%0 Journal Article %A Phongsopitanun, Wongsakorn %A Tanasupawat, Somboon %A Suwanborirux, Khanit %A Ohkuma, Moriya %A Kudo, Takuji %T Actinomadura rayongensis sp. nov., isolated from peat swamp forest soil %D 2015 %J International Journal of Systematic and Evolutionary Microbiology, %V 65 %N Pt_3 %P 890-895 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.000033 %I Microbiology Society, %X A novel actinomycete strain RY35-68T, isolated from a peat swamp forest soil sample in Rayong Province, Thailand, was characterized using a polyphasic approach. The strain belonged to the genus Actinomadura based on morphological and chemotaxonomic characteristics. Cell-wall analysis revealed the presence of meso-diaminopimelic acid and N-acetylmuramic acid in the peptidoglycan layer. The diagnostic sugar in whole-cell hydrolysates was identified as madurose. The predominant menaquinones were MK-9(H6), MK-9(H8) and MK-9(H4). The major cellular fatty acids were C16 : 0 and iso-C16 : 0. The major polar lipids were diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside. The genomic DNA G+C content was 73.7 mol%. On the basis of 16S rRNA gene sequence similarity analysis, strain RY35-68T was closely related to the species Actinomadura atramentaria JCM 6250T (97.5 %). The value of DNA–DNA relatedness between strain RY35-68T and A. atramentaria JCM 6250T was 37.6–42.6 %. On the basis of its phenotypic characteristics and these results mentioned, this strain could be distinguished from the closely related type strain and represents a novel species of the genus Actinomadura , for which the name Actinomadura rayongensis sp. nov. (type strain RY35-68T = JCM 19830T = TISTR 2211T = PCU 332T) is proposed. %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.000033