RT Journal Article SR Electronic(1) A1 Koeck, Daniela E. A1 Ludwig, Wolfgang A1 Wanner, Gerhard A1 Zverlov, Vladimir V. A1 Liebl, Wolfgang A1 Schwarz, Wolfgang H.YR 2015 T1 Herbinix hemicellulosilytica gen. nov., sp. nov., a thermophilic cellulose-degrading bacterium isolated from a thermophilic biogas reactor JF International Journal of Systematic and Evolutionary Microbiology, VO 65 IS Pt_8 SP 2365 OP 2371 DO https://doi.org/10.1099/ijs.0.000264 PB Microbiology Society, SN 1466-5034, AB Phenotypic and phylogenetic studies were performed on new isolates of a novel Gram-stain-positive, anaerobic, non-sporulating, rod-shaped bacterium isolated from a thermophilic biogas plant. The novel organisms were able to degrade crystalline cellulose. 16S rRNA gene comparative sequence analysis demonstrated that the isolates formed a hitherto unknown subline within the family Lachnospiraceae. As a representative of the whole group of isolates, strain T3/55T was further characterized. The closest relative of T3/55T among the taxa with validly published names is Mobilitalea sibirica, sharing 93.9 % 16S rRNA gene sequence similarity. Strain T3/55T was catalase-negative, indole-negative, and produced acetate, ethanol and propionic acid as major end products from cellulose metabolism. The major cellular fatty acids (>1 %) were 16 : 0 dimethyl acetal, 16 : 0 fatty acid methyl ester and 16 : 0 aldehyde. The DNA G+C content was 36.6 mol%. A novel genus and species, Herbinix hemicellulosilytica gen. nov., sp. nov., is proposed based on phylogenetic analysis and physiological properties of the novel isolate. Strain T3/55T ( = DSM 29228T = CECT 8801T), represents the type strain of Herbinix hemicellulosilytica gen. nov., sp. nov., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.000264