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Volume 65, Issue Pt_8

Molecular systematics of marine gregarine apicomplexans from Pacific tunicates, with descriptions of five novel species of Free

Abstract

The eugregarines are a group of apicomplexan parasites that mostly infect the intestines of invertebrates. The high level of morphological variation found within and among species of eugregarines makes it difficult to find consistent and reliable traits that unite even closely related lineages. Based mostly on traits observed with light microscopy, the majority of described eugregarines from marine invertebrates has been classified into a single group, the Lecudinidae. Our understanding of the overall diversity and phylogenetic relationships of lecudinids is very poor, mainly because only a modest amount of exploratory research has been done on the group and very few species of lecudinids have been characterized at the molecular phylogenetic level. In an attempt to understand the diversity of marine gregarines better, we surveyed lecudinids that infect the intestines of Pacific ascidians (i.e. sea squirts) using ultrastructural and molecular phylogenetic approaches; currently, these species fall within one genus, . We collected lecudinid gregarines from six ascidian host species, and our data demonstrated that each host was infected by a different species of : (i) sp. nov., isolated from , (ii) sp. nov., isolated from , (iii) sp. nov., isolated from , (iv) sp. nov., isolated from , (v) cf. , isolated from , and (vi) sp. nov., isolated from . Visualization of the trophozoites with scanning electron microscopy showed that four of these species were covered with epicytic folds, whereas two of the species were covered with a dense pattern of epicytic knobs. The molecular phylogenetic data suggested that species of with surface knobs form a clade that is nested within a paraphyletic assemblage species of with epicytic folds.

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2015-08-01
2024-04-23
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Molecular systematics of marine gregarine apicomplexans from Pacific tunicates, with descriptions of five novel species of Lankesteria
Int J Syst Evol Microbiol 65, 2598 (2015); https://doi.org/10.1099/ijs.0.000300
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