@article{mbs:/content/journal/ijsem/10.1099/ijs.0.005777-0, author = "Drzyzga, Oliver and Navarro Llorens, J. María and Fernández de las Heras, Laura and García Fernández, Esther and Perera, Julián", title = "Gordonia cholesterolivorans sp. nov., a cholesterol-degrading actinomycete isolated from sewage sludge", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2009", volume = "59", number = "5", pages = "1011-1015", doi = "https://doi.org/10.1099/ijs.0.005777-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.005777-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "The taxonomic position of the cholesterol-degrading strain Chol-3T, isolated from a sewage sludge sample, was clarified using a polyphasic taxonomic approach. Phylogenetic analysis of its 16S rRNA gene sequence, whole-cell fatty acid profile and mycolic acid composition revealed that this isolate is a member of the genus Gordonia with the species Gordonia sihwensis, G. hydrophobica and G. shandongensis being the nearest phylogenetic neighbours. The results of DNA–DNA hybridization against its phylogenetically closest neighbours as well as the results of physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain Chol-3T from the other Gordonia species with validly published names. Strain Chol-3T therefore merits recognition as a member of a novel species within the genus Gordonia, for which the name Gordonia cholesterolivorans sp. nov. is proposed. The type strain is Chol-3T (=CECT 7408T =DSM 45229T).", }