1887

Abstract

The taxonomic status of an actinomycete (MA140/96) isolated from indoor building materials of a children’s day care centre was studied using the polyphasic approach. The cell morphology was atypical for an actinomycete, electron microscopy revealed a hairy surface, highly unusual for Gram-positive bacteria. The organisms grew at 10–37 °C, no growth was visible at 5 °C and 45 °C in 5 d. The cell wall contained the diamino acid meso-diaminopimelic acid and the sugars arabinose, galactose, mannose and ribose. The phospholipids phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol and diphosphatidylglycerol were detected. The only menaquinone found was MK-9(H). The fatty acid pattern was composed of palmitic acid (23·6%) palmitoleic acid (16·5%) and another hexadecenoic acid 16:111 (1·4%), oleic acid (29·9%), stearic acid (2·9%) and the 10-methyl-branched tuberculostearic acid (23·2%). A gas-chromatographic analysis of the mycolic acid revealed a carbon-chain length of C–C. The G+C was 64·8 mol%. The results of 16S rDNA sequence comparisons revealed that strain MA140/96 represents a new lineage in the suborder of the order . Therefore, it was concluded that strain MA140/96 should be assigned to a new genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain of the species is MA140/96 (= DSM 44343).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-49-2-681
1999-04-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/49/2/ijs-49-2-681.html?itemId=/content/journal/ijsem/10.1099/00207713-49-2-681&mimeType=html&fmt=ahah

References

  1. Andersson M., Laukkanen M., Nurmiaho-Lassila E.-L., Rainey F., Niemelä S., Salkinoja-Salonen M. 1995; Bacillus thermosphaericus sp. nov a new thermophilic ureolytic Bacillus isolated from air. Syst Appl Microbiol 18:203–220
    [Google Scholar]
  2. Andersson M. A., Nikulin M., Koljalg U., Andersson M. C., Rainey F., Reijula K., Hintikka E.-L., Salkinoja-Salonen M. 1997; Bacteria, molds, and toxins in water-damaged building materials. Appl Environ Microbiol 63:387–393
    [Google Scholar]
  3. Briglia M., Rainey F. A., Stackebrandt E., Schraa G., Salkinoja-Salonen M. 1996; Rhodococcuspercolatus sp. nov., a bacterium degrading 2,4,6 tetrachlorophenol. Int J Syst Bacteriol46
    [Google Scholar]
  4. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. 1978; Complete nucleotide sequence of the 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad SciUSA 75:4801–4805
    [Google Scholar]
  5. Collins M. D., Jones D. 1981; Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implications. Microbiol Rev 45:316–354
    [Google Scholar]
  6. De Soete G. 1983; A least squares algorithm for fitting additive trees to proximity data. Psychometrika 48:621–626
    [Google Scholar]
  7. DSMZ 1998 Catalogue of Strains, 5th. German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany: Deutsche Sammlung von Mikroorganismen und Zellkulturen;
    [Google Scholar]
  8. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. 1994 Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology;
    [Google Scholar]
  9. Goodfellow M. 1989; The actinomycetes. I. Suprageneric classification of actinomycetes. In Bergey’s Manual of Systematic Bacteriology 42333–2339 Williams S. T., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  10. Groth I., Schumann P., Rainey F. A., Martin K., Schuetze B., Augsten K. 1997; Bogoriella caseolytica gen. nov. sp. nov., a new alkaliphilic actinomycete from a soda lake in Africa. Int J Syst Bacteriol 47:788–794
    [Google Scholar]
  11. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism 321–132 Munro H. N. New York: Academic Press;
    [Google Scholar]
  12. Kämpfer P., Denner E. B. M., Meyer S., Moore E. R. B., Busse H.-J. 1997; Classification of “Pseudomonas azotocolligans” Anderson 1955, 132, in the genus Sphingomonas as Sphingomonas trueperi sp. nov. Int J Syst Bacteriol 47:577–583
    [Google Scholar]
  13. Kiatte S. 1994 Polyphasische automatisierte Identifizierung der Gattungen Corynebacterium Lehmann & Neumann 1896 und Rhodococcus Zopf 1891. PhD thesis University of Osnabruck;
    [Google Scholar]
  14. Kroppenstedt R. M. 1985; Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics173–199 Goodfellow M., Minnikin D. E. London & New York: Academic Press;
    [Google Scholar]
  15. Kroppenstedt R. M., Korn-Wendisch F., Fowler V. J., Stackebrandt E. 1981; Biochemical and molecular genetic evidence for a transfer of of Actinoplanes armeniacus into the family Streptomycetaceae. Zentbl Bakteriol Hyg I Abt Orig C2:254–262
    [Google Scholar]
  16. Lechevalier M. P., Gerber N. N. 1970; The identity of 3-O-methyl-d-galactose with madurose. Carbohydr Res 13:451–154
    [Google Scholar]
  17. Lechevalier H. A., Lechevalier M. P. 1970; A critical evaluation of the genera of aerobic actinomycetes. In The Actinomycetales393–405 Prauser H. Jena: Gustav Fischer Verlag;
    [Google Scholar]
  18. Lechevalier M. P., de Biévre C., Lechevalier H. A. 1977; Chemotaxonomy of aerobic actinomycetes : Phospholipid composition. Biochem Ecol Syst 5:249–260
    [Google Scholar]
  19. Lechevalier M. P., Stern A. E., Lechevalier H. A. 1981; Phospholipids in the taxonomy of actinomycetes. Zentbl Bakteriol Parasitenkd Infektionskr Hyg I Abt Suppl 11:111–116
    [Google Scholar]
  20. Maidak B. L., Larsen N., McCaughey M. J., Overbeek R., Olsen G. J., Fogel K., Blandy J., Woese C. R. 1994; The Ribosomal Database Project. Nucleic Acids Res 22:3485–3487
    [Google Scholar]
  21. Maroni M., Seifert B., Lindvall T. 1995; In Air Quality Monographs. 3149–160 Amsterdam: Elsevier;
    [Google Scholar]
  22. Miller L., Berger T. 1984; Bacterial identification by gas chromatography and whole cell fatty acids. Gas Chromatography Application Note228–41 Hewlett Packard;
    [Google Scholar]
  23. Minnikin D. E., Alshamaony L., Goodfellow M. 1975; Differentiation of Mycobacterium, Nocardia and related taxa by thin-layer chromatographic analysis of whole-organism methanolysates. J Gen Microbiol 88:200–204
    [Google Scholar]
  24. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Parlett J. H. 1984; An integrated procedure for extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241
    [Google Scholar]
  25. Nohynek L. J., Suhonen E. L., Nurmiaho-Lassila E.-L., Hantula J., Salkinoja-Salonen M. 1995; Description of four pentachlorophenol-degrading bacterial strains as Sphingomonas chlorophenolica sp. nov. Syst Appl Microbiol 18:527–538
    [Google Scholar]
  26. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E. 1996; The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46:1088–1092
    [Google Scholar]
  27. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20:1–6
    [Google Scholar]
  28. Stackebrandt E., Rainey F. A., Ward-Rainey N. L. 1997; Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47:479–491
    [Google Scholar]
  29. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin layer chromatography. Appl Microbiol 28:226–231
    [Google Scholar]
  30. Uchida K., Aida K. 1977; Acyl type of bacterial cell wall: its simple identification by colorimetric method. J Gen Appl Microbiol 23:249–260
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-49-2-681
Loading
/content/journal/ijsem/10.1099/00207713-49-2-681
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error