@article{mbs:/content/journal/ijsem/10.1099/ijs.0.000172, author = "Suriyachadkun, Chanwit and Ngaemthao, Wipaporn and Chunhametha, Suwanee and Thawai, Chitti and Sanglier, Jean-Jacques", title = "Sinosporangium fuscum sp. nov., isolated from soil ", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2015", volume = "65", number = "Pt_6", pages = "1764-1769", doi = "https://doi.org/10.1099/ijs.0.000172", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.000172", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A novel actinomycete, A-T 8343T was isolated from a moist evergreen forest soil sample collected in the Trat Province, Thailand. Based on 16S rRNA gene sequence analysis, strain A-T 8343T belonged to the genus Sinosporangium and was closely related to Sinosporangium siamense A-T 1946T (98.81 %) and Sinosporangium album 6014T (98.54 %). The DNA–DNA relatedness values were 21.8–27 % with S. siamense A-T 1946T and 31.1–31.9 % with S. album 6014T, which were significantly below 70 %. The result differentiated A-T 8343T from the closest species. The organism developed spherical sporangia containing non-motile spores on aerial mycelia. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars contained rhamnose, ribose, madurose and glucose. The predominant menaquinones were MK-9(H2) and MK-9(H4). The diagnostic phospholipids were phosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxyl-phosphatidylethanolamine, diphosphatidylglycerol, lyso-phosphatidylethanolamine, N-acetylglucosamine-containing phospholipids, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol-mannosides, aminophosphoglycolipid and one unknown phospholipid. The major cellular fatty acids were saturated C16 : 0, iso C16 : 0, unsaturated C16 : 1 and C18 : 1. Following an evaluation of phenotypic, chemotaxonomic and genotypic characteristics, the new isolate is proposed as a representative novel species of the genus Sinosporangium to be named Sinosporangium fuscum sp. nov. The type strain is A-T 8343T ( = BCC 52770T = NBRC 109516T).", }