1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 65, Issue Pt_8

sp. nov., isolated from a soil sample of a mud volcano Free

Abstract

A novel Gram-stain-positive, coccoid, non-motile bacterium, designated strain AMV4, was isolated from a soil sample collected from a mud volcano located in the Andaman Islands, India. The colony was pale orange. Strain AMV4 was positive for oxidase, aesculinase, lysine decarboxylase and ornithine decarboxylase activities and negative for amylase, catalase, cellulase, protease, urease and lipase activities. 16S rRNA gene sequence analysis indicated that strain AMV4 was a member of the order and was closely related to with a sequence similarity of 97.13 % (pairwise alignment). Phylogenetic analyses showed that strain AMV4 clustered with and was distantly related to the other genera of the family . DNA–DNA hybridization between strains AMV4 and IV-75 showed a relatedness of 28 %. The predominant cellular fatty acids were iso-C (6.9 %), anteiso-C (25.3 %), C (12.9 %), anteiso-C (5.6 %), Cω9 (19.8 %) and Cω6,9,12 (9.1 %). The diagnostic diamino acid in the cell-wall peptidoglycan of strain AMV4 was -diaminopimelic acid. Strain AMV4 contained MK-10(H) as the predominant respiratory quinone. The polar lipids consisted of phosphatidylglycerol, one unidentified glycolipid, two unidentified phospholipids and five unidentified lipids. The DNA G+C content of strain AMV4 was 74.3 mol%. Based on data from this taxonomic study using a polyphasic approach, it is proposed that strain AMV4 represents a novel species of the genus , with the suggested name sp. nov. The type strain is AMV4 ( = CCUG 58430 = DSM 22863 = NBRC 107137).

  • Published Online:
© 2015 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000269
2015-08-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/8/2391.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000269&mimeType=html&fmt=ahah

References

  1. Altschul S.F., Gish W., Miller W., Myers E.W., Lipman D.J. ( 1990;). Basic local alignment search tool. J Mol Biol 215 403–410 [View Article] [PubMed].
     [Google Scholar]
  2. Bligh E.G., Dyer W.J. ( 1959;). A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37 911–917 [View Article] [PubMed].
     [Google Scholar]
  3. Collins M.D., Pirouz T., Goodfellow M., Minnikin D.E. ( 1977;). Distribution of menaquinones in actinomycetes corynebacteria . J Gen Microbiol 100 221–230 [View Article] [PubMed].
     [Google Scholar]
  4. Groth I., Schumann P., Rainey F.A., Martin K., Schuetze B., Augsten K. ( 1997;). Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. Int J Syst Bacteriol 47 1129–1133 [View Article] [PubMed].
     [Google Scholar]
  5. Hasegawa T., Takizawa M., Tanida S. ( 1983;). A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29 319–322 [View Article].
     [Google Scholar]
  6. Kim O.S., Cho Y.J., Lee K., Yoon S.H., Kim M., Na H., Park S.C., Jeon Y.S., Lee J.H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716–721 [View Article] [PubMed].
     [Google Scholar]
  7. Komagata K., Suzuki K. ( 1987;). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19 161–207 [View Article].
     [Google Scholar]
  8. Lane D.J. ( 1991;). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;.
     [Google Scholar]
  9. Lányí B. ( 1987;). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19 1–67. [CrossRef]
    [Google Scholar]
  10. Marmur J. ( 1961;). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3 208–218 [View Article].
     [Google Scholar]
  11. Shivaji S., Ray M.K., Shyamala Rao N., Saisree L., Jagannadham M.V., Seshu Kumar G., Reddy G.S.N., Bhargava P.M. ( 1992;). Sphingobacterium antarcticus sp. nov.: a psychrotrophic bacterium from the soils of Schirmacher Oasis, Antarctica. Int J Syst Bacteriol 42 102–106 [View Article].
     [Google Scholar]
  12. Sly L.I., Blackall L.L., Kraat P.C., Tian-Shen T., Sangkhobol V. ( 1986;). The use of second derivative plots for the determination of mol% guanine plus cytosine of DNA by the thermal denaturation method. J Microbiol Methods 5 139–156 [View Article].
     [Google Scholar]
  13. Smibert R.M., Krieg N.R. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;.
     [Google Scholar]
  14. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28 2731–2739 [View Article] [PubMed].
     [Google Scholar]
  15. Tóth E.M., Kéki Z., Bohus V., Borsodi A.K., Márialigeti K., Schumann P. ( 2012;). Aquipuribacter hungaricus gen. nov., sp. nov., an actinobacterium isolated from the ultrapure water system of a power plant. Int J Syst Evol Microbiol 62 556–562 [View Article] [PubMed].
     [Google Scholar]
  16. Tourova T.P., Antonov A.S. ( 1988;). Identification of microorganisms by rapid DNA–DNA hybridization. Methods Microbiol 19 333–355 [View Article].
     [Google Scholar]
  17. Vandamme P., Pot B., Gillis M., de Vos P., Kersters K., Swings J. ( 1996;). Polyphasic taxonomy, a consensus approach to bacterial systematics. Microbiol Rev 60 407–438 [PubMed].
     [Google Scholar]
  18. Wayne L.G., Brenner D.J., Colwell R.R., Grimont P.A.D., Kandler O., Krichevsky M.I., Moore L.H., Moore W.E.C., Murray R.G.E., other authors. ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37 463–464 [View Article].
     [Google Scholar]
  19. ZoBell C.E. ( 1941;). Studies on marine bacteria. I. The cultural requirements of heterotrophic aerobes. J Mar Res 4 42–75.
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000269
Loading
Aquipuribacter nitratireducens sp. nov., isolated from a soil sample of a mud volcano
Int J Syst Evol Microbiol 65, 2391 (2015); https://doi.org/10.1099/ijs.0.000269
/content/journal/ijsem/10.1099/ijs.0.000269
/content/journal/ijsem/10.1099/ijs.0.000269
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error