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Volume 65, Issue Pt_4

gen. nov., sp. nov., a chemolithoautotrophic, thermophilic, nitrate-ammonifying bacterium from a deep-sea hydrothermal vent Free

Abstract

A thermophilic, anaerobic, chemolithoautotrophic bacterium, strain TB-6, was isolated from a deep-sea hydrothermal vent located on the East Pacific Rise at 9° N. The cells were Gram-staining-negative and rod-shaped with one or more polar flagella. Cell size was approximately 1–1.5 µm in length and 0.5 µm in width. Strain TB-6 grew between 45 and 70 °C (optimum 55–60 °C), 0 and 35 g NaCl l (optimum 20–30 g l) and pH 4.5 and 7.5 (optimum pH 5.5–6.0). Generation time under optimal conditions was 2 h. Growth of strain TB-6 occurred with H as the energy source, CO as the carbon source and nitrate or sulfur as electron acceptors, with formation of ammonium or hydrogen sulfide, respectively. Acetate, (+)--glucose, Casamino acids, sucrose and yeast extract were not used as carbon and energy sources. Inhibition of growth occurred in the presence of lactate, peptone and tryptone under a H/CO (80 : 20; 200 kPa) gas phase. Thiosulfate, sulfite, arsenate, selenate and oxygen were not used as electron acceptors. The G+C content of the genomic DNA was 36.8 mol%. Phylogenetic analysis of the 16S rRNA gene of strain TB-6 showed that this organism branched separately from the three most closely related genera, , and , within the family . Strain TB-6 contained several unique fatty acids in comparison with other members of the family . Based on experimental evidence, it is proposed that the organism represents a novel species and genus within the family gen. nov., sp. nov. The type strain is TB-6 ( = DSM 27783 = JCM 19563).

  • Published Online:
Funding
This study was supported by the:
  • National Science Foundation (Award OCE 11-24141, MCB 08-43678 and OCE 11-36451)
© 2015 IUMS
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2015-04-01
2024-04-25
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Cetia pacifica gen. nov., sp. nov., a chemolithoautotrophic, thermophilic, nitrate-ammonifying bacterium from a deep-sea hydrothermal vent
Int J Syst Evol Microbiol 65, 1144 (2015); https://doi.org/10.1099/ijs.0.000070
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