RT Journal Article SR Electronic(1) A1 Zhou, Shan A1 Wang, Huimin A1 Wang, Yanwei A1 Ma, Kedong A1 He, Mingxiong A1 Chen, Xiaorong A1 Kong, Delong A1 Guo, Xiang A1 Ruan, Zhiyong A1 Zhao, BinYR 2015 T1 Oceanisphaera psychrotolerans sp. nov., isolated from coastal sediment samples JF International Journal of Systematic and Evolutionary Microbiology, VO 65 IS Pt_9 SP 2797 OP 2802 DO https://doi.org/10.1099/ijs.0.000331 PB Microbiology Society, SN 1466-5034, AB A novel aerobic, Gram-staining-negative bacterium, designated strain LAM-WHM-ZCT, was isolated from coastal sediment samples from the Bohai Sea, near Yantai, China. Cells of LAM-WHM-ZCT were non-motile, short-rod- or coccoid-shaped. The temperature and pH ranges for growth were 4–40  °C (optimum: 20–33  °C) and pH 5–9 (optimum: pH 7.5). The strain did not require NaCl for growth but tolerated up to 10 % NaCl (w/v). The major fatty acids of strain LAM-WHM-ZCT were summed feature 3, C12 : 0, C16 : 0, summed feature 2 and summed feature 8. The predominant respiratory quinone was ubiquinone Q-8. The main polar lipids were diphosphatidyglycerol, phosphatigylethanolamine, phosphatidyglycerol, one phospholipid and four unidentified glycolipids. The DNA G+C content was 59.3 mol% as determined by the melting temperature (T m) method. Analysis of the 16S rRNA gene sequence indicated that the isolate represented a member of the genus Oceanisphaera and was closely related to Oceanisphaera arctica KCTC 23013T, Oceanisphaera litoralis DSM 15406T, Oceanisphaera sediminis KACC 15117T and Oceanisphaera donghaensis KCTC 12522T with 97.7 %, 97.1 %, 96.6 % and 96.6 % sequence similarity, respectively. The DNA–DNA hybridization values between strain LAM-WHM-ZCT and the four reference strains were 47.4 ± 2.8 %, 33.5 ± 2.2 %, 28.4 ± 1.8 % and 13.7 ± 0.8 %, respectively. Based on its phenotypic and genotypic properties, strain LAM-WHM-ZCT is suggested to represent a novel species of the genus Oceanisphaera, for which the name Oceanisphaera psychrotolerans sp. nov. is proposed. The type strain is LAM-WHM-ZCT ( = ACCC 06516T = JCM 30466T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.000331