@article{mbs:/content/journal/ijsem/10.1099/ijs.0.000349-0, author = "Song, Lei and Dong, Xiuzhu", title = "Hydrogenoanaerobacterium saccharovorans gen. nov., sp. nov., isolated from H2-producing UASB granules", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2009", volume = "59", number = "2", pages = "295-299", doi = "https://doi.org/10.1099/ijs.0.000349-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.000349-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "UASB, up-flow anaerobic sludge blanket", abstract = "Two strictly anaerobic bacterial strains, designated SW512T and W72, were isolated from a laboratory-scale H2-producing up-flow anaerobic sludge blanket (UASB) reactor. Cells were Gram-stain-negative, non-motile and 0.3–0.4×2.0–14.5 μm; they did not form spores. Both strains grew at 24–45 °C (no growth at ≤22 °C or ≥46 °C), with optimum growth at 37 °C. The pH range for growth was 4.5–9.0 (no growth at pH≤4.2 or pH≥9.3), with optimum growth at pH 6.5–7.0. Several kinds of mono-, di- and oligosaccharides supported growth. The main end products of glucose fermentation were ethanol, acetate, hydrogen and carbon dioxide (according to the equation 1 mol glucose→1.1 mol acetate+0.6 mol ethanol+2.6 mol H2+1.6 mol CO2). The DNA G+C contents of strains SW512T and W72 were 41.9±0.5 and 42.8±0.4 mol% (T m method), respectively. Phylogenetic analysis based on 16S rRNA gene sequence similarities indicated that the two strains represent a new phyletic sublineage within the family ‘Ruminococcaceae’, with <91.3 % 16S rRNA gene sequence similarity to recognized species. On the basis of the polyphasic evidence in this study, it is proposed that the two strains represent a novel species in a new genus, for which the name Hydrogenoanaerobacterium saccharovorans gen. nov., sp. nov. is proposed; the type strain of the type species is SW512T (=AS 1.5070T=JCM 14861T).", }