RT Journal Article SR Electronic(1) A1 Zhao, Junwei A1 Guo, Lifeng A1 Liu, Chongxi A1 Bai, Lu A1 Han, Chuanyu A1 Li, Jiansong A1 Xiang, Wensheng A1 Wang, XiangjingYR 2015 T1 Streptomyces tyrosinilyticus sp. nov., a novel actinomycete isolated from river sediment JF International Journal of Systematic and Evolutionary Microbiology, VO 65 IS Pt_9 SP 3091 OP 3096 DO https://doi.org/10.1099/ijs.0.000385 PB Microbiology Society, SN 1466-5034, AB A novel actinomycete, designated strain NEAU-Jh3-20T, was isolated from river sediment collected from South river in Jilin Province, north China and characterized using a polyphasic approach. Phylogenetic analysis, based on 16S rRNA gene sequences, indicated that strain NEAU-Jh3-20T should be assigned to the genus Streptomyces and forms a distinct branch with its closest neighbour Streptomyces vitaminophilus DSM 41686T(97.09 %). Moreover, key morphological and chemotaxonomic properties also confirmed the affiliation of strain NEAU-Jh3-20T to the genus Streptomyces. The cell wall contained ll-diaminopimelic acid and the whole-cell hydrolysates were glucose and ribose. The phospholipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides and an unidentified phospholipid. The predominant menaquinones were MK-9(H8) and MK-9(H6). The major fatty acids were C16 : 0, C18 : 0, anteiso-C15 : 0 and anteiso-C17 : 0. The DNA G+C content was 72.2 mol%. A combination of DNA–DNA hybridization results and some phenotypic characteristics demonstrated that strain NEAU-Jh3-20T could be distinguished from its closest phylogenetic relative. Therefore, it is proposed that strain NEAU-Jh3-20T represents a novel species of the genus Streptomyces, for which the name Streptomyces tyrosinilyticus sp. nov. is proposed. The type strain is NEAU-Jh3-20T ( = CGMCC 4.7201T = DSM 42170T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.000385