%0 Journal Article %A Yang, Guiqin %A Chen, Junhua %A Zhou, Shungui %T Novibacillus thermophilus gen. nov., sp. nov., a Gram-staining-negative and moderately thermophilic member of the family Thermoactinomycetaceae %D 2015 %J International Journal of Systematic and Evolutionary Microbiology, %V 65 %N Pt_8 %P 2591-2597 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.000306 %I Microbiology Society, %X Two Gram-staining-negative, facultatively anaerobic bacterial strains, SG-1T and SG-2, were isolated from a saline soil sample and a compost sample, respectively. The cells were non-motile rods that occurred singly or in chains, and endospores were not observed under tested growth conditions. Optimum growth occurred at 50 °C, pH 7.5–8.0 and with 5–7 % (w/v) NaCl. The DNA G+C content was 49.5–50.5 mol%. The strains contained MK-7 as the predominant menaquinone and iso-C15 : 0 and anteiso-C15 : 0 as the major fatty acids. The polar lipids consisted mainly of diphosphatidylglycerol and phosphatidylglycerol. The cell-wall peptidoglycan type was A1γ (meso-DAP direct). Phylogenetic analyses revealed that the new isolates belonged to the family Thermoactinomycetaceae, exhibiting low 16S rRNA gene sequence similarity (90.8–91.3 %) to the nearest type strain, Mechercharimyces asporophorigenens YM11-542T, and formed a well-supported lineage that was clearly distinguished from all currently described genera in this family. Based on our polyphasic taxonomic characterization, we propose that strains SG-1T and SG-2 represent a novel genus and species within the family Thermoactinomycetaceae, for which we propose the name Novibacillus thermophilus gen. nov., sp. nov. The type strain of Novibacillus thermophilus is SG-1T ( = KCTC 33118T = CGMCC 1.12771T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.000306