1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 65, Issue Pt_4

sp. nov. and sp. nov., two spore-forming lactic acid bacteria isolated from tree barks in Thailand Free

Abstract

Two Gram-stain-positive, endospore-forming lactic acid bacteria, designated BK92 and BK117-1, were isolated from tree barks in Thailand. Cells were catalase-negative and facultatively anaerobic rods. 16S rRNA gene sequence analysis indicated that these strains belonged to the genus . Strains BK92 and BK117-1 showed the highest 16S rRNA gene sequence similarity to QC81-06 with 97.7 % and 97.1 % similarity, respectively. Analysis of phylogenetic relationships based on 16S rRNA and gene sequencing revealed that the positions of strains BK92 and BK117-1 were clearly separated from all related species of the genus . Strains BK92 and BK117-1 had low DNA–DNA relatedness between each other and also with QC81-06 and SL153. The DNA G+C content of strains BK92 and BK117-1 was 46.6 mol% and 47.4 mol%, respectively. The major fatty acids of strains BK92 and BK117-1 were anteiso-C and anteiso-C. They contained -diaminopimelic acid in cell-wall peptidoglycan and had menaquinone with seven isoprene units (MK-7) as the predominant menaquinone. Based on evidence including phenotypic, genotypic and chemotaxonomic studies, strains BK92 and BK117-1 should be classified as representatives of novel species of the genus , for which the names sp. nov. and sp. nov. are proposed, respectively. The type strains are BK92 ( = JCM 19541 = LMG 28365 = PCU 336 = TISTR 2234) and BK117-1 ( = JCM 19542 = LMG 28366 = PCU 337 = TISTR 2235).

  • Published Online:
Funding
This study was supported by the:
  • Thailand Research Fund
  • Chulalongkorn University
© 2015 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000084
2015-04-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/4/1220.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000084&mimeType=html&fmt=ahah

References

  1. Andersch I., Pianka S., Fritze D., Claus D. ( 1994 ). Description of Bacillus laevolacticus (ex Nakayama and Yanoshi 1967) sp. nov., nom. rev.. Int J Syst Bacteriol 44, 659–664. [View Article]
    [Google Scholar]
  2. Chang Y. H., Jung M. Y., Park I. S., Oh H. M. ( 2008 ). Sporolactobacillus vineae sp. nov., a spore-forming lactic acid bacterium isolated from vineyard soil. . Int J Syst Evol Microbiol 58, 2316–2320. [View Article] [PubMed]
    [Google Scholar]
  3. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. ( 1977 ). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100, 221–230. [View Article] [PubMed]
    [Google Scholar]
  4. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39, 224–229. [View Article]
    [Google Scholar]
  5. Fujita R., Mochida K., Kato Y., Goto K. ( 2010 ). Sporolactobacillus putidus sp. nov., an endospore-forming lactic acid bacterium isolated from spoiled orange juice. . Int J Syst Evol Microbiol 60, 1499–1503. [View Article] [PubMed]
    [Google Scholar]
  6. Hatayama K., Shoun H., Ueda Y., Nakamura A. ( 2006 ). Tuberibacillus calidus gen. nov., sp. nov., isolated from a compost pile and reclassification of Bacillus naganoensis Tomimura et al. 1990 as Pullulanibacillus naganoensis gen. nov., comb. nov. and Bacillus laevolacticus Andersch et al. 1994 as Sporolactobacillus laevolacticus comb. nov.. Int J Syst Evol Microbiol 56, 2545–2551. [View Article] [PubMed]
    [Google Scholar]
  7. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716–721.[PubMed] [CrossRef]
    [Google Scholar]
  8. Kitahara K., Suzuki J. ( 1963 ). Sporolactobacillus nov. subgen.. J Gen Appl Microbiol 9, 59–71. [View Article]
    [Google Scholar]
  9. Kitahara M., Takamine F., Imamura T., Benno Y. ( 2001 ). Clostridium hiranonis sp. nov., a human intestinal bacterium with bile acid 7α-dehydroxylating activity. . Int J Syst Evol Microbiol 51, 39–44.[PubMed] [CrossRef]
    [Google Scholar]
  10. Kitahara M., Sakamoto M., Benno Y. ( 2010 ). Lactobacillus similis sp. nov., isolated from fermented cane molasses. . Int J Syst Evol Microbiol 60, 187–190. [View Article] [PubMed]
    [Google Scholar]
  11. Ludwig W., Schleifer K. H., Whitman W. B. ( 2009 ). Genus Sporolactobacillus . . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 3, pp. 386–391. Edited by De Vos P., Garrity G. M., Jones D., Krieg N. R., Ludwig W., Rainey F. A., Schleifer K.-H., Whitman W. B. . New York:: Springer;.
     [Google Scholar]
  12. Matsuo Y., Katsuta A., Matsuda S., Shizuri Y., Yokota A., Kasai H. ( 2006 ). Mechercharimyces mesophilus gen. nov., sp. nov. and Mechercharimyces asporophorigenens sp. nov., antitumour substance-producing marine bacteria, and description of Thermoactinomycetaceae fam. nov.. Int J Syst Evol Microbiol 56, 2837–2842. [View Article] [PubMed]
    [Google Scholar]
  13. Nicholson W., Setlow P. ( 1990 ). Sporulation, germination and outgrowth. . In Molecular Biological Methods for Bacillus, pp. 391–450. Edited by Harwood C., Cutting S. . New York:: Wiley;.
     [Google Scholar]
  14. Oki K., Kudo Y., Watanabe K. ( 2012 ). Lactobacillus saniviri sp. nov. and Lactobacillus senioris sp. nov., isolated from human faeces. . Int J Syst Evol Microbiol 62, 601–607. [View Article] [PubMed]
    [Google Scholar]
  15. Parte A. C. . ( 2014 ). List of prokaryotic names with standing in nomenclature: a folder available on the Internet. [Last full update 17 June 2014]. http://www.bacterio.net
  16. Prasirtsak B., Tanasupawat S., Boonsombat R., Kodama K., Thongchul N. ( 2013 ). Characterization of lactic acid producing bacteria from Thai sources. . J Appl Pharm Sci 3, 33–38.
     [Google Scholar]
  17. Saito H., Miura K. I. ( 1963 ). Preparation of transforming deoxyribonucleic acid by phenol treatment. . Biochim Biophys Acta 72, 619–629. [View Article] [PubMed]
    [Google Scholar]
  18. Sakamoto M., Suzuki M., Umeda M., Ishikawa I., Benno Y. ( 2002 ). Reclassification of Bacteroides forsythus (Tanner et al. 1986) as Tannerella forsythensis corrig., gen. nov., comb. nov.. Int J Syst Evol Microbiol 52, 841–849. [View Article] [PubMed]
    [Google Scholar]
  19. Slepecky R., Foster J. W. ( 1959 ). Alterations in metal content of spores of Bacillus megaterium and the effect on some spore properties. . J Bacteriol 78, 117–123.[PubMed]
    [Google Scholar]
  20. Staneck J. L., Roberts G. D. ( 1974 ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol 28, 226–231.[PubMed]
    [Google Scholar]
  21. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 2731–2739. [View Article] [PubMed]
    [Google Scholar]
  22. Tanasupawat S., Thongsanit J., Okada S., Komagata K. ( 2002 ). Lactic acid bacteria isolated from soy sauce mash in Thailand. . J Gen Appl Microbiol 48, 201–209. [View Article] [PubMed]
    [Google Scholar]
  23. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. . ( 1997 ). The CLUSTAL_X windows interface: Flexible strategies for multiple sequence alignmentaided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.
  24. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. ( 1987 ). International committee on Systematic Bacteriology. Report of the ad hoc committee on the reconciliation of approaches to bacterial systematic. . Int J Syst Bacteriol 37, 463–464. [View Article]
    [Google Scholar]
  25. Yanagida F., Suzuki K., Kaneko T., Kozaki M., Komagata K. ( 1987 ). Morphological, biochemical, and physiological characteristics of spore-forming lactic acid bacteria. . J Gen Appl Microbiol 33, 33–45. [View Article]
    [Google Scholar]
  26. Yanagida F., Suzuki K., Kozaki M., Komagata K. ( 1997 ). Proposal of Sporolactobacillus nakayamae subsp. nakayamae sp. nov., subsp. nov., Sporolactobacillus nakayamae subsp. racemicus subsp. nov., Sporolactobacillus terrae sp. nov., Sporolactobacillus kofuensis sp. nov., and Sporolactobacillus lactosus sp. nov.. Int J Syst Bacteriol 47, 499–504. [View Article]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000084
Loading
Sporolactobacillus shoreae sp. nov. and Sporolactobacillus spathodeae sp. nov., two spore-forming lactic acid bacteria isolated from tree barks in Thailand
Int J Syst Evol Microbiol 65, 1220 (2015); https://doi.org/10.1099/ijs.0.000084
/content/journal/ijsem/10.1099/ijs.0.000084
/content/journal/ijsem/10.1099/ijs.0.000084
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error