@article{mbs:/content/journal/ijsem/10.1099/ijs.0.001677-0, author = "Mochimaru, Hanako and Tamaki, Hideyuki and Hanada, Satoshi and Imachi, Hiroyuki and Nakamura, Kohei and Sakata, Susumu and Kamagata, Yoichi", title = "Methanolobus profundi sp. nov., a methylotrophic methanogen isolated from deep subsurface sediments in a natural gas field", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2009", volume = "59", number = "4", pages = "714-718", doi = "https://doi.org/10.1099/ijs.0.001677-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.001677-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A mesophilic, methylotrophic methanogen, strain MobMT, was isolated from a natural gas field in Japan. Strain MobMT grew on methanol and methylamines, but not on H2/CO2, formate, acetate or dimethyl sulfide. The cells were motile, irregular cocci (diameter, 0.9–1.2 μm) and occurred singly, in pairs, as tetracocci or (occasionally) as aggregates. Strain MobMT grew at 9–37 °C (optimally at 30 °C) and at pH 6.1–7.8 (optimally at pH 6.5). Sodium and magnesium were required for growth, at 0.1–1.0 M Na+ (optimally at 0.35 M) and 10–400 mM Mg2+ (optimally at 15–25 mM). The G+C content of the genomic DNA was 42.4 mol%. 16S rRNA gene sequencing revealed that the isolate is a member of the genus Methanolobus, but distinct from its closest neighbours, Methanolobus tindarius DSM 2278T (sequence similarity, 98.0 %) and Methanolobus vulcani DSM 3029T (98.1 %). On the basis of phenotypic and phylogenetic features of MobMT, it is clear that this strain represents a novel species of the genus Methanolobus, for which the name Methanolobus profundi sp. nov. is proposed. The type strain is MobMT (=DSM 21213T=NBRC 104158T).", }