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f sp. nov., isolated from soil

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  • Authors: Hina Singh1​ ,3 ,  KyungHwa Won1​ ,3 ,  Hien T. T. Ngo1​ ,  Juan Du1​ ,  MooChang Kook2​ , Tae-Hoo Yi1​
    • VIEW AFFILIATIONS
    • 1​ 1​ Department of Oriental Medicine Biotechnology, College of Life Science, Kyung Hee University, 1 Seocheon, Kihung Yongin, Gyeonggi 446-701, Republic of Korea 2​ 2​ Department of Marine Biotechnology, Anyang University, Incheon 417-833, Republic of Korea
  • Tae-Hoo Yi [email protected]
  • First Published Online: 01 August 2015, International Journal of Systematic and Evolutionary Microbiology 65: 2351-2356, doi: 10.1099/ijs.0.000265
  • Subject: NEW TAXA - Actinobacteria
  • Cover date:
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  • A Gram-stain-positive, non-motile, coccus-shaped bacterium, strain THG-a14, was isolated from soil of Gyeyang mountain in Incheon, Republic of Korea. The isolate grew optimally at 28 °C, at pH 6.5–7.5 and with 0–3 % (w/v) NaCl. Based on 16S rRNA gene sequence comparisons, strain THG-a14 was closely related to 5516T-20 (97.7 %), BXN5-13 (97.6 %), ‘P. ’ L1b-b9 (97.4 %) and MSL-03 (97.2 %). The DNA G+C content of strain THG-a14 was 71.6 mol%. In DNA–DNA hybridization, the DNA–DNA relatedness between strain THG-a14 and its closest phylogenetically neighbours was below 50.0 %. Strain THG-a14 was characterized chemotaxonomically as having -diaminopimelic acid in the cell-wall peptidoglycan. Strain THG-a14 contained glucose and ribose as whole-cell-wall sugars and menaquinone MK-8(H) as the major isoprenoid quinone. Polar lipids in strain THG-a14 consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphoaminoglycolipids, unidentified phospholipids and unidentified lipids. The major fatty acids were iso-C , iso-C and C ω8. On the basis of our polyphasic taxonomy study, strain THG-a14 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is THG-a14 ( = KACC 17892 = JCM 19837).

  • †These authors contributed equally to this work.

  • The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain THG-a14 is KF999706.

  • Four supplementary figures and a supplementary table are available with the online Supplementary Material.

© 2015 IUMS | Published by the Microbiology Society

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