- Home
- Publications
- International Journal of Systematic and Evolutionary Microbiology
- Volume 65, Issue 7
- Article

f Roseimarinus sediminis gen. nov., sp. nov., a facultatively anaerobic bacterium isolated from coastal sediment
- Authors: Wen-Jie Wu1 , Qian-Qian Liu1 , Guan-Jun Chen1,2 , Zong-Jun Du1,2
-
- VIEW AFFILIATIONS
-
1 1College of Marine Science, Shandong University at Weihai, Weihai 264209, PR China 2 2State key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China
- Correspondence Zong-Jun Du [email protected]
- First Published Online: 01 July 2015, International Journal of Systematic and Evolutionary Microbiology 65: 2260-2264, doi: 10.1099/ijs.0.000250
- Subject: NEW TAXA - Bacteroidetes
- Cover date:




Roseimarinus sediminis gen. nov., sp. nov., a facultatively anaerobic bacterium isolated from coastal sediment, Page 1 of 1
< Previous page | Next page > /docserver/preview/fulltext/ijsem/65/7/2260_ijs000250-1.gif
-
A Gram-stain-negative, facultatively anaerobic, non-motile and pink-pigmented bacterium, designated strain HF08T, was isolated from marine sediment of the coast of Weihai, China. Cells were rod-shaped, and oxidase- and catalase-positive. The isolate grew optimally at 33 °C, at pH 7.5–8.0 and with 2–3 % (w/v) NaCl. The dominant cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0 and iso-C14 : 0. Menaquinone 7 (MK-7) was the major respiratory quinone and the DNA G+C content was 44.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolate was a member of the class Bacteroidia, and shared 88–90 % sequence similarity with the closest genera Sunxiuqinia, Prolixibacter, Draconibacterium, Mariniphaga and Meniscus. Based on the phylogenetic and phenotypic evidence presented, a novel species in a new genus of the family Prolixibacteraceae is proposed, with the name Roseimarinus sediminis gen. nov., sp. nov. The type strain of Roseimarinus sediminis is HF08T ( = KCTC 42261T = CICC 10901T).
-
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain HF08T is KP019205.
-
One supplementary figure and one supplementary table are available with the online Supplementary Material.
© 2015 IUMS | Published by the Microbiology Society
-
Chang D.-H., Lee J.-B., Lee G.-H., Rhee M.-S., Lee H., Bae K.-S., Park D.-S., Kim B.-C.. ( 2013;). Sunxiuqinia dokdonensis sp. nov., isolated from deep sub-seafloor sediment. J Microbiol 51: 741––746 [CrossRef] [PubMed].
-
da Costa M.S., Nobre M.F., Wait R.. ( 2006;). Analysis of lipids from extremophilic bacteria. Methods Microbiol 35: 127––159 [CrossRef].
-
Dong X.-Z., Cai M.-Y.. ( 2001;). Determination of biochemical characteristics. . In Manual for the Systematic Identification of General Bacteria, pp. 370––398. Edited by Dong X. -Z., Cai M. -Y.. Beijing: Science Press (in Chinese);.
-
Du Z.-J., Wang Y., Dunlap C., Rooney A.P., Chen G.-J.. ( 2014;). Draconibacterium orientale gen. nov., sp. nov., isolated from two distinct marine environments, and proposal of Draconibacteriaceae fam. nov. Int J Syst Evol Microbiol 64: 1690––1696 [CrossRef] [PubMed].
-
Felsenstein J.. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17: 368––376 [CrossRef] [PubMed].
-
Fitch W.M.. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20: 406––416 [CrossRef].
-
Hall T.A.. ( 1999;). BioEdit: a user-friendly sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symp Ser 41: 95––98.
-
Holmes D.E., Nevin K.P., Woodard T.L., Peacock A.D., Lovley D.R.. ( 2007;). Prolixibacter bellariivorans gen. nov., sp. nov., a sugar-fermenting, psychrotolerant anaerobe of the phylum Bacteroidetes, isolated from a marine-sediment fuel cell. Int J Syst Evol Microbiol 57: 701––707 [CrossRef] [PubMed].
-
Huang X.-F., Liu Y.-J., Dong J.-D., Qu L.-Y., Zhang Y.-Y., Wang F.-Z., Tian X.-P., Zhang S.. ( 2014;). Mangrovibacterium diazotrophicum gen. nov., sp. nov., a nitrogen-fixing bacteriu isolated from a mangrove sediment, and proposal of Prolixibacteraceae fam. nov. Int J Syst Evol Microbiol 64: 875––881 [CrossRef] [PubMed].
-
Iino T., Mori K., Itoh T., Kudo T., Suzuki K., Ohkuma M.. ( 2014;). Description of Mariniphaga anaerophila gen. nov., sp. nov., a facultatively aerobic marine bacterium isolated from tidal flat sediment, reclassification of the Draconibacteriaceae as a later heterotypic synonym of the Prolixibacteraceae and description of the family Marinifilaceae fam. nov. Int J Syst Evol Microbiol 64: 3660––3667 [CrossRef] [PubMed].
-
Irgens R.L.. ( 1977;). Meniscus, a new genus of aerotolerant, gas-vacuolated bacteria. Int J Syst Bacteriol 27: 38––43 [CrossRef].
-
Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y.S., Lee J.-H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716––721 [CrossRef] [PubMed].
-
Kimura M.. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16: 111––120 [CrossRef] [PubMed].
-
Kroppenstedt R.M.. ( 1982;). Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 5: 2359––2367 [CrossRef].
-
Liu Q.-Q., Wang Y., Li J., Du Z.-J., Chen G.-J.. ( 2014a;). Saccharicrinis carchari sp. nov., isolated from a shark, and emended descriptions of the genus Saccharicrinis Saccharicrinis fermentans. Int J Syst Evol Microbiol 64: 2204––2209 [CrossRef] [PubMed].
-
Liu Q.-Q., Li X.L., Rooney A.P., Du Z.-J., Chen G.-J.. ( 2014b;). Tangfeifania diversioriginum gen. nov., sp. nov., a representative of the family Draconibacteriaceae. Int J Syst Evol Microbiol 64: 3473––3477 [CrossRef] [PubMed].
-
Mesbah M., Premachandran U., Whitman W.B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39: 159––167 [CrossRef].
-
Minnikin D., O'Donnell A., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J.. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2: 233––241 [CrossRef].
-
Na H., Kim S., Moon E.Y., Chun J.. ( 2009;). Marinifilum fragile gen. nov., sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 59: 2241––2246 [CrossRef] [PubMed].
-
Qu L., Zhu F., Hong X., Gao W., Chen J., Sun X.. ( 2011;). Sunxiuqinia elliptica gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from sediment in a sea cucumber farm. Int J Syst Evol Microbiol 61: 2885––2889 [CrossRef] [PubMed].
-
Ruvira M.A., Lucena T., Pujalte M.J., Arahal D.R., Macián M.C.. ( 2013;). Marinifilum flexuosum sp. nov., a new Bacteroidetes isolated from coastal Mediterranean Sea water and emended description of the genus Marinifilum Na et al., 2009. Syst Appl Microbiol 36: 155––159 [CrossRef] [PubMed].
-
Smibert R.M., Krieg N.R.. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607––654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R.. Washington, DC: American Society for Microbiology;.
-
Takai K., Abe M., Miyazaki M., Koide O., Nunoura T., Imachi H., Inagaki F., Kobayashi T.. ( 2013;). Sunxiuqinia faeciviva sp. nov., a facultatively anaerobic organoheterotroph of the Bacteroidetes isolated from deep subseafloor sediment. Int J Syst Evol Microbiol 63: 1602––1609 [CrossRef] [PubMed].
-
Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30: 2725––2729 [CrossRef] [PubMed].
-
Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., Higgins D.G.. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25: 4876––4882 [CrossRef] [PubMed].
-
Wang Z.-J., Xie Z.-H., Wang C., Du Z.-J., Chen G.-J.. ( 2014;). Motiliproteus sediminis gen. nov., sp. nov., isolated from coastal sediment. Antonie van Leeuwenhoek 106: 615––621 [CrossRef] [PubMed].
-
Yoon J., Kasai H.. ( 2014;). Sunxiuqinia rutila sp. nov., a new member of the phylum Bacteroidetes isolated from marine sediment. J Gen Appl Microbiol 60: 28––32 [CrossRef] [PubMed].

Supplementary Data
Data loading....

Article metrics loading...

Full text loading...
Author and Article Information
-
This Journal
/content/journal/ijsem/10.1099/ijs.0.000250dcterms_title,dcterms_subject,pub_serialTitlepub_serialIdent:journal/ijsem AND -contentType:BlogPost104 -
Other Society Journals
/content/journal/ijsem/10.1099/ijs.0.000250dcterms_title,dcterms_subject-pub_serialIdent:journal/ijsem AND -contentType:BlogPost104 -
PubMed
-
Google Scholar
Figure data loading....