1887

Abstract

A Gram-staining-negative, non-motile, catalase- and oxidase-positive strain, designated MS7, was isolated from freshwater of a river near Trabzon, Turkey. Its taxonomy was investigated using a polyphasic approach. The strain grew optimally at 28 °C and pH 7.5 and in the presence of 2.0 % NaCl. 16S rRNA gene sequence analysis revealed that the strain belonged to the genus ; strain MS7 showed highest sequence similarity to the type strains of (97.3 %), Algoriphagus terrigena (96.8 %), (96.2 %), (96.1 %) and (95.8 %). The major fatty acids of strain MS7 were iso-C (30.14 %) and summed future 9 (10-methyl C and/or iso-C 9 18.75 %). Polar lipid analysis revealed phosphatidylethanolamine, a variety of unidentified lipids, an unidentified aminophospholipid, an unidentified phospholipid and an unidentified aminolipid. The major isoprenoid quinone was MK-7.The DNA G+C content of MS7 was 41.6 mol%, a value consistent with that of members of the genus The level of DNA–DNA relatedness between strain MS7 and LMG 22694 was 41 %, which is clearly below the 70 % threshold accepted for species delineation. Thus, our results support the placement of strain MS7 within a separate and previously unrecognized species. On the basis of these data, the strain is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MS7 ( = NCCB 100372 = LMG 26290). An emended description of is also provided.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000246
2015-07-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/7/2234.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000246&mimeType=html&fmt=ahah

References

  1. Alegado R.A., Grabenstatter J.D., Zuzow R., Morris A., Huang S.Y., Summons R.E., King N. ( 2013;). Algoriphagus machipongonensis sp. nov., co-isolated with a colonial choanoflagellate. Int J Syst Evol Microbiol 63 163168 [View Article] [PubMed].
    [Google Scholar]
  2. Atlas R.M. ( 1993;). Handbook of Microbiological Media. Edited by Parks L. C. Boca Raton, FL: :  CRC Press;.
    [Google Scholar]
  3. Beffa T., Blanc M., Lyon P.F., Vogt G., Marchiani M., Fischer J.L., Aragno M. ( 1996;). Isolation of Thermus strains from hot composts (60 to 80 °C). Appl Environ Microbiol 62 17231727 [PubMed].
    [Google Scholar]
  4. Belduz A.O., Dulger S., Demirbag˘ Z. ( 2003;). Anoxybacillus gonensis sp. nov., a moderately thermophilic, xylose-utilizing, endospore-forming bacterium. Int J Syst Evol Microbiol 53 13151320 [View Article] [PubMed].
    [Google Scholar]
  5. Bowman J.P., Nichols C.M., Gibson J.A.E. ( 2003;). Algoriphagus ratkowskyi gen. nov., sp. nov., Brumimicrobium glaciale gen. nov., sp. nov., Cryomorpha ignava gen. nov., sp. nov. and Crocinitomix catalasitica gen. nov., sp. nov., novel flavobacteria isolated from various polar habitats. Int J Syst Evol Microbiol 53 13431355 [View Article] [PubMed].
    [Google Scholar]
  6. Brosius J., Palmer M.L., Kennedy P.J., Noller H.F. ( 1978;). Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . Proc Natl Acad Sci U S A 75 48014805 [View Article] [PubMed].
    [Google Scholar]
  7. De Ley J, Cattoir H., Reynaerts A. ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12 133142 [View Article] [PubMed].
    [Google Scholar]
  8. Dussault H.P. ( 1955;). An improved technique for staining red halophilic bacteria. J Bacteriol 70 484485 [PubMed].
    [Google Scholar]
  9. Felsenstein J. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39 783791 [View Article].
    [Google Scholar]
  10. Fitch W.M. ( 1971;). Toward defining the course of evolution :  minimum change for a specific tree topology. Syst Zool 20 406416 [View Article].
    [Google Scholar]
  11. Hall T.A. ( 1999;). BioEdit :  a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41 9598.
    [Google Scholar]
  12. Huß V.A.R., Festl H., Schleifer K.H. ( 1983;). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4 184192 [View Article] [PubMed].
    [Google Scholar]
  13. Kim O.S., Cho Y.J., Lee K., Yoon S.H., Kim M., Na H., Park S.C., Jeon Y.S., Lee J.H., Yi H., Won S.J. ( 2012;). Introducing EzTaxon: a prokaryotic 16S rRNA Gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716721. [CrossRef]
    [Google Scholar]
  14. Kim H., Joung Y., Joh K. ( 2014;). Algoriphagus taeanensis sp. nov., isolated from a tidal flat, and emended description of Algoriphagus hitonicola. Int J Syst Evol Microbiol 64 2126 [View Article] [PubMed].
    [Google Scholar]
  15. Kimura M. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16 111120 [View Article] [PubMed].
    [Google Scholar]
  16. Kristjansson J.K., Alfredsson G.A. ( 1983;). Distribution of Thermus spp. in Icelandic hot springs and a thermal gradient. Appl Environ Microbiol 45 17851789 [PubMed].
    [Google Scholar]
  17. Kuykendall L.D., Roy M.A., O'Neill J.J., Devine T.E. ( 1988;). Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradirhyzobium japonicum . Int J Syst Bacteriol 38 358361 [View Article].
    [Google Scholar]
  18. Mandel M., Marmur J. ( 1968;). Use of ultraviolet absorbance-temperature profile for determination of the guanine plus cytosine content of DNA. Methods Enzymol 12B 195206 [View Article].
    [Google Scholar]
  19. Marmur J. ( 1961;). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3 208218 [View Article].
    [Google Scholar]
  20. Miller L.T. ( 1982;). Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol 16 584586 [PubMed].
    [Google Scholar]
  21. Nedashkovskaya O.I., Kim S.B., Kwon K.K., Shin D.S., Luo X., Kim S.J., Mikhailov V.V. ( 2007;). Proposal of Algoriphagus vanfongensis sp. nov., transfer of members of the genera Hongiella Yi and Chun 2004 emend. Nedashkovskaya et al. 2004 and Chimaereicella Tiago et al. 2006 to the genus Algoriphagus, and emended description of the genus Algoriphagus Bowman et al. 2003 emend. Nedashkovskaya et al. 2004. Int J Syst Evol Microbiol 57 19881994. [CrossRef]
    [Google Scholar]
  22. Oh K.H., Kang S.J., Lee S.Y., Park S., Oh T.K., Yoon J.H. ( 2012;). Algoriphagus namhaensis sp. nov., isolated from seawater. Int J Syst Evol Microbiol 62 575579 [View Article] [PubMed].
    [Google Scholar]
  23. Powers E.M. ( 1995;). Efficacy of the Ryu nonstaining KOH technique for rapidly determining Gram reactions of food-borne and waterborne bacteria and yeasts. Appl Environ Microbiol 61 37563758 [PubMed].
    [Google Scholar]
  24. Saitou N., Nei M. ( 1987;). The neighbor-joining method :  a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406425 [PubMed].
    [Google Scholar]
  25. Smibert R.M., Krieg N.R. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: :  American Society for Microbiology;.
    [Google Scholar]
  26. Stackebrandt E., Goebel B.M. ( 1994;). Taxonomic note :  a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44 846849 [View Article].
    [Google Scholar]
  27. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011;). mega5 :  molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28 27312739 [View Article] [PubMed].
    [Google Scholar]
  28. Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., Higgins D.G. ( 1997;). The clustal_x windows interface :  flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25 48764882 [View Article] [PubMed].
    [Google Scholar]
  29. Tindall B.J. ( 1990a;). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13 128130 [View Article].
    [Google Scholar]
  30. Tindall B.J. ( 1990b;). Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 66 199202 [View Article].
    [Google Scholar]
  31. Tindall B.J., Sikorski J., Smibert R.M., Krieg N.R. ( 2007;). Phenotypic characterization and the principles of comparative systematics. . In Methods for General and Molecular Microbiology , 3rd edn.., pp. 330393. Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt T. M., Snyder L. R. Washington, DC: :  American Society for Microbiology; [View Article].
    [Google Scholar]
  32. Wayne L.G., Brenner D.J., Colwell R.R., Grimont P.A.D., Kandler O., Krichevsky M.I., Moore L.H., Moore W.E.C., Murray R.G.E., other authors. ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37 463464 [View Article].
    [Google Scholar]
  33. Yang C., Li Y., Guo Q., Lai Q., Zheng T., Tian Y. ( 2013;). Algoriphagus zhangzhouensis sp. nov., isolated from mangrove sediment. Int J Syst Evol Microbiol 63 16211626 [View Article] [PubMed].
    [Google Scholar]
  34. Yoon J.H., Lee M.H., Kang S.J., Oh T.K. ( 2006;). Algoriphagus terrigena sp. nov., isolated from soil. Int J Syst Evol Microbiol 56 777780 [View Article] [PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000246
Loading
/content/journal/ijsem/10.1099/ijs.0.000246
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error