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Volume 65, Issue Pt_5

gen. nov., sp. nov., isolated from coastal sediment, and description of fam. nov. and ord. nov. Free

Abstract

A novel Gram-stain-negative, rod-shaped, gliding, facultatively anaerobic, oxidase-negative and catalase-positive bacterium, designated FA350, was isolated from coastal sediment from Xiaoshi Island, Weihai, China. Strain FA350 showed growth on modified nutrient agar supplemented with 0.1 % -(+)-trehalose and with distilled water replaced by seawater. Optimal growth occurred at 33 °C and pH 8.5 with 4 % NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain FA350 belongs to a novel bacterial order in the class , and the most closely related type strains belong to the order , with 85.1–85.6 % 16S rRNA gene sequence similarity. The polar lipid profile of the novel strain consisted of phosphatidylethanolamine, phosphatidylglycerol and two unknown phospholipids. Major cellular fatty acids were iso-C, iso-C and iso-Cω10 and menaquinone MK-7 was the sole respiratory quinone. The DNA G+C content of strain FA350 was 60.3 mol%. The isolate and closely related environmental clones formed a novel order-level clade in the class . Comparative analysis of 16S rRNA gene sequences and characterization indicated that strain FA350 may represent a novel order of the . Here, we propose the name gen. nov., sp. nov. to accommodate strain FA350. The type strain of is FA350 ( = DSM 28820 = CICC 10904); ord. nov. and fam. nov. are also proposed to accommodate the novel taxon.

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Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31370057 and 31290231)
  • National Science and Technology Major Project of China (Award 2013ZX10004217)
  • China Ocean Mineral Resources R & D Association (COMRA) Special Foundation (Award DY125-15-T-05)
© 2015 IUMS
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2015-05-01
2024-04-16
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Bradymonas sediminis gen. nov., sp. nov., isolated from coastal sediment, and description of Bradymonadaceae fam. nov. and Bradymonadales ord. nov.
Int J Syst Evol Microbiol 65, 1542 (2015); https://doi.org/10.1099/ijs.0.000135
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