1887

Abstract

Two Gram-stain-negative, rod-shaped bacterial strains, cai42 and b45, were isolated from oil-production water taken from Xinjiang Oilfield, China. Optimum growth was observed at 30 °C, at pH 8 and with 1–3 % (w/v) NaCl. According to phylogenetic analyses, the two strains were members of the genus , with 16S rRNA gene sequence similarities of 95.5−96.3 % with the type strains of species of the genus. The major cellular fatty acids of strains cai42 and b45 were C 3-OH, C and summed feature 8 (Cω7/Cω6), and the predominant ubiquinone was Q-10, all of these data being typical for the genus . The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, glycolipid, phosphatidylcholine, two unidentified aminolipids, an unidentified phospholipid and two unidentified lipids. The mean genomic DNA G+C contents of strains cai42 and b45 were 60.8±1.1 and 60.4±1.0 mol%, respectively. On the basis of phylogenetic, physiological and chemotaxonomic analyses, strains cai42 and b45 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is cai42 ( = CGMCC 1.12518 = LMG 27406).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31225001)
  • National High Technology Research and Development Program (Award 2012AA02A703)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000181
2015-06-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/6/1805.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000181&mimeType=html&fmt=ahah

References

  1. Achenbach L. A., Carey J., Madigan M. T. ( 2001 ). Photosynthetic and phylogenetic primers for detection of anoxygenic phototrophs in natural environments. . Appl Environ Microbiol 67, 29222926. [View Article] [PubMed]
    [Google Scholar]
  2. Andrews J. M. BSAC Working Party on Susceptibility Testing ( 2008 ). BSAC standardized disc susceptibility testing method (version 7). . J Antimicrob Chemother 62, 256278. [View Article] [PubMed]
    [Google Scholar]
  3. Biebl H., Pukall R., Lünsdorf H., Schulz S., Allgaier M., Tindall B. J., Wagner-Döbler I. ( 2007 ). Description of Labrenzia alexandrii gen. nov., sp. nov., a novel alphaproteobacterium containing bacteriochlorophyll a, and a proposal for reclassification of Stappia aggregata as Labrenzia aggregata comb. nov., of Stappia marina as Labrenzia marina comb. nov. and of Stappia alba as Labrenzia alba comb. nov., and emended descriptions of the genera Pannonibacter, Stappia and Roseibium, and of the species Roseibium denhamense and Roseibium hamelinense . . Int J Syst Evol Microbiol 57, 10951107. [View Article] [PubMed]
    [Google Scholar]
  4. Cai M., Wang L., Cai H., Li Y., Wang Y. N., Tang Y. Q., Wu X. L. ( 2011 ). Salinarimonas ramus sp. nov. and Tessaracoccus oleiagri sp. nov., isolated from a crude oil-contaminated saline soil. . Int J Syst Evol Microbiol 61, 17671775. [View Article] [PubMed]
    [Google Scholar]
  5. De Ley J., Cattoir H., Reynaerts A. ( 1970 ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12, 133142. [View Article] [PubMed]
    [Google Scholar]
  6. Dong X. Z., Cai M. Y. ( 2001 ). Determinative Manual for Routine Bacteriology. Beijing:: Science Press;.
    [Google Scholar]
  7. Embley T. M. ( 1991 ). The linear PCR reaction: a simple and robust method for sequencing amplified rRNA genes. . Lett Appl Microbiol 13, 171174. [View Article] [PubMed]
    [Google Scholar]
  8. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [View Article] [PubMed]
    [Google Scholar]
  9. Foesel B. U., Drake H. L., Schramm A. ( 2011 ). Defluviimonas denitrificans gen. nov., sp. nov., and Pararhodobacter aggregans gen. nov., sp. nov., non-phototrophic Rhodobacteraceae from the biofilter of a marine aquaculture. . Syst Appl Microbiol 34, 498502. [View Article] [PubMed]
    [Google Scholar]
  10. Fraser S. L., Jorgensen J. H. ( 1997 ). Reappraisal of the antimicrobial susceptibilities of Chryseobacterium and Flavobacterium species and methods for reliable susceptibility testing. . Antimicrob Agents Chemother 41, 27382741.[PubMed]
    [Google Scholar]
  11. Hansen T. A., Imhoff J. F. ( 1985 ). Rhodobacter veldkampii, a new species of phototrophic purple nonsulfur bacteria. . Int J Syst Evol Microbiol 35, 115116.
    [Google Scholar]
  12. Helsel L. O., Hollis D., Steigerwalt A. G., Morey R. E., Jordan J., Aye T., Radosevic J., Jannat-Khah D., Thiry D. et al. ( 2007 ). Identification of “Haematobacter,” a new genus of aerobic Gram-negative rods isolated from clinical specimens, and reclassification of Rhodobacter massiliensis as “Haematobacter massiliensis comb. nov.”. . J Clin Microbiol 45, 12381243. [View Article] [PubMed]
    [Google Scholar]
  13. Huss V. A., Festl H., Schleifer K. H. ( 1983 ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4, 184192. [View Article] [PubMed]
    [Google Scholar]
  14. Jiang L. J., Xu H. X., Shao Z. Z., Long M. N. ( 2014 ). Defluviimonas indica sp. nov., a marine bacterium isolated from a deep-sea hydrothermal vent environment. . Int J Syst Evol Microbiol 64, 20842088. [View Article] [PubMed]
    [Google Scholar]
  15. Jung Y. T., Park S., Lee J. S., Yoon J. H. ( 2014 ). Defluviimonas aquaemixtae sp. nov., isolated from the junction between a freshwater spring and the ocean. . Int J Syst Evol Microbiol 64, 41914197. [View Article] [PubMed]
    [Google Scholar]
  16. Kates M. ( 1986 ). Techniques of lipidology: isolation, analysis, and identification of lipids. . In Laboratory Techniques in Biochemistry and Molecular Biology, , 2nd edn., pp. 100110. Edited by R. H. Bubon, P. H. van Knippenberg. . Amsterdam:: Elsevier;.
    [Google Scholar]
  17. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  18. Komagata K., Suzuki K. ( 1987 ). Lipid and cell wall analysis in bacterial systematics. . Methods Microbiol 19, 161207. [View Article]
    [Google Scholar]
  19. Mandel M., Igambi L., Bergendahl J., Dodson M. L. Jr, Scheltgen E. ( 1970 ). Correlation of melting temperature and cesium chloride buoyant density of bacterial deoxyribonucleic acid. . J Bacteriol 101, 333338.[PubMed]
    [Google Scholar]
  20. Marmur J. ( 1961 ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3, 208218. [View Article]
    [Google Scholar]
  21. Math R. K., Jin H. M., Jeong S. H., Jeon C. O. ( 2013 ). Defluviimonas aestuarii sp. nov., a marine bacterium isolated from a tidal flat, and emended description of the genus Defluviimonas Foesel et al. 2011. . Int J Syst Evol Microbiol 63, 28952900. [View Article] [PubMed]
    [Google Scholar]
  22. Rzhetsky A., Nei M. ( 1993 ). Theoretical foundation of the minimum-evolution method of phylogenetic inference. . Mol Biol Evol 10, 10731095.[PubMed]
    [Google Scholar]
  23. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  24. Sun J. Q., Xu L., Zhang Z., Li Y., Tang Y. Q., Wu X. L. ( 2014 ). Diverse bacteria isolated from microtherm oil-production water. . Antonie van Leeuwenhoek 105, 401411. [View Article] [PubMed]
    [Google Scholar]
  25. Takai K., Inoue A., Horikoshi K. ( 2002 ). Methanothermococcus okinawensis sp. nov., a thermophilic, methane-producing archaeon isolated from a Western Pacific deep-sea hydrothermal vent system. . Int J Syst Evol Microbiol 52, 10891095. [View Article] [PubMed]
    [Google Scholar]
  26. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  27. Wang D., Liu H. L., Zheng S. X., Wang G. J. ( 2014 ). Paenirhodobacter enshiensis gen. nov., sp. nov., a non-photosynthetic bacterium isolated from soil, and emended descriptions of the genera Rhodobacter and Haematobacter . . Int J Syst Evol Microbiol 64, 551558. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000181
Loading
/content/journal/ijsem/10.1099/ijs.0.000181
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error