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Volume 59, Issue 8

sp. nov., isolated from homemade compost Free

Abstract

Strain DC-200 was isolated from homemade compost produced from kitchen refuse and characterized using a polyphasic approach. The isolate was a Gram-positive motile short rod, facultatively aerobic, catalase-positive and oxidase-negative, and was able to grow at 10–37 °C, pH 6.0–9.5 and with up to 5 % of NaCl. The peptidoglycan was of the type B1 alpha and the muramic acid residues were glycolylated. The major fatty acids were anteiso-C and anteiso-C. The predominant respiratory menaquinones were MK-11 and MK-12. The G+C content of the genomic DNA was 70 mol%. Based on the analysis of the 16S rRNA gene sequence, the closest phylogenetic neighbours of strain DC-200 were A5E-52 (98.7 %) and KV-492 (98.2 %). The phenetic characterization of the isolate supports its inclusion within the genus ; however, its distinctive phenotypic features and the results from the 16S rRNA gene sequence analysis and the DNA–DNA hybridization study suggest that the isolate represents a novel species. The name sp. nov. is proposed. The type strain is DC-200 (=DSM 19600=LMG 24557).

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Keyword(s): MALDI-TOF, matrix-assisted laser-desorption/ionization time-of-flight
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2009-08-01
2024-04-24
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Microbacterium invictum sp. nov., isolated from homemade compost
Int J Syst Evol Microbiol 59, 2036 (2009); https://doi.org/10.1099/ijs.0.005561-0
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