1887

Abstract

A heterotrophic, Gram-negative, aerobic bacterium, designated strain SB1, was isolated from surface water of the southern North Sea. Comparison of 16S rRNA gene sequences revealed that strain SB1 is affiliated to the genus within the marine clade (family ), with as the closest described species (98.2 % sequence similarity to the type strain). DNA–DNA hybridization indicated that SB1 represents a distinct species within this genus. On marine agar, strain SB1 formed beige, circular and convex colonies. Cells were irregular, motile rods. Growth occurred between 4 and 25 °C and was optimal at 20 °C, and at pH 7–9 (optimum pH 7.5–8.5) and NaCl concentrations between 1 and 6 % (optimum 2–4 %). The DNA G+C content of SB1 was 54.7 mol%. The fatty acids (>1 %) comprised 10 : 0 3-OH, 12 : 1 3-OH, 16 : 1ω7, 16 : 0, 18 : 2ω7,12, 18 : 1ω7, 18 : 0 and 11-methyl 18 : 1ω7. The sole respiratory lipoquinone was ubiquinone Q-10 and the polar lipid pattern indicated the presence of the phospholipids phosphatidylglycerol and phosphatidylcholine, as well as unidentified aminolipid AL1, phospholipids PL1 and PL3 and lipids L1, L2 and L4. On the basis of phylogenetic and phenotypic differences, strain SB1 represents a novel species in the genus , for which we propose the name sp. nov. The type strain is SB1 ( = DSM 26878 = LMG 27946). Furthermore, our results suggest the reclassification of as the type species of a new genus, gen. nov., as comb. nov. (type strain SSK2-1 = KCTC 32535 = CECT 8397). Finally, emended descriptions of the genus and its species and are also provided.

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2015-06-01
2024-03-28
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References

  1. Allgaier M., Uphoff H., Felske A., Wagner-Döbler I. ( 2003;). Aerobic anoxygenic photosynthesis in Roseobacter clade bacteria from diverse marine habitats. Appl Environ Microbiol 69 50515059 [View Article] [PubMed].
    [Google Scholar]
  2. Breider S., Scheuner C., Schumann P., Fiebig A., Petersen J.A., Pradella S., Klenk H.-P., Brinkhoff T., Göker M. ( 2014;). Genome-scale data suggest reclassifications in the Leisingera-Phaeobacter cluster including proposals for Sedimentitalea gen. nov. and Pseudophaeobacter gen. nov.. Front Microbiol 5 416 [View Article] [PubMed].
    [Google Scholar]
  3. Brinkhoff T., Giebel H.-A., Simon M. ( 2008;). Diversity, ecology, and genomics of the Roseobacter clade: a short overview. Arch Microbiol 189 531539 [View Article] [PubMed].
    [Google Scholar]
  4. Buchan A., González J.M., Moran M.A. ( 2005;). Overview of the marine Roseobacter lineage. Appl Environ Microbiol 71 56655677 [View Article] [PubMed].
    [Google Scholar]
  5. Cashion P., Holder-Franklin M.A., McCully J., Franklin M. ( 1977;). A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81 461466 [View Article] [PubMed].
    [Google Scholar]
  6. De Ley J., Cattoir H., Reynaerts A. ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12 133142 [View Article] [PubMed].
    [Google Scholar]
  7. Eilers H., Pernthaler J., Peplies J., Glöckner F.O., Gerdts G., Amann R. ( 2001;). Isolation of novel pelagic bacteria from the German bight and their seasonal contributions to surface picoplankton. Appl Environ Microbiol 67 51345142 [View Article] [PubMed].
    [Google Scholar]
  8. Giebel H.-A., Kalhoefer D., Lemke A., Thole S., Gahl-Janssen R., Simon M., Brinkhoff T. ( 2011;). Distribution of Roseobacter RCA and SAR11 lineages in the North Sea and characteristics of an abundant RCA isolate. ISME J 5 819 [View Article] [PubMed].
    [Google Scholar]
  9. Giebel H.-A., Kalhoefer D., Gahl-Janssen R., Choo Y.-J., Lee K., Cho J.-C., Tindall B.J., Rhiel E., Beardsley C., other authors. ( 2013;). Planktomarina temperata gen. nov., sp. nov., belonging to the globally distributed RCA cluster of the marine Roseobacter clade, isolated from the German Wadden Sea. Int J Syst Evol Microbiol 63 42074217 [View Article] [PubMed].
    [Google Scholar]
  10. Gosink J., Herwig R., Staley J. ( 1997;). Octadecabacter arcticus gen. nov., sp. nov., and O. antarcticus, sp. nov., nonpigmented, psychrophilic gas vacuolate bacteria from polar sea ice and water. Syst Appl Microbiol 20 356365 [View Article].
    [Google Scholar]
  11. Gregersen T. ( 1978;). Rapid method for distinction of Gram-negative from Gram-positive Bacteria. Appl Microbiol Biotechnol 5 123127 [View Article].
    [Google Scholar]
  12. Hahnke S., Tindall B.J., Schumann P., Sperling M., Brinkhoff T., Simon M. ( 2012;). Planktotalea frisia gen. nov., sp. nov., isolated from the southern North Sea. Int J Syst Evol Microbiol 62 16191624 [View Article] [PubMed].
    [Google Scholar]
  13. Huss V.A.R., Festl H., Schleifer K.H. ( 1983;). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4 184192 [View Article] [PubMed].
    [Google Scholar]
  14. Labrenz M., Collins M.D., Lawson P.A., Tindall B.J., Braker G., Hirsch P. ( 1998;). Antarctobacter heliothermus gen. nov., sp. nov., a budding bacterium from hypersaline and heliothermal Ekho Lake. Int J Syst Bacteriol 48 13631372 [View Article] [PubMed].
    [Google Scholar]
  15. Lane D.J. ( 1991;). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115175. Edited by Stackebrandt E., Goodfellow M. New York: Wiley;.
    [Google Scholar]
  16. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar Buchner,A., Lai T., Steppi S., other authors. ( 2004;). arb: a software environment for sequence data. Nucleic Acids Res . 32 13631371 [View Article] [PubMed].
    [Google Scholar]
  17. Martens T., Heidorn T., Pukall R., Simon M., Tindall B.J., Brinkhoff T. ( 2006;). Reclassification of Roseobacter gallaeciensis Ruiz-Ponte et al. 1998 as Phaeobacter gallaeciensis gen. nov., comb. nov., description of Phaeobacter inhibens sp. nov., reclassification of Ruegeria algicola (Lafay et al. 1995) Uchino et al. 1999 as Marinovum algicola gen. nov., comb. nov., and emended descriptions of the genera Roseobacter Ruegeria Leisingera . Int J Syst Evol Microbiol 56 12931304 [View Article] [PubMed].
    [Google Scholar]
  18. Mesbah M., Premachandran U., Whitman W.B. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. Int J Syst Bacteriol 39 159167 [View Article].
    [Google Scholar]
  19. Park S., Yoon J.-H. ( 2014;). Octadecabacter jejudonensis sp. nov., isolated from the junction between the ocean and a freshwater spring and emended description of the genus Octadecabacter . Int J Syst Evol Microbiol 64 719724 [View Article] [PubMed].
    [Google Scholar]
  20. Pujalte M.J., Lucena T., Ruvira M.A., Arahal D.R., Macián M.C. ( 2014;). The family Rhodobacteraceae . . In The Prokaryotes 8 , 4th edn.., pp. 439512. Edited by Rosenberg E., DeLong E. F., Lory S., Stackebrandt E., Thompson F. Berlin: Springer; [View Article] .
    [Google Scholar]
  21. Shiba T. ( 1991;). Roseobacter litoralis gen. nov., sp. nov., and Roseobacter denitrificans sp. nov., aerobic pink-pigmented bacteria which contain bacteriochlorophyll a . Syst Appl Microbiol 14 140145 [View Article].
    [Google Scholar]
  22. Smibert R.M., Krieg N.R. ( 1994;). Phenotypic characterization. . In In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  23. Stackebrandt E., Goebel B.M. ( 1994;). A place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44 846849 [View Article].
    [Google Scholar]
  24. Strömpl C., Tindall B.J., Jarvis G.N., Lünsdorf H., Moore E.R.B., Hippe H. ( 1999;). A re-evaluation of the taxonomy of the genus Anaerovibrio, with the reclassification of Anaerovibrio glycerini as Anaerosinus glycerini gen. nov., comb. nov., and Anaerovibrio burkinabensis as Anaeroarcus burkinensis [corrig.] gen. nov., comb. nov. Int J Syst Bacteriol 49 18611872 [View Article] [PubMed].
    [Google Scholar]
  25. Tamaoka J., Komagata K. ( 1984;). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25 125128 [View Article].
    [Google Scholar]
  26. Tindall B.J. ( 1990a;). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13 128130 [View Article].
    [Google Scholar]
  27. Tindall B.J. ( 1990b;). Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66 199202 [View Article].
    [Google Scholar]
  28. Tindall B.J. ( 1996;). Respiratory lipoquinones as biomarkers. . In Molecular Microbial Ecology Manua section 4.1.5, suppl. 1 . Edited by Akkermans A., de Bruijn F., van Elsas D. Dordrecht: Kluwer;.
    [Google Scholar]
  29. Vollmers J., Voget S., Dietrich S., Gollnow K., Smits M., Meyer K., Brinkhoff T., Simon M., Daniel R. ( 2013;). Poles apart: Arctic and Antarctic Octadecabacter strains share high genome plasticity and a new type of xanthorhodopsin. PLoS One 8 e63422 [View Article] [PubMed].
    [Google Scholar]
  30. Wayne L.G., Brenner D.J., Colwell R.R., Grimont P.A.D., Kandler O., Krichevsky M.I., Moore L.H., Moore W.E.C., Murray R.G.E., other authors. ( 1987;). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37 463464 [View Article].
    [Google Scholar]
  31. Yutin N., Suzuki M.T., Béjà O. ( 2005;). Novel primers reveal wider diversity among marine aerobic anoxygenic phototrophs. Appl Environ Microbiol 71 89588962 [View Article] [PubMed].
    [Google Scholar]
  32. Zech H., Thole S., Schreiber K., Kalhöfer D., Voget S., Brinkhoff T., Simon M., Schomburg D., Rabus R. ( 2009;). Growth phase-dependent global protein and metabolite profiles of Phaeobacter gallaeciensis strain DSM 17395, a member of the marine Roseobacter-clade. Proteomics 9 36773697 [View Article] [PubMed].
    [Google Scholar]
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