1887

Abstract

Two strictly anaerobic bacterial strains, designated SW512 and W72, were isolated from a laboratory-scale H-producing up-flow anaerobic sludge blanket (UASB) reactor. Cells were Gram-stain-negative, non-motile and 0.3–0.4×2.0–14.5 μm; they did not form spores. Both strains grew at 24–45 °C (no growth at ≤22 °C or ≥46 °C), with optimum growth at 37 °C. The pH range for growth was 4.5–9.0 (no growth at pH≤4.2 or pH≥9.3), with optimum growth at pH 6.5–7.0. Several kinds of mono-, di- and oligosaccharides supported growth. The main end products of glucose fermentation were ethanol, acetate, hydrogen and carbon dioxide (according to the equation 1 mol glucose→1.1 mol acetate+0.6 mol ethanol+2.6 mol H+1.6 mol CO). The DNA G+C contents of strains SW512 and W72 were 41.9±0.5 and 42.8±0.4 mol% ( method), respectively. Phylogenetic analysis based on 16S rRNA gene sequence similarities indicated that the two strains represent a new phyletic sublineage within the family ‘’, with <91.3 % 16S rRNA gene sequence similarity to recognized species. On the basis of the polyphasic evidence in this study, it is proposed that the two strains represent a novel species in a new genus, for which the name gen. nov., sp. nov. is proposed; the type strain of the type species is SW512 (=AS 1.5070=JCM 14861).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000349-0
2009-02-01
2024-03-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/2/295.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000349-0&mimeType=html&fmt=ahah

References

  1. Chen, S. & Dong, X.(2004).Acetanaerobacterium elongatum gen. nov., sp. nov., from paper mill waste water. Int J Syst Evol Microbiol 54, 2257–2262.[CrossRef] [Google Scholar]
  2. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  3. Felsenstein, J.(1993).phylip (phylogenetic inference package), version 3.5c. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  4. Hungate, R. E.(1969). A roll tube method for cultivation of strict anaerobes. Methods Microbiol 3B, 117–132. [Google Scholar]
  5. Johnson, M. J., Thatcher, E. & Cox, M. E.(1995). Techniques for controlling variability in gram staining of obligate anaerobes. J Clin Microbiol 33, 755–758. [Google Scholar]
  6. Kämpfer, P., Steiof, M. & Dott, W.(1991). Microbiological characterization of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. Microb Ecol 21, 227–251.[CrossRef] [Google Scholar]
  7. Kaneda, T.(1991). Iso- and anteiso-fatty acids in bacteria: biosynthesis, function, and taxonomic significance. Microbiol Rev 55, 288–302. [Google Scholar]
  8. Kumar, S., Tamura, K., Jakobsen, I. B. & Nei, M.(2001).mega2: molecular evolutionary genetics analysis software. Bioinformatics 17, 1244–1245.[CrossRef] [Google Scholar]
  9. Lawson, P. A., Song, Y., Liu, C., Molitoris, D. R., Vaisanen, M. L., Collins, M. D. & Finegold, S. M.(2004).Anaerotruncus colihominis gen. nov., sp. nov., from human faeces. Int J Syst Evol Microbiol 54, 413–417.[CrossRef] [Google Scholar]
  10. Marmur, J.(1961). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef] [Google Scholar]
  11. Marmur, J. & Doty, P.(1962). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef] [Google Scholar]
  12. McInerney, M. J., Bryant, M. P. & Pfennig, N.(1979). Anaerobic bacterium that degrades fatty acids in syntrophic association with methanogens. Arch Microbiol 122, 129–135.[CrossRef] [Google Scholar]
  13. Miller, L. T.(1982). Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxyl acids. J Clin Microbiol 16, 584–586. [Google Scholar]
  14. Sasser, M.(1990).Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI.
  15. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000349-0
Loading
/content/journal/ijsem/10.1099/ijs.0.000349-0
Loading

Data & Media loading...

Supplements

vol. , part 2, pp. 295 - 299

Phylogenetic dendrogram of sp. nov. SW512 and related species based on 16S rRNA gene similarity. [ PDF] 37 KB



PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error