@article{mbs:/content/journal/ijsem/10.1099/ijs.0.005553-0, author = "Dadhwal, Mandeep and Jit, Simran and Kumari, Hansi and Lal, Rup", title = "Sphingobium chinhatense sp. nov., a hexachlorocyclohexane (HCH)-degrading bacterium isolated from an HCH dumpsite", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2009", volume = "59", number = "12", pages = "3140-3144", doi = "https://doi.org/10.1099/ijs.0.005553-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.005553-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "HCH, hexachlorocyclohexane", keywords = "FAME, fatty acid methyl ester", abstract = "A yellow-pigmented, hexachlorocyclohexane (HCH)-degrading bacterium, strain IP26T, was isolated from an HCH dumpsite and subjected to a polyphasic analysis in order to determine its taxonomic position. Strain IP26T showed maximum 16S rRNA gene sequence similarity with Sphingobium francense Sp+T (98.5 %), Sphingobium japonicum UT26T (98.4 %) and Sphingobium indicum B90AT (98.2 %). Phylogenetic analysis based on 16S rRNA gene sequences also showed that strain IP26T formed a cluster with these three HCH-degrading strains. Chemotaxonomic data (major polyamine, spermidine; major quinone, ubiquinone with ten isoprene units; major polar lipids, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, diphosphatidylglycerol, phosphotidylcholine; and presence of 2-hydroxy fatty acid) supported inclusion of strain IP26T in the genus Sphingobium. However, the results of DNA–DNA hybridization and morphological and biochemical tests clearly allowed phenotypic and genotypic differentiation of strain IP26T from recognized species of the genus Sphingobium. Strain IP26T thus represents a novel species of the genus Sphingobium for which the name Sphingobium chinhatense sp. nov. is proposed. The type strain is IP26T (=MTCC8598T =CCM 7432T).", }