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f sp. nov., isolated from a lead–zinc tailing

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  • Authors: Ling Chen1 ,  Lu Wang1 , Xia-Fang Sheng1
    • VIEW AFFILIATIONS
    • 1 Key Laboratory of Agricultural Environment Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China
  • Xia-Fang Sheng [email protected]
  • First Published Online: 01 July 2015, International Journal of Systematic and Evolutionary Microbiology 65: 2161-2166, doi: 10.1099/ijs.0.000232
  • Subject: NEW TAXA - Firmicutes and Related Organisms
  • Cover date:
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  • A novel bacterial strain, S1-9, was isolated from a lead–zinc tailing in Nanjing, Jiangsu Province, China. Cells of strain S1-9 were Gram-stain-negative, ellipsoidal endospore-forming, aerobic rods and motile by means of peritrichous flagella. On the basis of 16S rRNA gene sequence analysis, strain S1-9 was shown to belong to the genus and the closest phylogenetic relatives were DSM 5162 (96.8 % similarity), NRRL NRS-666 (96.5 %) and MB 1871 (95.4 %). The predominant menaquinone was MK-7. The major cellular fatty acids were anteiso-C and iso-C. The polar lipid profile contained phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, two unknown phospholipids and two unknown lipids. The total DNA G+C content of strain S1-9 was 49.9 mol%. Based on the low levels of DNA–DNA relatedness (ranging from 21.8 to 48.4 %) to the type strains of the above species of the genus and unique phenotypic characteristics, strain S1-9 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is S1-9 ( = CCTCC AB 2014290 = JCM 30613).

  • The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain S1-9 is KP027419.

  • Three supplementary tables and six supplementary figures are available with the online Supplementary Material.

© 2015 IUMS | Published by the Microbiology Society

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