacus DSM 10014T, a second group of three strains had a unique pattern that was different from that of the type strain. Five isolates of group 1 and one isolates of group 2, strain CH-B427T, were selected for detailed taxonomic analysis. All six isolates closely resembled the type strain Sulfitobacter pontiacus DSM 10014Tin physiological reactions. However, strain CH-B427Tdiffered quantitatively in the composition of fatty acids from Sulfitobacter pontiacus DSM 10014Tand showed only 98·2% 16S rDNA sequence similarity with strain DSM 10014T. DNA-DNA reassociation value obtained for strains DSM 10014Tand CH-B427Trevealed 46 % similarity. Based on the results of DNA-DNA reassociation and discrete differences in the nucleotide composition of 16S rDNA, a new species of the genus Sulfitobacter is proposed, designated Sulfitobacter mediterraneus sp. nov., the type strain being strain CH-B427T(= DSM 12244T).,
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