1887

Abstract

Rhizobial strains isolated from effective root nodules of field-grown lentil () from different parts of Bangladesh were previously analysed using sequences of the 16S rRNA gene, three housekeeping genes (, and ) and three nodulation genes (, and ), DNA fingerprinting and phenotypic characterization. Analysis of housekeeping gene sequences and DNA fingerprints indicated that the strains belonged to three novel clades in the genus . In present study, a representative strain from each clade was further characterized by determination of cellular fatty acid compositions, carbon substrate utilization patterns and DNA–DNA hybridization and average nucleotide identity (ANI) analyses from whole-genome sequences. DNA–DNA hybridization showed 50–62 % relatedness to their closest relatives (the type strains of and ) and 50–60 % relatedness to each other. These results were further supported by ANI values, based on genome sequencing, which were 87–92 % with their close relatives and 88–89 % with each other. On the basis of these results, three novel species, sp. nov. (type strain BLR27 = LMG 28441 = DSM 29286), sp. nov. (type strain BLR175 = LMG 28442 = DSM 29287) and sp. nov. (type strain BLR195 = LMG 28443 = DSM 29288), are proposed. These species share common nodulation genes (, and ) that are similar to those of the symbiovar .

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2015-09-01
2024-03-28
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