1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 65, Issue Pt_3

sp. nov., a member of the – complex isolated from human clinical specimens Free

Abstract

This study aimed to define the taxonomic status of a phenetically distinct group of 16 strains that corresponds to genomic species ‘close to 13TU’, a provisional genomic species of the – (ACB) complex recognized by Gerner-Smidt and Tjernberg in 1993. These strains have been isolated in different countries since the early 1990s and were mostly recovered from human clinical specimens. They were compared with 45 reference strains representing the known taxa of the ACB complex using taxonomic methods relevant to the genus . Based on sequence analysis of the concatenated partial sequences (2976 bp) of seven housekeeping genes, the 16 strains formed a tight and well-supported cluster (intracluster sequence identity of ≥98.4 %) that was clearly separated from the other members of the ACB complex (≤94.7 %). The species status of the group was supported by average nucleotide identity values of ≤91.7 % between the whole genome sequence of representative strain NIPH 973 (NCBI accession no. APOO00000000) and those of the other species. In addition, whole-cell matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) MS analyses indicated the distinctness of the group at the protein level. Metabolic and physiological tests revealed several typical features of the group, although they did not allow its reliable differentiation from the other members of the ACB complex. We conclude that the 16 strains represent a distinct novel species, for which we propose the name sp. nov. The type strain is NIPH 973 ( = CIP 110471 = CCUG 34785 = CCM 8535).

  • Published Online:
Funding
This study was supported by the:
  • Czech Science Foundation (Award 13-26693S)
© 2015 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000043
2015-03-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/3/934.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000043&mimeType=html&fmt=ahah

References

  1. Baumann P., Doudoroff M., Stanier R. Y. ( 1968 ). A study of the Moraxella group. II. Oxidative-negative species (genus Acinetobacter). . J Bacteriol 95, 1520–1541.[PubMed]
    [Google Scholar]
  2. Bouvet P. J. M., Grimont P. A. D. ( 1986 ). Taxonomy of the genus Acinetobacter with the recognition of Acinetobacter baumannii sp. nov., Acinetobacter haemolyticus sp. nov., Acinetobacter johnsonii sp. nov., and Acinetobacter junii sp. nov. and emended descriptions of Acinetobacter calcoaceticus and Acinetobacter lwoffii . . Int J Syst Bacteriol 36, 228–240. [View Article]
    [Google Scholar]
  3. Chan J. Z., Halachev M. R., Loman N. J., Constantinidou C., Pallen M. J. ( 2012 ). Defining bacterial species in the genomic era: insights from the genus Acinetobacter . . BMC Microbiol 12, 302. [View Article] [PubMed]
    [Google Scholar]
  4. Diancourt L., Passet V., Nemec A., Dijkshoorn L., Brisse S. ( 2010 ). The population structure of Acinetobacter baumannii: expanding multiresistant clones from an ancestral susceptible genetic pool. . PLoS ONE 5, e10034. [View Article] [PubMed]
    [Google Scholar]
  5. Gerner-Smidt P., Tjernberg I. ( 1993 ). Acinetobacter in Denmark: II. Molecular studies of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex. . APMIS 101, 826–832. [View Article] [PubMed]
    [Google Scholar]
  6. Gerner-Smidt P., Tjernberg I., Ursing J. ( 1991 ). Reliability of phenotypic tests for identification of Acinetobacter species. . J Clin Microbiol 29, 277–282.[PubMed]
    [Google Scholar]
  7. Houang E. T., Chu Y. W., Leung C. M., Chu K. Y., Berlau J., Ng K. C., Cheng A. F. ( 2001 ). Epidemiology and infection control implications of Acinetobacter spp. in Hong Kong. . J Clin Microbiol 39, 228–234. [View Article] [PubMed]
    [Google Scholar]
  8. Juni E. ( 1972 ). Interspecies transformation of Acinetobacter: genetic evidence for a ubiquitous genus. . J Bacteriol 112, 917–931.[PubMed]
    [Google Scholar]
  9. Karah N., Haldorsen B., Hegstad K., Simonsen G. S., Sundsfjord A., Samuelsen Ø. Norwegian Study Group of Acinetobacter ( 2011 ). Species identification and molecular characterization of Acinetobacter spp. blood culture isolates from Norway. . J Antimicrob Chemother 66, 738–744. [View Article] [PubMed]
    [Google Scholar]
  10. Kim D. H., Park Y. K., Choi J. Y., Ko K. S. ( 2012 ). Identification of genetic recombination between Acinetobacter species based on multilocus sequence analysis. . Diagn Microbiol Infect Dis 73, 284–286. [View Article] [PubMed]
    [Google Scholar]
  11. Krizova L., Maixnerova M., Sedo O., Nemec A. ( 2014 ). Acinetobacter bohemicus sp. nov. widespread in natural soil and water ecosystems in the Czech Republic. . Syst Appl Microbiol 37, 467–473. [View Article] [PubMed]
    [Google Scholar]
  12. Krizova L., McGinnis J., Maixnerova M., Nemec M., Poirel L., Mingle L., Sedo O., Wolfgang W., Nemec A. ( 2015 ). Acinetobacter variabilis sp. nov. (formerly DNA group 15 sensu Tjernberg and Ursing), isolated from humans and animals. . Int J Syst Evol Microbiol (in press). [View Article] [PubMed]
    [Google Scholar]
  13. Nemec A., Janda L., Melter O., Dijkshoorn L. ( 1999 ). Genotypic and phenotypic similarity of multiresistant Acinetobacter baumannii isolates in the Czech Republic. . J Med Microbiol 48, 287–296. [View Article] [PubMed]
    [Google Scholar]
  14. Nemec A., Musílek M., Maixnerová M., De Baere T., van der Reijden T. J. K., Vaneechoutte M., Dijkshoorn L. ( 2009 ). Acinetobacter beijerinckii sp. nov. and Acinetobacter gyllenbergii sp. nov., haemolytic organisms isolated from humans. . Int J Syst Evol Microbiol 59, 118–124. [View Article] [PubMed]
    [Google Scholar]
  15. Nemec A., Musílek M., Sedo O., De Baere T., Maixnerová M., van der Reijden T. J., Zdráhal Z., Vaneechoutte M., Dijkshoorn L. ( 2010 ). Acinetobacter bereziniae sp. nov. and Acinetobacter guillouiae sp. nov., to accommodate Acinetobacter genomic species 10 and 11, respectively. . Int J Syst Evol Microbiol 60, 896–903. [View Article] [PubMed]
    [Google Scholar]
  16. Nemec A., Krizova L., Maixnerova M., van der Reijden T. J. K., Deschaght P., Passet V., Vaneechoutte M., Brisse S., Dijkshoorn L. ( 2011 ). Genotypic and phenotypic characterization of the Acinetobacter calcoaceticus–Acinetobacter baumannii complex with the proposal of Acinetobacter pittii sp. nov. (formerly Acinetobacter genomic species 3) and Acinetobacter nosocomialis sp. nov. (formerly Acinetobacter genomic species 13TU). . Res Microbiol 162, 393–404. [View Article] [PubMed]
    [Google Scholar]
  17. Peleg A. Y., de Breij A., Adams M. D., Cerqueira G. M., Mocali S., Galardini M., Nibbering P. H., Earl A. M., Ward D. V. & other authors ( 2012 ). The success of Acinetobacter species; genetic, metabolic and virulence attributes. . PLoS ONE 7, e46984. [View Article] [PubMed]
    [Google Scholar]
  18. Périchon B., Goussard S., Walewski V., Krizova L., Cerqueira G., Murphy C., Feldgarden M., Wortman J., Clermont D. & other authors ( 2014 ). Identification of 50 class D β-lactamases and 65 Acinetobacter-derived cephalosporinases in Acinetobacter spp.. Antimicrob Agents Chemother 58, 936–949. [View Article] [PubMed]
    [Google Scholar]
  19. Richter M., Rosselló-Móra R. ( 2009 ). Shifting the genomic gold standard for the prokaryotic species definition. . Proc Natl Acad Sci U S A 106, 19126–19131. [View Article] [PubMed]
    [Google Scholar]
  20. Šedo O., Nemec A., Křížová L., Kačalová M., Zdráhal Z. ( 2013 ). Improvement of MALDI-TOF MS profiling for the differentiation of species within the Acinetobacter calcoaceticus–Acinetobacter baumannii complex. . Syst Appl Microbiol 36, 572–578. [View Article] [PubMed]
    [Google Scholar]
  21. Tjernberg I., Ursing J. ( 1989 ). Clinical strains of Acinetobacter classified by DNA-DNA hybridization. . APMIS 97, 595–605. [View Article] [PubMed]
    [Google Scholar]
  22. Touchon M., Cury J., Yoon E. J., Krizova L., Cerqueira G. C., Murphy C., Feldgarden M., Wortman J., Clermont D. & other authors ( 2014 ). The genomic diversification of the whole Acinetobacter genus: origins, mechanisms, and consequences. . Genome Biol Evol 6, 2866–2882. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000043
Loading
Acinetobacter seifertii sp. nov., a member of the Acinetobacter calcoaceticus–Acinetobacter baumannii complex isolated from human clinical specimens
Int J Syst Evol Microbiol 65, 934 (2015); https://doi.org/10.1099/ijs.0.000043
/content/journal/ijsem/10.1099/ijs.0.000043
/content/journal/ijsem/10.1099/ijs.0.000043
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error