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f gen. nov., sp. nov., a marine bacterium that reduces nitrate to nitrous oxide under strictly microaerobic conditions

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  • Authors: Mio Takeuchi1 ,  Takao Yamagishi1 ,  Yoichi Kamagata2 ,  Kenshiro Oshima3 ,  Masahira Hattori3 ,  Taiki Katayama1 ,  Satoshi Hanada2 ,  Hideyuki Tamaki2 ,  Katsumi Marumo4,† ,  Hiroto Maeda5 ,  Munetomo Nedachi6 ,  Wataru Iwasaki3,7 ,  Yuichi Suwa8 , Susumu Sakata1
    • VIEW AFFILIATIONS
    • 1 1Institute for Geo-resources and Environments, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8567, Japan 2 2Bioproduction Research Institute, AIST, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan 3 3The Department of Computational Biology, Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwa-no-ha, Kashiwa, Chiba 277-8561, Japan 4 4Institute of Geology and Geoinformation, AIST, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8567, Japan 5 5Department of Physics and Astronomy Graduate School of Science and Engineering/Faculty of Science, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-8580, Japan 6 6The United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-8580, Japan 7 7Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, Japan 8 8Department of Biological Science, Faculty of Science and Engineering, Chuo University, 1-13-27 Kasuga, Bunkyo-ku, Tokyo 112-8551, Japan
  • Mio Takeuchi [email protected]
  • First Published Online: 01 June 2015, International Journal of Systematic and Evolutionary Microbiology 65: 1749-1754, doi: 10.1099/ijs.0.000167
  • Subject: NEW TAXA - Proteobacteria
  • Cover date:
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  • A moderately thermophilic, aerobic, stalked bacterium (strain MA2) was isolated from marine sediments in Kagoshima Bay, Japan. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain MA2 was most closely related to the genera , and (92–93 % similarity) within the class . Strain MA2 was a Gram-stain-negative and stalked dimorphic bacteria. The temperature range for growth was 16–48 °C (optimum growth at 42 °C). This strain required yeast extract and NaCl (>1 %, w/v) for growth, tolerated up to 11 % (w/v) NaCl, and was capable of utilizing various carbon sources. The major cellular fatty acid and major respiratory quinone were Cω7 and ubiquinone-10, respectively. The DNA G+C content was 60.7 mol%. Strain MA2 performed denitrification and produced NO from nitrate under strictly microaerobic conditions. Strain MA2 possessed periplasmic nitrate reductase (Nap) genes but not membrane-bound nitrate reductase (Nar) genes. On the basis of this morphological, physiological, biochemical and genetic information a novel genus and species, gen. nov., sp. nov., are proposed, with MA2 ( = NBRC 109643 = DSM 27167) as the type strain of the species.

  • †

    Faculty of Science, Toyama University, 3190 Gofuku, Toyama-shi, Toyama 930-8555, Japan.

  • The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene of strain MA2 is AB821371. The draft genome sequence has been deposited in DDBJ/EMBL/GenBank under the accession numbers BBIO01000001–BBIO01000046.

  • Three supplementary figures are available with the online Supplementary Material.

  • Abbreviations:
    OMZ oxygen minimum zone;
    PE paired-end;
    SE single-end.

© 2015 IUMS | Published by the Microbiology Society

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