RT Journal Article SR Electronic(1) A1 Kim, Byoung-Jun A1 Kim, Jae-Myung A1 Kim, Bo-Ram A1 Lee, So-Young A1 Kim, GaNa A1 Jang, Yun-Ho A1 Ryoo, Soyoon A1 Jeon, Che-Ok A1 Jin, Hyun-Mi A1 Jeong, Joseph A1 Lee, Seon Ho A1 Lim, Ji-Hun A1 Kook, Yoon-Hoh A1 Kim, Bum-JoonYR 2015 T1 Mycobacterium anyangense sp. nov., a rapidly growing species isolated from blood of Korean native cattle, Hanwoo (Bos taurus coreanae) JF International Journal of Systematic and Evolutionary Microbiology, VO 65 IS Pt_7 SP 2277 OP 2285 DO https://doi.org/10.1099/ijs.0.000255 PB Microbiology Society, SN 1466-5034, AB From the whole blood of Korean native cattle, Hanwoo (Bos taurus coreanae), a previously undescribed, rapidly growing, scotochromogenic isolate of the genus Mycobacterium is reported. Its 16S rRNA gene sequence, and the sequences of three other genes (hsp65, recA and rpoB) were unique and phylogenetic analysis based on 16S rRNA gene sequence (1420 bp) placed the organism into the rapidly growing Mycobacterium group close to Mycobacterium smegmatis (98.5 % sequence similarity). However, phylogenetic analyses based on three different gene sequences (hsp65, recA and rpoB) revealed its location to be distinct from the branch of rapidly growing species. Culture and biochemical characteristics were generally similar to those of Mycobacterium fortuitum. Unique matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) MS profiles of lipids, unique fatty acid profile, unique mycolic acids profiles and a low DNA–DNA relatedness to M. fortuitum (23.6 %) and M. smegmatis (39.7 %) strongly supported the taxonomic status of this strain as a representative of a novel species of rapidly growing mycobacteria named Mycobacterium anyangense. The type strain is strain QIA-38T ( = JCM 30275T = KCTC 29443T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.000255