RT Journal Article SR Electronic(1) A1 Wiese, Jutta A1 Thiel, Vera A1 Gärtner, Andrea A1 Schmaljohann, Rolf A1 Imhoff, Johannes F.YR 2009 T1 Kiloniella laminariae gen. nov., sp. nov., an alphaproteobacterium from the marine macroalga Laminaria saccharina JF International Journal of Systematic and Evolutionary Microbiology, VO 59 IS 2 SP 350 OP 356 DO https://doi.org/10.1099/ijs.0.001651-0 PB Microbiology Society, SN 1466-5034, AB A novel alphaproteobacterium, strain LD81T, was isolated from the marine macroalga Laminaria saccharina. The bacterium is mesophilic and shows a typical marine growth response. It is a chemoheterotrophic aerobe with the potential for denitrification. Growth optima are 25 °C, pH 5.5 and 3 % NaCl. Strain LD81T has a unique phylogenetic position, not fitting any of the known families of the Alphaproteobacteria. The 16S rRNA gene sequence revealed a distant relationship to species of several orders of the Alphaproteobacteria, with less than 90 % sequence similarity. Phylogenetically, strain LD81T is related to the type strains of Terasakiella pusilla (88.4 % 16S rRNA gene sequence similarity) and the three Thalassospira species (88.9–89.2 %). It forms a cluster with these bacteria and a novel as-yet undescribed isolate (KOPRI 13522; 96.6 % sequence similarity). Strain LD81T has a relatively low DNA G+C content (51.1 mol%) and, due to its distant phylogenetic position from all other alphaproteobacteria, strain LD81T (=NCIMB 14374T =JCM 14845T) is considered as the type strain of a novel species within a new genus, for which the name Kiloniella laminariae gen. nov., sp. nov. is proposed. The genus Kiloniella represents the type of the new family Kiloniellaceae fam. nov. and order Kiloniellales ord. nov., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.001651-0