1887

Abstract

The taxonomic position of a streptomycete strain isolated from Malaysian soil was established using a polyphasic approach. The organism, designated strain ATB-11, was found to have chemical and morphological properties consistent with its classification in the genus . An almost complete 16S rRNA gene (rDNA) sequence determined for the test strain was compared with those of previously studied streptomycetes by using two treeing algorithms. The 16S rDNA sequence data not only supported classification of the strain in the genus but also showed that it formed a distinct phyletic line At maturity, the aerial hyphae of strain ATB-11 differentiated into tight spira chains of rugose, cylindrical spores. The organism was readily distinguished from representatives of validly described species with rugose spores by using a combination of phenotypic features. It is proposed, therefore, that strain ATB-11 be classified in the genus as sp. nov.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-49-4-1395
1999-10-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/49/4/ijs-49-4-1395.html?itemId=/content/journal/ijsem/10.1099/00207713-49-4-1395&mimeType=html&fmt=ahah

References

  1. Al-Tai A. M., Vikineswary S., Saravana M. K., Wong W. H. 1995 Characterisation of actinomycetes from Tasek Bera- ‘Malaysia’s first ramsar site’. International Conference on Wetlands and Development; Selangor, Malaysia: Abstract no. 273 2.1
    [Google Scholar]
  2. Berdy J. 1995; Are actinomycetes exhausted as a source of secondary metabolites?. Biotechnologia7–813–34
    [Google Scholar]
  3. Chun J. 1995 Computer-assisted classification and identification of actinomycetes PhD thesis University of Newcastle upon Tyne;
    [Google Scholar]
  4. Chun J., Goodfellow M. 1995; A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45:240–245
    [Google Scholar]
  5. Chun J., Blackall L. L, Kang S.-O., Hah Y. C., Goodfellow M. 1997a; A proposal to reclassify Nocardiapinensis Blackall et al. as Skermania piniformis gen. nov., comb. nov. Int J Syst Bacteriol 47:127–131
    [Google Scholar]
  6. Chun J., Youn H.-D., Yim Y. I., Lee H., Kim M. Y., Hah Y. C., Kang S.-O. 1997b; Streptomyces seoulensis sp. nov. Int J Syst Bacteriol 47:492–498
    [Google Scholar]
  7. Cowan S. T., Steel K. L. 1974 Manual for the Identification of Medical Bacteria. , 2nd. Cambridge: Cambridge University Press;
    [Google Scholar]
  8. Embley M. T., Stackebrandt E. 1994; The molecular phylogeny and systematics of actinomycetes. Annu Rev Microbiol 48:257–289
    [Google Scholar]
  9. Felsenstein J. 1993 phylip (phylogenetic inference package), version 3.5c. Department of Genetics, University of Washington; Seattle, WA, USA:
    [Google Scholar]
  10. Fitch W. M., Margoliash E. 1967; Construction of phylogenetic trees: a method based on mutation distances as estimated from cytochrome c sequences is of general applicability. Science 155:279–284
    [Google Scholar]
  11. Goodfellow IVL, Manfio G. P., Chun J. 1997 Towards a practical species concept for cultivable bacteria. Species: the Units of Biodiversity25–69 Edited by Claridge M. F., Dawah H. A., Wilson M. R. London: Chapman & Hall;
    [Google Scholar]
  12. Gordon R. E., Barnett D. A. 1977; Resistance to rifampin and lysozyme of strains of some species of Mycobacterium and Nocardia as a taxonomic tool. Int J Syst Bacteriol 27:176–178
    [Google Scholar]
  13. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H. N. 1974; Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24:54–63
    [Google Scholar]
  14. Jones K. L. 1949; Fresh isolates of actinomycetes in which the presence of sporogenous aerial mycelia is a fluctuating characteristic. J Bacteriol 57:141–146
    [Google Scholar]
  15. Jukes T. H., Cantor C. R. 1969 Evolution of protein molecules. Mammalian Protein Metabolism 321–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  16. Kâmpfer P., Kroppenstedt R. M., Dot W. 1991; A numerical classification of the genera Streptomyces and Streptoverticillium using miniaturized physiological tests. J Gen Microbiol 137:1831–1891
    [Google Scholar]
  17. Kim B., Sahin N., Minnikin D. E., Zakrzewska-Czerwinska J., Mordarski M., Goodfellow M. 1999; Classification of thermophilic streptomycetes including the description of Streptomyces thermoalcalitolerans sp. nov. Int J Syst Bacteriol 49:7–17
    [Google Scholar]
  18. Kim D., Chun J., Sahin N., Hah Y. C., Goodfellow M. 1996; Analysis of thermophilic clades within the genus Streptomyces by 16S ribosomal DNA sequence comparisons. Int J Syst Bacteriol 46:581–587
    [Google Scholar]
  19. Kim S. B., Falconer C., Williams E., Goodfellow M. 1998; Streptomyces thermocarboxydovorans sp. nov. and Streptomyces thermocarboxydus sp. nov., two moderately thermophilic carboxydotrophic species from soil. Int J Syst Bacteriol 48:59–68
    [Google Scholar]
  20. Kroppenstedt R. M. 1985 Fatty acid and menaquinone analysis of actinomycetes and related organisms. Chemical Methods in Bacterial Systematics173–199 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  21. Labeda D. P., Lyons A. J. 1991; The Streptomyces violaceusniger cluster is heterogeneous in DNA relatedness among strains: emendation of the description of 5. violaceusniger and Streptomyces hygroscopicus. Int J Syst Bacteriol 41:398–401
    [Google Scholar]
  22. Labeda D. P., Lechevalier M. P., Testa R. T. 1997; Streptomyces stramineus sp. nov., a new species of the verticillate streptomycetes. Int J Syst Bacteriol 47:747–753
    [Google Scholar]
  23. Lechevalier M. P., Lechevalier H. A. 1970; Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20:435–443
    [Google Scholar]
  24. Lechevalier M. P., Stern A. E., Lechevalier H. A. 1977; Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5:249–260
    [Google Scholar]
  25. Maidak B. L, Olsen G. J., Larsen N., Overbeek R., McGaughey M. J., Woese G. R. 1997; The Ribosomal Database Project (RDP). Nucleic Acids Res 25:109–111
    [Google Scholar]
  26. Manfio G. P. 1995 Towards minimal standards for the description of Streptomyces species PhD thesis University of Newcastle upon Tyne;
    [Google Scholar]
  27. Manfio G. P., Zakrzewska-Czerwinska J., Atalan E., Goodfellow M. 1995; Towards minimal standards for the description of Streptomyces species. Biotechnologia 7-8:242–253
    [Google Scholar]
  28. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M. 1980; Thin-layer chromatography of methanolysates of mycolic acid containing bacteria. J Chromatogr 188:221–233
    [Google Scholar]
  29. Minnikin D. E., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241
    [Google Scholar]
  30. Ochi K. 1995; A taxonomic study of the genus Streptomyces by analysis of ribosomal protein AT-L30. Int J Syst Bacteriol 45:507–514
    [Google Scholar]
  31. O’Donnell A. G., Falconer C., Goodfellow M., Ward A. C., Williams E. 1993; Biosystematics and diversity amongst novel carboxydotrophic actinomycetes. Antonie Leeuwenhoek 64:325–340
    [Google Scholar]
  32. Pitcher D. G., Saunders N. A., Owen R. J. 1989; Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 8:151–156
    [Google Scholar]
  33. Saddler G. S., Tavecchia P., Locuiro S., Zanol M., Colombo L., Selva E. 1991; Analysis of madurose and other actinomycete whole cell sugars by gas chromatography. J Microbiol Methods 14:185–191
    [Google Scholar]
  34. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  35. Sneath P. H. A. 1980; basic program for the most diagnostic properties of groups from an identification matrix of percent positive characters. Comp Geosci 6:21–26
    [Google Scholar]
  36. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231
    [Google Scholar]
  37. Swofford D. L., Olsen G. J. 1990 Phylogenetic reconstruction. Molecular Systematics411–501 Edited by Hillis D., Moritz C. Sunderland, MA: Sinauer Associates;
    [Google Scholar]
  38. Tresner H. D., Hayes J. A., Backus E. J. 1968; Differential tolerance of streptomycetes to sodium chloride as a taxonomic aid. Appl Microbiol 16:1134–1136
    [Google Scholar]
  39. Waksman S.A., Henrici A. T. 1943; The nomenclature and classification of the actinomycetes. J Bacteriol 46:337–341
    [Google Scholar]
  40. Williams S. T., Goodfellow M., Alderson G., Wellington E. M. H., Sneath P. H. A., Sackin M. J. 1983; Numerical classification of Streptomyces and related genera. J Gen Microbiol 129:1743–1813
    [Google Scholar]
  41. Williams S. T., Goodfellow M., Alderson G. 1989 Genus Streptomyces Waksman and Henrici, 1943, 339AL. Bergey’s Manual of Systematic Bacteriology 42452–2492 Edited by Williams S. T., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-49-4-1395
Loading
/content/journal/ijsem/10.1099/00207713-49-4-1395
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error