RT Journal Article SR Electronic(1) A1 Niu, Lili A1 Tang, Tianyi A1 Song, Lei A1 Xiong, Mengjie A1 Tian, Jianqing A1 Zhang, Kegui A1 Hu, Xing A1 Zhu, DaochenYR 2015 T1 Pullulanibacillus pueri sp. nov., isolated from Pu'er tea JF International Journal of Systematic and Evolutionary Microbiology, VO 65 IS Pt_7 SP 2167 OP 2171 DO https://doi.org/10.1099/ijs.0.000239 PB Microbiology Society, SN 1466-5034, AB A novel Gram-stain-positive, aerobic, endospore-forming, rod-shaped bacterial strain YN3T was isolated from ripened Pu'er tea. Phylogenetic analysis of 16S rRNA gene sequences showed that the strain belonged to the family Sporolactobacillaceae and was closely related to Pullulanibacillus naganoensis DSM 10191T (95.8 % 16S rRNA gene sequence similarity) and Pullulanibacillus uraniitolerans DSM 19429T (95.4 %). Growth of the strain was observed at 20–50 °C (optimum 30–37 °C), at pH 4.0–8.0 (optimum pH 5.0–6.0). The strain had a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid. The predominant menaquinone was menaquinone-7 (MK-7). The major fatty acids were anteiso-C15:0, anteiso-C17:0 and C18:1ω7c. The DNA G+C content of strain YN3T was 38.7 mol%. Strain YN3T could be differentiated from recognized species of the genus Pullulanibacillus based on phenotypic characteristics, chemotaxonomic differences, phylogenetic analysis and DNA–DNA hybridization data. On the basis of polyphasic evidence from this study, Pullulanibacillus pueri sp. nov., is proposed, with strain YN3T ( = CGMCC 1.12777T = JCM 30075T) as the type strain., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.000239