1887

Abstract

A novel bacterial strain, designated EC29, was isolated from the brown alga collected on Jeju Island, Republic of Korea. Cells of strain EC29 were Gram-stain-negative, aerobic, rod-shaped and motile by gliding. Growth was observed at 10–30 °C (optimum, 20–25 °C), at pH 6.0–9.5 (optimum, pH 7.5) and in the presence of 1–5 % (w/v) NaCl. Phylogenetic analyses based on the 16S rRNA gene sequence revealed that the strain belonged to the genus . Strain EC29 exhibited the highest 16S rRNA gene sequence similarities, of 96.5–97.8 %, to the type strains of EM106, KMM 6211 and KMM 6390. Strain EC29 exhibited < 27 % DNA–DNA relatedness with EM106 and KMM 6211. The predominant fatty acids of strain EC29 were iso-C, iso-C G, C, iso-C 3-OH, iso-C 3-OH and anteiso-C. The DNA G+C content was 31.1 mol% and the major respiratory quinone was menaquinone-6 (MK-6). Based on a polyphasic study, strain EC29 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is EC29 ( = KCTC 32172 = JCM 18703).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000334
2015-09-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/9/2791.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000334&mimeType=html&fmt=ahah

References

  1. Begum Z., Srinivas T.N.R., Manasa P., Sailaja B., Sunil B., Prasad S., Shivaji S. ( 2013;). Winogradskyella psychrotolerans sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from Arctic sediment. Int J Syst Evol Microbiol 63 16461652 [View Article] [PubMed] .
    [Google Scholar]
  2. Bernardet J.-F. ( 2011;). Family I. Flavobacteriaceae Reichenbach 1992. . In Bergey's Manual of Systematic Bacteriology vol. 4 , 2nd edn.. pp. 106111. Edited by Krieg N. R., Ludwig W., Whitman W. B., Hedlund B. P., Paster B. J., Staley J. T., Ward N., Brown D., Parte A. New York: Springer;.
    [Google Scholar]
  3. Bernardet J.-F., Nakagawa Y. ( 2006;). An introduction to the family Flavobacteriaceae . . In The Prokaryotes. A Handbook on the Biology of Bacteria vol. 7 , 3rd edn.. pp. 455480. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. New York: Springer;.
    [Google Scholar]
  4. Bernardet J.-F., Nakagawa Y., Holmes B., Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes. ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52 10491070 [View Article] [PubMed] .
    [Google Scholar]
  5. Bowman J.P. ( 2000;). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50 18611868 [PubMed]. [CrossRef]
    [Google Scholar]
  6. Christensen H., Angen O., Mutters R., Olsen J.E., Bisgaard M. ( 2000;). DNA-DNA hybridization determined in micro-wells using covalent attachment of DNA. Int J Syst Evol Microbiol 50 10951102 [View Article] [PubMed] .
    [Google Scholar]
  7. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39 224229 [View Article].
    [Google Scholar]
  8. Felsenstein J. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17 368376 [View Article] [PubMed] .
    [Google Scholar]
  9. Felsenstein J. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39 783791 [View Article].
    [Google Scholar]
  10. Hall T.A. ( 1999;). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41 9598.
    [Google Scholar]
  11. Ivanova E.P., Christen R., Gorshkova N.M., Zhukova N.V., Kurilenko V.V., Crawford R.J., Mikhailov V.V. ( 2010;). Winogradskyella exilis sp. nov., isolated from the starfish Stellaster equestris, and emended description of the genus Winogradskyella . Int J Syst Evol Microbiol 60 15771580 [View Article] [PubMed] .
    [Google Scholar]
  12. Kang C.-H., Lee S.-Y., Yoon J.-H. ( 2013;). Winogradskyella litorisediminis sp. nov., isolated from coastal sediment. Int J Syst Evol Microbiol 63 17931799 [View Article] [PubMed] .
    [Google Scholar]
  13. Kim S.B., Nedashkovskaya O.I. ( 2010;). Winogradskyella pacifica sp. nov., a marine bacterium of the family Flavobacteriaceae . Int J Syst Evol Microbiol 60 19481951 [View Article] [PubMed] .
    [Google Scholar]
  14. Kim J.-Y., Oh D.-C. ( 2012;). Winogradskyella jejuensis sp. nov., a marine bacterium isolated from a brown alga Carpopeltis affinis . J Microbiol 50 888892 [View Article] [PubMed] .
    [Google Scholar]
  15. Kim O.S., Cho Y.J., Lee K., Yoon S.H., Kim M., Na H., Park S.C., Jeon Y.S., Lee J.H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716721 [View Article] [PubMed] .
    [Google Scholar]
  16. Kim S.-J., Choi Y.-R., Park S.-J., Kim J.-G., Shin K.-S., Roh D.-H., Rhee S.-K. ( 2013;). Winogradskyella pulchriflava sp. nov., isolated from marine sediment. Int J Syst Evol Microbiol 63 30623068 [View Article] [PubMed] .
    [Google Scholar]
  17. Kluge A.G., Farris F.S. ( 1969;). Quantitative phyletics and the evolution of anurans. Syst Zool 18 132 [View Article].
    [Google Scholar]
  18. Komagata K., Suzuki K. ( 1987;). Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol 19 161207 [View Article].
    [Google Scholar]
  19. Lau S.C.K., Tsoi M.M.Y., Li X., Plakhotnikova I., Dobretsov S., Lau K.W.K., Wu M., Wong P.-K., Pawlik J.R., Qian P.-Y. ( 2005;). Winogradskyella poriferorum sp. nov., a novel member of the family Flavobacteriaceae isolated from a sponge in the Bahamas. Int J Syst Evol Microbiol 55 15891592 [View Article] [PubMed] .
    [Google Scholar]
  20. Lee S.-Y., Park S., Oh T.-K., Yoon J.-H. ( 2012;). Winogradskyella aquimaris sp. nov., isolated from seawater. Int J Syst Evol Microbiol 62 18141818 [View Article] [PubMed] .
    [Google Scholar]
  21. Lee D.-H., Cho S.J., Kim S.M., Lee S.B. ( 2013;). Winogradskyella damuponensis sp. nov., isolated from seawater. Int J Syst Evol Microbiol 63 321326 [View Article] [PubMed] .
    [Google Scholar]
  22. Marmur J. ( 1961;). A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3 208218 [View Article].
    [Google Scholar]
  23. Marmur J., Doty P. ( 1962;). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5 109118 [View Article] [PubMed] .
    [Google Scholar]
  24. Minnikin D.E., O'Donnell A.G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J.H. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2 233241 [View Article].
    [Google Scholar]
  25. Nedashkovskaya O.I., Kim S.B., Han S.K., Snauwaert C., Vancanneyt M., Swings J., Kim K.O., Lysenko A.M., Rohde M., other authors. ( 2005;). Winogradskyella thalassocola gen. nov., sp. nov., Winogradskyella epiphytica sp. nov. and Winogradskyella eximia sp. nov., marine bacteria of the family Flavobacteriaceae . Int J Syst Evol Microbiol 55 4955 [View Article] [PubMed] .
    [Google Scholar]
  26. Nedashkovskaya O.I., Vancanneyt M., Kim S.B., Zhukova N.V. ( 2009;). Winogradskyella echinorum sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from the sea urchin Strongylocentrotus intermedius . Int J Syst Evol Microbiol 59 14651468 [View Article] [PubMed] .
    [Google Scholar]
  27. Nedashkovskaya O.I., Kukhlevskiy A.D., Zhukova N.V. ( 2012;). Winogradskyella ulvae sp. nov., an epiphyte of a Pacific seaweed, and emended descriptions of the genus Winogradskyella and Winogradskyella thalassocola, Winogradskyella echinorum, Winogradskyella exilis and Winogradskyella eximia . Int J Syst Evol Microbiol 62 14501456 [View Article] [PubMed] .
    [Google Scholar]
  28. Park S., Yoon J.-H. ( 2013;). Winogradskyella undariae sp. nov., a member of the family Flavobacteriaceae isolated from a brown algae reservoir. Antonie van Leeuwenhoek 104 619626 [View Article] [PubMed] .
    [Google Scholar]
  29. Park S., Park J.-M., Won S.-M., Bae K.S., Yoon J.-H. ( 2014;). Winogradskyella wandonensis sp. nov., isolated from a tidal flat. Int J Syst Evol Microbiol 64 15201525 [View Article] [PubMed] .
    [Google Scholar]
  30. Pinhassi J., Nedashkovskaya O.I., Hagström A., Vancanneyt M. ( 2009;). Winogradskyella rapida sp. nov., isolated from protein-enriched seawater. Int J Syst Evol Microbiol 59 21802184 [View Article] [PubMed] .
    [Google Scholar]
  31. Romanenko L.A., Tanaka N., Frolova G.M., Mikhailov V.V. ( 2009;). Winogradskyella arenosi sp. nov., a member of the family Flavobacteriaceae isolated from marine sediments from the Sea of Japan. Int J Syst Evol Microbiol 59 14431446 [View Article] [PubMed] .
    [Google Scholar]
  32. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406425 [PubMed].
    [Google Scholar]
  33. Sasser M. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . In MIDI Inc., Newark, DE:.
    [Google Scholar]
  34. Smibert R.M., Krieg N.R. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P. Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  35. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30 27252729 [View Article] [PubMed] .
    [Google Scholar]
  36. Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., Higgins D.G. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25 48764882 [View Article] [PubMed] .
    [Google Scholar]
  37. Wayne L.G., Brenner D.J., Colwell R.R., Grimont P.A.D., Kandler O., Krichevsky M.I., Moore L.H., Moore W.E.C., Murray R.G.E., other authors. ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37 463464 [View Article].
    [Google Scholar]
  38. Weisburg W.G., Barns S.M., Pelletier D.A., Lane D.J. ( 1991;). 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173 697703 [PubMed].
    [Google Scholar]
  39. Yoon J.-H., Lee S.-Y. ( 2012;). Winogradskyella multivorans sp. nov., a polysaccharide-degrading bacterium isolated from seawater of an oyster farm. Antonie van Leeuwenhoek 102 231238 [View Article] [PubMed] .
    [Google Scholar]
  40. Yoon B.-J., Byun H.-D., Kim J.-Y., Lee D.-H., Kahng H.-Y., Oh D.-C. ( 2011;). Winogradskyella lutea sp. nov., isolated from seawater, and emended description of the genus Winogradskyella . Int J Syst Evol Microbiol 61 15391543 [View Article] [PubMed] .
    [Google Scholar]
  41. ZoBell C.E. ( 1941;). Studies on marine bacteria. I. The cultural requirements of heterotrophic aerobes. J Mar Res 4 4275.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000334
Loading
/content/journal/ijsem/10.1099/ijs.0.000334
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error