1887

Abstract

A novel, strictly anaerobic, hydrogenotrophic methanogen, strain E09F.3, was isolated from a commercial biogas plant in Germany. Cells of E09F.3 were Gram-stain-negative, non-motile, slightly curved rods, long chains of which formed large aggregates consisting of intertwined bundles of chains. Cells utilized H+CO and, to a lesser extent, formate as substrates for growth and methanogenesis. The optimal growth temperature was around 40 °C; maximum growth rate was obtained at pH around 7.0 with approximately 6.8 mM NaCl. The DNA G+C content of strain E09F.3 was 39.1 mol%. Phylogenetic analyses based on 16S rRNA and gene sequences placed strain E09F.3 within the genus . On the basis of 16S rRNA gene sequence similarity, strain E09F.3 was closely related to C but morphological, physiological and genomic characteristics indicated that strain E09F.3 represents a novel species. The name sp. nov. is proposed for this novel species, with strain E09F.3 ( = DSM 29428 = JCM 30569) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000210
2015-06-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/6/1975.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000210&mimeType=html&fmt=ahah

References

  1. Balch W.E., Wolfe R.S. ( 1976;). New approach to the cultivation of methanogenic bacteria: 2-mercaptoethanesulfonic acid (HS-CoM)-dependent growth of Methanobacterium ruminantium in a pressurized atmosphere. Appl Environ Microbiol 32 781791 [PubMed].
    [Google Scholar]
  2. Blotevogel K.-H., Fischer U. ( 1985;). Isolation and characterization of a new thermophilic and autotrophic methane producing bacterium: Methanobacterium thermoaggregans spec. nov. Arch Microbiol 142 218222 10.1007/BF00693393 . [CrossRef]
    [Google Scholar]
  3. Boone D.R. ( 2001;). Genus I. Methanobacterium . . In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1, pp. 215218. Edited by Boone D. R., Castenholz R. W., Garrity G. M. New York: Springer; 10.1007/978-0-387-21609-6 .
    [Google Scholar]
  4. Boone D.R., Whitman W.B. ( 1988;). Proposal of minimal standards for describing new taxa of methanogenic bacteria. Int J Syst Bacteriol 38 212219 doi: 10.1099/00207713-38-2-212 . [CrossRef]
    [Google Scholar]
  5. Borrel G., Joblin K., Guedon A., Colombet J., Tardy V., Lehours A.C., Fonty G. ( 2012;). Methanobacterium lacus sp. nov., isolated from the profundal sediment of a freshwater meromictic lake. Int J Syst Evol Microbiol 62 16251629 10.1099/ijs.0.034538-0 [PubMed]. [CrossRef]
    [Google Scholar]
  6. Bryant M.P., Tzeng S.F., Robinson I.M., Joyner A.E. Jr ( 1971;). Nutrient requirements of methanogenic bacteria. Adv Chem Ser 105 2340. [CrossRef]
    [Google Scholar]
  7. Bryant M.P., Boone D.R. ( 1987;). Isolation and characterization of Methanobacterium formicicum MF. Int J Syst Bacteriol 37 171. [CrossRef]
    [Google Scholar]
  8. Cadillo-Quiroz H., Bräuer S.L., Goodson N., Yavitt J.B., Zinder S.H. ( 2014;). Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands. Int J Syst Evol Microbiol 64 14731480 10.1099/ijs.0.059964-0 [PubMed]. [CrossRef]
    [Google Scholar]
  9. Cuzin N., Ouattara A.S., Labat M., Garcia J.L. ( 2001;). Methanobacterium congolense sp. nov., from a methanogenic fermentation of cassava peel. Int J Syst Evol Microbiol 51 489493 [PubMed].
    [Google Scholar]
  10. De Ley J., Cattoir H., Reynaerts A. ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12 133142 10.1111/j.1432-1033.1970.tb00830.x [PubMed]. [CrossRef]
    [Google Scholar]
  11. DeLong E.F. ( 1992;). Archaea in coastal marine environments. Proc Natl Acad Sci U S A 89 56855689 10.1073/pnas.89.12.5685 [PubMed]. [CrossRef]
    [Google Scholar]
  12. Doddema H.J., Vogels G.D. ( 1978;). Improved identification of methanogenic bacteria by fluorescence microscopy. Appl Environ Microbiol 36 752754 [PubMed].
    [Google Scholar]
  13. Gerhardt P., Murray R.G.E., Wood W.A., Krieg N.R.(editors). ( 1994). Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  14. Godsy E.M. ( 1980;). Isolation of Methanobacterium bryantii from a deep aquifer by using a novel broth-antibiotic disk method. Appl Environ Microbiol 39 10741075 [PubMed].
    [Google Scholar]
  15. Huß V.A.R., Festl H., Schleifer K.H. ( 1983;). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4 184192 10.1016/S0723-2020(83)80048-4 [PubMed]. [CrossRef]
    [Google Scholar]
  16. Joulian C., Patel B.K., Ollivier B., Garcia J.L., Roger P.A. ( 2000;). Methanobacterium oryzae sp. nov., a novel methanogenic rod isolated from a Philippines ricefield. Int J Syst Evol Microbiol 50 525528 10.1099/00207713-50-2-525 [PubMed]. [CrossRef]
    [Google Scholar]
  17. Klocke M., Mähnert P., Mundt K., Souidi K., Linke B. ( 2007;). Microbial community analysis of a biogas-producing completely stirred tank reactor fed continuously with fodder beet silage as mono-substrate. Syst Appl Microbiol 30 139151 10.1016/j.syapm.2006.03.007 [PubMed]. [CrossRef]
    [Google Scholar]
  18. Krakat N., Schmidt S., Scherer P. ( 2010;). Mesophilic fermentation of renewable biomass: does hydraulic retention time regulate methanogen diversity?. Appl Environ Microbiol 76 63226326 10.1128/AEM.00927-10 [PubMed]. [CrossRef]
    [Google Scholar]
  19. Leclerc M., Delgènes J.P., Godon J.J. ( 2004;). Diversity of the archaeal community in 44 anaerobic digesters as determined by single strand conformation polymorphism analysis and 16S rDNA sequencing. Environ Microbiol 6 809819 10.1111/j.1462-2920.2004.00616.x [PubMed]. [CrossRef]
    [Google Scholar]
  20. Luton P.E., Wayne J.M., Sharp R.J., Riley P.W. ( 2002;). The mcrA gene as an alternative to 16S rRNA in the phylogenetic analysis of methanogen populations in landfill. Microbiology 148 35213530 [PubMed].
    [Google Scholar]
  21. Ma K., Liu X., Dong X. ( 2005;). Methanobacterium beijingense sp. nov., a novel methanogen isolated from anaerobic digesters. Int J Syst Evol Microbiol 55 325329 10.1099/ijs.0.63254-0 [PubMed]. [CrossRef]
    [Google Scholar]
  22. Mesbah M., Premachandran U., Whitman W.B. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39 159167 doi: 10.1099/00207713-39-2-159 . [CrossRef]
    [Google Scholar]
  23. Nölling J., Elfner A., Palmer J.R., Steigerwald V.J., Pihl T.D., Lake J.A., Reeve J.N. ( 1996;). Phylogeny of Methanopyrus kandleri based on methyl coenzyme M reductase operons. Int J Syst Bacteriol 46 11701173 10.1099/00207713-46-4-1170 [PubMed]. [CrossRef]
    [Google Scholar]
  24. Schirmack J., Mangelsdorf K., Ganzert L., Sand W., Hillebrand-Voiculescu A., Wagner D. ( 2014;). Methanobacterium movilense sp. nov., a hydrogenotrophic, secondary-alcohol-utilizing methanogen from the anoxic sediment of a subsurface lake. Int J Syst Evol Microbiol 64 522527 10.1099/ijs.0.057224-0 [PubMed]. [CrossRef]
    [Google Scholar]
  25. Shcherbakova V., Rivkina E., Pecheritsyna S., Laurinavichius K., Suzina N., Gilichinsky D. ( 2011;). Methanobacterium arcticum sp. nov., a methanogenic archaeon from Holocene Arctic permafrost. Int J Syst Evol Microbiol 61 144147 10.1099/ijs.0.021311-0 [PubMed]. [CrossRef]
    [Google Scholar]
  26. Simankova M.V., Kotsyurbenko O.R., Lueders T., Nozhevnikova A.N., Wagner B., Conrad R., Friedrich M.W. ( 2003;). Isolation and characterization of new strains of methanogens from cold terrestrial habitats. Syst Appl Microbiol 26 312318 10.1078/072320203322346173 [PubMed]. [CrossRef]
    [Google Scholar]
  27. Stackebrandt E., Ebers J. ( 2006;). Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33 152155.
    [Google Scholar]
  28. Stantscheff R., Kuever J., Rabenstein A., Seyfarth K., Dröge S., König H. ( 2014;). Isolation and differentiation of methanogenic Archaea from mesophilic corn-fed on-farm biogas plants with special emphasis on the genus Methanobacterium . Appl Microbiol Biotechnol 98 57195735 10.1007/s00253-014-5652-4 [PubMed]. [CrossRef]
    [Google Scholar]
  29. Tamaoka J., Komagata K. ( 1984;). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25 125128 doi: 10.1111/j.1574-6968.1984.tb01388.x . [CrossRef]
    [Google Scholar]
  30. Wasserfallen A., Nölling J., Pfister P., Reeve J., Conway de Macario E. ( 2000;). Phylogenetic analysis of 18 thermophilic Methanobacterium isolates supports the proposals to create a new genus, Methanothermobacter gen. nov., and to reclassify several isolates in three species, Methanothermobacter thermautotrophicus comb. nov., Methanothermobacter wolfeii comb. nov., and Methanothermobacter marburgensis sp. nov. Int J Syst Evol Microbiol 50 4353 10.1099/00207713-50-1-43 [PubMed]. [CrossRef]
    [Google Scholar]
  31. Wayne L.G., Brenner D.J., Colwell R.R., Grimont P.A.D., Kandler O., Krichevsky M.I., Moore L.H., Moore W.E.C., Murray R.G.E., other authors. ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37 463464 doi: 10.1099/00207713-37-4-463 . [CrossRef]
    [Google Scholar]
  32. Weisburg W.G., Barns S.M., Pelletier D.A., Lane D.J. ( 1991;). 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173 697703 [PubMed].
    [Google Scholar]
  33. Whitman W.B., Bowen T.L., Boone D.R. ( 2006;). The methanogenic bacteria. . In The Prokaryotes, 3rd edn, vol. 3, pp. 165207. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K.-H., Stackebrandt E. New York: Springer; doi: 10.1007/0-387-30743-5_9 . [CrossRef]
    [Google Scholar]
  34. Wolin E.A., Wolin M.J., Wolfe R.S. ( 1963;). Formation of methane by bacterial extracts. J Biol Chem 238 28822886 [PubMed].
    [Google Scholar]
  35. Zhang C., Yuan Q., Lu Y. ( 2014;). Inhibitory effects of ammonia on methanogen mcrA transcripts in anaerobic digester sludge. FEMS Microbiol Ecol 87 368377 10.1111/1574-6941.12229 [PubMed]. [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000210
Loading
/content/journal/ijsem/10.1099/ijs.0.000210
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error