RT Journal Article SR Electronic(1) A1 Rheims, Holger A1 Frühling, Anja A1 Schumann, Peter A1 Rohde, Manfred A1 Stackebrandt, ErkoYR 1999 T1 Bacillus silvestris sp. nov., a new member of the genus Bacillus that contains lysine in its cell wall JF International Journal of Systematic and Evolutionary Microbiology, VO 49 IS 2 SP 795 OP 802 DO https://doi.org/10.1099/00207713-49-2-795 PB Microbiology Society, SN 1466-5034, AB A Gram-positive, aerobic, rod-shaped, peritrichously flagellated, roundendospore-forming bacterium was isolated from a forest soil near Braunschweig, Lower Saxony, Germany, and designated strain HR3-23T(T = type strain). Morphologically, strain HR3-23T shows the characteristics of a member of the genus Bacillus. The spore position is terminal in a swollen sporangium. Comparative analysis of the 16S rDNA sequence shows strain HR3-23T to be most closely related to Caryophanon tenue (95·8% 16S rRNA similarity) and to Bacillus sphaericus (95·4% 16S rRNA similarity). Phylogenetically, the isolate clusters among species of Bacillus RNA group 2. The DNA G+C content of isolate HR3-23T is 39·3 mol%, the peptidoglycan type is A4α (l-Lys-d-Glu), the major respiratory lipoquinone is menaquinone MK-7 and the predominant fatty acid is of the iso-C15:0 type. Based on the morphological, chemotaxonomic, physiological and phylogenetic properties, a new species, Bacillus silvestris, is proposed; strain HR3-23Tis the type strain (= DSM 12223T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-49-2-795