RT Journal Article SR Electronic(1) A1 Jeanthon, Christian A1 L’Haridon, Stéphane A1 Pradel, Nathalie A1 Prieur, DanielYR 1999 T1 Rapid identification of hyperthermophilic methanococci isolated from deep-sea hydrothermal vents JF International Journal of Systematic and Evolutionary Microbiology, VO 49 IS 2 SP 591 OP 594 DO https://doi.org/10.1099/00207713-49-2-591 PB Microbiology Society, SN 1466-5034, AB 16S rDNAs amplified by PCR from 22 hyperthermophilic methanococci isolated from deep-sea hydrothermal vents were compared with those of the six type strains of the genus Methanococcus by RFLP analysis. Restriction fragments obtained with Haelll enabled four of the type species to be distinguished. Restrictions with HhaI, BstUI and MspI were necessary to differentiate Methanococcus jannaschii and Methanococcus fervens. The results indicate that the 16S rDNA PCR-RFLP method provides a rapid and reliable tool for the identification of newly isolated hyperthermophilic Methanococcus spp., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-49-2-591