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f sp. nov. and sp. nov., isolated from effective nodules of

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  • Authors: Yao Yao1 ,  Xin Hua Sui1 ,  Xiao Xia Zhang2 ,  En Tao Wang1,3 , Wen Xn Chen1
    • VIEW AFFILIATIONS
    • 1 1State Key Lab for Agro-Biotechnology; Ministry of Agriculture Key Lab of Soil Microbiology; College of Biological Sciences, China Agricultural University, Beijing 100193, PR China 2 2Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China 3 3Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 11340 México DF, Mexico
  • Xin Hua Sui [email protected]
  • First Published Online: 01 June 2015, International Journal of Systematic and Evolutionary Microbiology 65: 1831-1837, doi: 10.1099/ijs.0.000183
  • Subject: NEW TAXA - Proteobacteria
  • Cover date:
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  • Six slow-growing rhizobial strains isolated from effective nodules of were classified into the genus based on their 16S rRNA gene sequences. The results of multilocus sequence analysis of , and genes and 16S–23S rRNA intergenic spacer (IGS) sequence phylogeny indicated that the six strains belonged to two novel species, represented by CCBAU 53325 and CCBAU 51502, which were consistent with the results of DNA–DNA hybridization; CCBAU 53325 had 17.65–25.59 % relatedness and CCBAU 51502 had 22.69–44.58 % relatedness with five closely related type strains, USDA 76, LMG 24246, CCBAU 23086, LMG 24556 and USDA 6. In addition, analysis of phenotypic characteristics and fatty acid profiles also distinguished the test strains from defined species of . Two novel species, sp. nov., represented by the type strain CCBAU 53325 ( = HAMBI 3614 = CGMCC 1.13002 = LMG 28425), and sp. nov., represented by the type strain CCBAU 51502 ( = HAMBI 3613 = CGMCC 1.13001), are proposed to accommodate the strains.

  • The GenBank/EMBL/DDBJ accession numbers for the , , IGS, 16S rRNA, , and gene sequences of strains CCBAU 51502 and CCBAU 53325 are respectively KJ818109 and KF114576 (), KJ818108 and KF114598 (), KJ818105 and KF114622 (IGS), KJ818096 and KF114645 (16S rRNA gene), KJ818112 and KF114669 (), KJ818099 and KF114693 () and KJ818102 and KF114717 (). Accession numbers of other sequences determined in this study are detailed in Table S1.

  • Seven supplementary figures and three supplementary tables are available with the online Supplementary Material.

  • Abbreviations:
    IGS 16S–23S rRNA intergenic spacer;
    ML maximum-likelihood;
    MLSA multilocus sequence analysis.

© 2015 IUMS | Published by the Microbiology Society

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2015-06-01
2019-02-23
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