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Volume 59, Issue 7

sp. nov., a moderately halophilic bacterium isolated from a sea urchin Free

Abstract

A Gram-positive-staining, moderately halophilic, strictly aerobic, catalase- and oxidase-positive, rod-shaped bacterium, designated strain JSM 076056, was isolated from a sea urchin collected from the South China Sea. Cells were motile by means of peritrichous flagella and formed ellipsoidal endospores lying in subterminal swollen sporangia. Strain JSM 076056 was able to grow at salinities of 2–25 % (w/v) total salts and at pH 6.0–10.0 and 15–40 °C; optimum growth was observed with 5–10 % (w/v) total salts and at pH 7.0–8.0 and 25–30 °C. It was incapable of growing with NaCl as the sole salt. -Diaminopimelic acid was present in the cell-wall peptidoglycan. The major cellular fatty acids were iso-C, iso-C, iso-C anteiso-C and C 7 alcohol. The predominant respiratory quinone was MK-7 and the genomic DNA G+C content was 45.5 mol%. A phylogenetic analysis based on 16S rRNA gene sequences indicated that strain JSM 076056 belonged to the family and was related most closely to the type strains of the two recognized species of the genus , namely BH030062 (96.4 % sequence similarity) and BH030004 (96.2 %); these three strains formed a robust cluster in the phylogenetic tree. In combination, the phylogenetic, phenotypic and chemotaxonomic data indicate that strain JSM 076056 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JSM 076056 (=CCTCC AA 207029 =DSM 19796 =KCTC 13190).

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2009-07-01
2024-04-24
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Pontibacillus halophilus sp. nov., a moderately halophilic bacterium isolated from a sea urchin
Int J Syst Evol Microbiol 59, 1635 (2009); https://doi.org/10.1099/ijs.0.002469-0
/content/journal/ijsem/10.1099/ijs.0.002469-0
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vol. , part 7, pp. 1635 - 1639

Fatty acid compositions of strain JSM 076056 and type strains of the genus . [PDF](32 KB)

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