1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 65, Issue Pt_9

sp. nov., isolated from coastal sediment samples Free

Abstract

A novel aerobic, Gram-staining-negative bacterium, designated strain LAM-WHM-ZC, was isolated from coastal sediment samples from the Bohai Sea, near Yantai, China. Cells of LAM-WHM-ZC were non-motile, short-rod- or coccoid-shaped. The temperature and pH ranges for growth were 4–40  °C (optimum: 20–33  °C) and pH 5–9 (optimum: pH 7.5). The strain did not require NaCl for growth but tolerated up to 10 % NaCl (w/v). The major fatty acids of strain LAM-WHM-ZC were summed feature 3, C, C, summed feature 2 and summed feature 8. The predominant respiratory quinone was ubiquinone Q-8. The main polar lipids were diphosphatidyglycerol, phosphatigylethanolamine, phosphatidyglycerol, one phospholipid and four unidentified glycolipids. The DNA G+C content was 59.3 mol% as determined by the melting temperature ( ) method. Analysis of the 16S rRNA gene sequence indicated that the isolate represented a member of the genus and was closely related to KCTC 23013, DSM 15406, KACC 15117 and KCTC 12522 with 97.7 %, 97.1 %, 96.6 % and 96.6 % sequence similarity, respectively. The DNA–DNA hybridization values between strain LAM-WHM-ZC and the four reference strains were 47.4 ± 2.8 %, 33.5 ± 2.2 %, 28.4 ± 1.8 % and 13.7 ± 0.8 %, respectively. Based on its phenotypic and genotypic properties, strain LAM-WHM-ZC is suggested to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is LAM-WHM-ZC ( = ACCC 06516 = JCM 30466).

  • Published Online:
© 2015 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000331
2015-09-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/9/2797.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000331&mimeType=html&fmt=ahah

References

  1. Choi W.C., Kang S.J., Jung Y.T., Oh T.K., Yoon J.H. ( 2011;). Oceanisphaera ostreae sp. nov., isolated from seawater of an oyster farm, and emended description of the genus Oceanisphaera Romanenko et al. 2003. Int J Syst Evol Microbiol 61 2880–2884 [View Article] [PubMed].
     [Google Scholar]
  2. De Ley J., Cattoir H., Reynaerts A. ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12 133–142 [View Article] [PubMed].
     [Google Scholar]
  3. Fang M.X., Zhang W.W., Zhang Y.Z., Tan H.Q., Zhang X.Q., Wu M., Zhu X.F. ( 2012;). Brassicibacter mesophilus gen. nov., sp. nov., a strictly anaerobic bacterium isolated from food industry wastewater. Int J Syst Evol Microbiol 62 3018–3023 [View Article] [PubMed].
     [Google Scholar]
  4. Felsenstein J. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39 783–791 [View Article].
     [Google Scholar]
  5. Fitch W.M. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20 406–416 [View Article].
     [Google Scholar]
  6. Huss V.A.R., Festl H., Schleifer K.H. ( 1983;). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4 184–192 [View Article] [PubMed].
     [Google Scholar]
  7. Kates M. ( 1986). Techniques of Lipidology , 2nd edn.. Amsterdam: Elsevier;.
     [Google Scholar]
  8. Kim O.S., Cho Y.J., Lee K., Yoon S.H., Kim M., Na H., Park S.C., Jeon Y.S., Lee J.H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716–721 [View Article] [PubMed].
     [Google Scholar]
  9. Komagata K., Suzuki K. ( 1987;). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19 161–207. [CrossRef]
    [Google Scholar]
  10. Marmur J. ( 1961;). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3 208–218 [View Article].
     [Google Scholar]
  11. Marmur J., Doty P. ( 1962;). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5 109–118 [View Article] [PubMed].
     [Google Scholar]
  12. Minnikin D.E., O'Donnell A.G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J.H. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2 233–241 [View Article].
     [Google Scholar]
  13. Park S.J., Kang C.H., Nam Y.D., Bae J.W., Park Y.H., Quan Z.X., Moon D.S., Kim H.J., Roh D.H., Rhee S.K. ( 2006;). Oceanisphaera donghaensis sp. nov., a halophilic bacterium from the East Sea, Korea. Int J Syst Evol Microbiol 56 895–898 [View Article] [PubMed].
     [Google Scholar]
  14. Romanenko L.A., Schumann P., Zhukova N.V., Rohde M., Mikhailov V.V., Stackebrandt E. ( 2003;). Oceanisphaera litoralis gen. nov., sp. nov., a novel halophilic bacterium from marine bottom sediments. Int J Syst Evol Microbiol 53 1885–1888 [View Article] [PubMed].
     [Google Scholar]
  15. Ruan Z., Wang Y., Song J., Jiang S., Wang H., Li Y., Zhao B., Jiang R., Zhao B. ( 2014;). Kurthia huakuii sp. nov., isolated from biogas slurry, and emended description of the genus Kurthia . Int J Syst Evol Microbiol 64 518–521 [View Article] [PubMed].
     [Google Scholar]
  16. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406–425 [PubMed].
     [Google Scholar]
  17. Sakamoto M., Suzuki M., Umeda M., Ishikawa I., Benno Y. ( 2002;). Reclassification of Bacteroides forsythus (Tanner et al. 1986) as Tannerella forsythensis corrig., gen. nov., comb. nov. Int J Syst Evol Microbiol 52 841–849 [View Article] [PubMed].
     [Google Scholar]
  18. Shin N.R., Whon T.W., Roh S.W., Kim M.S., Kim Y.O., Bae J.W. ( 2012;). Oceanisphaera sediminis sp. nov., isolated from marine sediment. Int J Syst Evol Microbiol 62 1552–1557 [View Article] [PubMed].
     [Google Scholar]
  19. Smibert R.M., Krieg N.R. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;.
     [Google Scholar]
  20. Srinivas T.N.R., Reddy P.V., Begum Z., Manasa P., Shivaji S. ( 2012;). Oceanisphaera arctica sp. nov., isolated from Arctic marine sediment, and emended description of the genus Oceanisphaera . Int J Syst Evol Microbiol 62 1926–1931 [View Article] [PubMed].
     [Google Scholar]
  21. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30 2725–2729 [View Article] [PubMed].
     [Google Scholar]
  22. Thompson J.D., Higgins D.G., Gibson T.J. ( 1994;). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22 4673–4680 [View Article] [PubMed].
     [Google Scholar]
  23. Tittsler R.P., Sandholzer L.A. ( 1936;). The use of semi-solid agar for the detection of bacterial motility. J Bacteriol 31 575–580 [PubMed].
     [Google Scholar]
  24. Weisburg W.G., Barns S.M., Pelletier D.A., Lane D.J. ( 1991;). 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173 697–703 [PubMed].
     [Google Scholar]
  25. Xu X.W., Huo Y.Y., Wang C.S., Oren A., Cui H.L., Vedler E., Wu M. ( 2011;). Pelagibacterium halotolerans gen. nov., sp. nov. and Pelagibacterium luteolum sp. nov., novel members of the family Hyphomicrobiaceae . Int J Syst Evol Microbiol 61 1817–1822 [View Article] [PubMed].
     [Google Scholar]
  26. Xu Z., Zhang X.Y., Su H.N., Yu Z.C., Liu C., Li H., Chen X.L., Song X.Y., Xie B.B., other authors. ( 2014;). Oceanisphaera profunda sp. nov., a marine bacterium isolated from deep-sea sediment, and emended description of the genus Oceanisphaera . Int J Syst Evol Microbiol 64 1252–1256 [View Article] [PubMed].
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000331
Loading
Oceanisphaera psychrotolerans sp. nov., isolated from coastal sediment samples
Int J Syst Evol Microbiol 65, 2797 (2015); https://doi.org/10.1099/ijs.0.000331
/content/journal/ijsem/10.1099/ijs.0.000331
/content/journal/ijsem/10.1099/ijs.0.000331
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error