1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 65, Issue Pt_6

sp. nov., isolated from a marine sponge, and emended description of the genus Free

Abstract

A Gram-stain-negative, rod-shaped (1.2–2.1 μm × 0.8–0.9 μm), flagellated and motile marine bacterium, designated MEBiC05461, was isolated from a marine sponge inhabiting Micronesia. Strain MEBiC05461 was oxidase-negative and catalase-positive. Growth was observed at 8.0–35.6 °C (optimum 30.0 °C), at pH 5.0–9.0 (optimum pH 7.0) and with 1.5–6.0 % (w/v, optimum 2.0–2.5 %) NaCl. 16S rRNA gene sequence analysis revealed that strain MEBiC05461 showed high similarity to members of the genus (96.4–96.6 %). The predominant cellular fatty acids were C (23.9 %), summed feature 3 (Cω7 and/or Cω6; 39.7 %) and summed feature 8 (Cω7 and/or Cω6; 22.0 %). The DNA G+C content was 48.5 mol%. The major respiratory quinone was Q-8.Phosphatidylethanolamine, phosphatidylglycerol, one unidentified glycolipid, one unidentified aminolipid, one unidentified glycophospholipid and two unidentified lipids were detected as the major polar lipids. On the basis of the data from this polyphasic taxonomic study, strain MEBiC05461 should be classified as a representative of a novel species in the genus , and the name proposed is sp. nov. The type strain is MEBiC05461 ( = KCCM 42943 = JCM 16668). Emendations of the genus and species and are were also given.

  • Published Online:
© 2015 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000188
2015-06-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/6/1866.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000188&mimeType=html&fmt=ahah

References

  1. Bae S.S., Kwon K.K., Yang S.H., Lee H.S., Kim S.J., Lee J.H. ( 2007;). Flagellimonas eckloniae gen. nov., sp. nov., a mesophilic marine bacterium of the family Flavobacteriaceae, isolated from the rhizosphere of Ecklonia kurome . Int J Syst Evol Microbiol 57 1050–1054. [CrossRef]
    [Google Scholar]
  2. Becker P.T., Gillan D.C., Eeckhaut I. ( 2009;). Characterization of the bacterial community associated with body wall lesions of Tripneustes gratilla (Echinoidea) using culture-independent methods. J Invertebr Pathol 100 127–130. [CrossRef]
    [Google Scholar]
  3. Carder J.H. ( 1986;). Detection and quantitation of cellulase by Congo red staining of substrates in a cup-plate diffusion assay. Anal Biochem 153 75–79. [CrossRef]
    [Google Scholar]
  4. Collins M.D. ( 1985;). Isoprenoid quinone analysis in bacterial classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by Goodfellow M., Minikin D. E. London: Academic Press;.
     [Google Scholar]
  5. D'Ambrosio L., Ziervogel K., MacGregor B., Teske A., Arnosti C. ( 2014;). Composition and enzymatic function of particle-associated and free-living bacteria: a coastal/offshore comparison. ISME J 8 2167–2179. [CrossRef]
    [Google Scholar]
  6. Dishaw L.J., Flores-Torres J., Lax S., Gemayel K., Leigh B., Melillo D., Mueller M.G., Natale L., Zucchetti I., other authors. ( 2014;). The gut of geographically disparate Ciona intestinalis harbors a core microbiota. PLoS ONE 9 e93386. [CrossRef]
    [Google Scholar]
  7. Esteves A.I., Hardoim C.C., Xavier J.R., Gonçalves J.M., Costa R. ( 2013;). Molecular richness and biotechnological potential of bacteria cultured from Irciniidae sponges in the north-east Atlantic. FEMS Microbiol Ecol 85 519–536. [CrossRef]
    [Google Scholar]
  8. Felsenstein J. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17 368–376. [CrossRef]
    [Google Scholar]
  9. Fitch W.M. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20 406–416. [CrossRef]
    [Google Scholar]
  10. Gärtner A., Wiese J., Imhoff J.F. ( 2008;). Amphritea atlantica gen. nov., sp. nov., a gammaproteobacterium from the Logatchev hydrothermal vent field. Int J Syst Evol Microbiol 58 34–39. [CrossRef]
    [Google Scholar]
  11. Goffredi S.K., Johnson S.B., Vrijenhoek R.C. ( 2007;). Genetic diversity and potential function of microbial symbionts associated with newly discovered species of Osedax polychaete worms. Appl Environ Microbiol 73 2314–2323. [CrossRef]
    [Google Scholar]
  12. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y.S., Lee J.-H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716–721. [CrossRef]
    [Google Scholar]
  13. Kwon K.K., Lee H.-S., Yang S.H., Kim S.-J. ( 2005;). Kordiimonas gwangyangensis gen. nov., sp. nov., a marine bacterium isolated from marine sediments that forms a distinct phyletic lineage (Kordiimonadales ord. nov.) in the ‘Alphaproteobacteria’. Int J Syst Evol Microbiol 55 2033–2037. [CrossRef]
    [Google Scholar]
  14. Li H., Yu Y., Luo W., Zeng Y., Chen B. ( 2009;). Bacterial diversity in surface sediments from the Pacific Arctic Ocean. Extremophiles 13 233–246. [CrossRef]
    [Google Scholar]
  15. Minnikin D.E., O'Donnell A.G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J.H. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2 233–241. [CrossRef]
    [Google Scholar]
  16. Miyazaki M., Nogi Y., Fujiwara Y., Kawato M., Nagahama T., Kubokawa K., Horikoshi K. ( 2008;). Amphritea japonica sp. nov. and Amphritea balenae sp. nov., isolated from the sediment adjacent to sperm whale carcasses off Kagoshima, Japan. Int J Syst Evol Microbiol 58 2815–2820. [CrossRef]
    [Google Scholar]
  17. Pike R.E. ( 2013). A comprehensive analysis of the microbial diversity associated with Eunicea fusca and related Plexauridae octocorals, and the isolation of new natural products from selected, associated bacteria PhD Thesis. University of Prince Edward Island, Prince Edward Island .
     [Google Scholar]
  18. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406–425.
     [Google Scholar]
  19. Sasser M. ( 1990). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
     [Google Scholar]
  20. Séré M.G., Tortosa P., Chabanet P., Turquet J., Quod J.-P., Schleyer M.H. ( 2013;). Bacterial communities and putative pathogens associated with Porites white patch syndrome (PWPS) on three Western Indian Ocean (WIO) coral reefs. PLoS ONE 8 e83746. [CrossRef]
    [Google Scholar]
  21. Stackebrandt E., Liesack W. ( 1993;). Nucleic acids and classification. . In Handbook of New Bacterial Systematics, pp. 158–160. Edited by Goodfellow M., O'Donnell A. G. London: Academic Press;.
     [Google Scholar]
  22. Sunagawa S., DeSantis T.Z., Piceno Y.M., Brodie E.L., DeSalvo M.K., Voolstra C.R., Weil E., Andersen G.L., Medina M. ( 2009;). Bacterial diversity and White Plague Disease-associated community changes in the Caribbean coral Montastraea faveolata . ISME J 3 512–521. [CrossRef]
    [Google Scholar]
  23. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28 2731–2739. [CrossRef]
    [Google Scholar]
  24. Verna C., Ramette A., Wiklund H., Dahlgren T.G., Glover A.G., Gaill F., Dubilier N. ( 2010;). High symbiont diversity in the bone-eating worm Osedax mucofloris from shallow whale-falls in the North Atlantic. Environ Microbiol 12 2355–2370. [CrossRef]
    [Google Scholar]
  25. Weisburg W.G., Barns S.M., Pelletier D.A., Lane D.J. ( 1991;). 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173 697–703.
     [Google Scholar]
  26. Yang S.-H., Kwon K.K., Lee H.-S., Kim S.-J. ( 2006;). Shewanella spongiae sp. nov., isolated from a marine sponge. Int J Syst Evol Microbiol 56 2879–2882. [CrossRef]
    [Google Scholar]
  27. Yang S.-H., Seo H.-S., Oh H.-M., Kim S.-J., Lee J.-H., Kwon K.K. ( 2013;). Brumimicrobium mesophilum sp. nov., isolated from a tidal flat sediment, and emended descriptions of the genus Brumimicrobium Brumimicrobium glaciale . Int J Syst Evol Microbiol 63 1105–1110. [CrossRef]
    [Google Scholar]
  28. Zhang J.W. ( 2011). The diversity and biotechnological application of marine microbes producing omega-3 fatty acids PhD Thesis. University of Newcastle upon Tyne, Newcastle upon Tyne, UK .
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000188
Loading
Amphritea spongicola sp. nov., isolated from a marine sponge, and emended description of the genus Amphritea
Int J Syst Evol Microbiol 65, 1866 (2015); https://doi.org/10.1099/ijs.0.000188
/content/journal/ijsem/10.1099/ijs.0.000188
/content/journal/ijsem/10.1099/ijs.0.000188
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error