1887
  1. Home
  2. Publications
  3. International Journal of Systematic and Evolutionary Microbiology
  4. Volume 65, Issue 6
  5. Article

f sp. nov., a cellobiose-fermenting yeast species isolated from rotten wood

PDF
288.17 Kb
HTML
47.42 Kb
  • Cover date:
Preview this:
Free Preview thumbnail Magnify
Zoom in
Zoomout

sp. nov., a cellobiose-fermenting yeast species isolated from rotten wood, Page 1 of 1

| /docserver/preview/fulltext/ijsem/65/6/1755_ijs000169-1.gif

  • Two strains of an asexual cellobiose-fermenting yeast species were isolated from rotten wood samples collected in Funiu Mountain Nature Reserve in Henan Province, central China. Molecular phylogenetic analysis that included the nearly complete small subunit (SSU), the internal transcribed spacer (ITS) region and the D1/D2 domains of the large subunit (LSU) rDNA showed that these strains belonged to the clade, with and as their closest phylogenetic neighbours. The nucleotide differences between the novel strains and the type strains of . and . were 30 and 36 substitutions, respectively, in the D1/D2 LSU rDNA, 40 and 44 substitutions, respectively, in the ITS region and 19 and 23 substitutions, respectively, in the SSU rDNA. The novel strains can also be distinguished from their closest described species, . and . , by a number of physiological characteristics, and represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NYNU 14625 ( = CICC 33050 = CBS 13911). The Mycobank number is MB 811503.

  • †

    These authors contributed equally to this work.

  • The GenBank/EMBL/DDBJ accession number for the SSU rDNA, ITS region and D1/D2 LSU rDNA sequence of strain NYNU14625 are KM201407, KM201406 and KM201405, respectively.

  • Abbreviations:
    ITS internal transcribed spacer;
    LSU large subunit;
    SSU small subunit.

© 2015 IUMS | Published by the Microbiology Society

  • Altschul S. F. , Madden T. L. , Schäffer A. A. , Zhang J. , Zhang Z. , Miller W. , Lipman D. J. . ( 1997; ). Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. . Nucleic Acids Res 25:, 3389––3402. [CrossRef]
    [Google Scholar]
  • Barnett J. A. , Payne R. W. , Yarrow D. . ( 2000; ). Yeasts: Characteristics and Identification, , 3rd edn.. Cambridge:: Cambridge University Press;.
     [Google Scholar]
  • Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783––791. [CrossRef]
    [Google Scholar]
  • Galazka J. M. , Tian C. , Beeson W. T. , Martinez B. , Glass N. L. , Cate J. H. D. . ( 2010; ). Cellodextrin transport in yeast for improved biofuel production. . Science 330:, 84––86. [CrossRef] [PubMed]
    [Google Scholar]
  • Kimura M. . ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111––120. [CrossRef] [PubMed]
    [Google Scholar]
  • Kocková-Kratochvílová A. , Ondrušová D. . ( 1971; ). Torulopsis-arten aus den Oberflächen höherer Pilze. Torulopsis kruisii n. sp. und Torulopsis schatavii n. sp.. Biologia (Bratisl) 26:, 477––485.
     [Google Scholar]
  • Kurtzman C. P. , Robnett C. J. . ( 1998; ). Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. . Antonie van Leeuwenhoek 73:, 331––371. [CrossRef] [PubMed]
    [Google Scholar]
  • Kurtzman C. P. , Robnett C. J. . ( 2003; ). Phylogenetic relationships among yeasts of the ‘Saccharomyces complex’ determined from multigene sequence analyses. . FEMS Yeast Res 3:, 417––432. [CrossRef] [PubMed]
    [Google Scholar]
  • Kurtzman C. P. , Fell J. W. , Boekhout T. , Robert V. . ( 2011; ). Methods for isolation, phenotypic characterization and maintenance of yeasts. . In The Yeasts, a Taxonomic Study, , 5th edn., vol. 1, pp. 87––110. Edited by Kurtzman C. P. , Fell J. W. , Bekhout T. . . San Diego:: Elsevier;. [CrossRef]
    [Google Scholar]
  • Li S. , Du J. , Sun J. , Galazka J. M. , Glass N. L. , Cate J. H. D. , Yang X. , Zhao H. . ( 2010; ). Overcoming glucose repression in mixed sugar fermentation by co-expressing a cellobiose transporter and a β-glucosidase in Saccharomyces cerevisiae . . Mol Biosyst 6:, 2129––2132. [CrossRef] [PubMed]
    [Google Scholar]
  • Middelhoven W. J. , Kurtzman C. P. . ( 2007; ). Four novel yeasts from decaying organic matter: Blastobotrys robertii sp. nov., Candida cretensis sp. nov., Candida scorzettiae sp. nov. and Candida vadensis sp. nov.. Antonie van Leeuwenhoek 92:, 233––244. [CrossRef] [PubMed]
    [Google Scholar]
  • Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406––425.[PubMed]
    [Google Scholar]
  • Santos R. O. , Cadete R. M. , Badotti F. , Mouro A. , Wallheim D. O. , Gomes F. C. , Stambuk B. U. , Lachance M. A. , Rosa C. A. . ( 2011; ). Candida queiroziae sp. nov., a cellobiose-fermenting yeast species isolated from rotting wood in Atlantic Rain Forest. . Antonie van Leeuwenhoek 99:, 635––642. [CrossRef] [PubMed]
    [Google Scholar]
  • Sugita T. , Nakase T. . ( 1999; ). Non-universal usage of the leucine CUG codon and the molecular phylogeny of the genus Candida . . Syst Appl Microbiol 22:, 79––86. [CrossRef] [PubMed]
    [Google Scholar]
  • Suh S.-O. , Nguyen N. H. , Blackwell M. . ( 2006; ). A yeast clade near Candida kruisii uncovered: nine novel Candida species associated with basidioma-feeding beetles. . Mycol Res 110:, 1379––1394. [CrossRef] [PubMed]
    [Google Scholar]
  • Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011; ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731––2739. [CrossRef] [PubMed]
    [Google Scholar]
  • Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876––4882. [CrossRef] [PubMed]
    [Google Scholar]
  • White T. J. , Bruns T. , Lee S. , Taylor J. W. . ( 1990; ). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. . In PCR Protocols: a Guide to Methods and Applications, pp. 315––322. Edited by Innis M. A. , Gelfan D. H. , Sninsky J. J. , White T. J. . . New York:: Academic Press;. [CrossRef]
    [Google Scholar]
  • Yarrow D. , Meyer S. A. . ( 1978; ). Proposal for amendment of the diagnosis of the genus Candida Berkhout nom. cons. . Int J Syst Bacteriol 28:, 611––615. [CrossRef]
    [Google Scholar]
http://sgm.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000169
Loading

Supplementary Data

 Data loading....

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000169
2015-06-01
2019-02-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/6/1755.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000169&mimeType=html&fmt=ahah

Author and Article Information

 Figure data loading....

Footnotes:

Validated antibodies in this article

This is a required field
Please enter a valid email address
Please check the format of the address you have entered.
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error