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Volume 59, Issue 5

gen. nov., sp. nov., an extremely thermophilic, facultatively lithoautotrophic, anaerobic bacterium from the Uzon Caldera, Kamchatka Free

Abstract

An extremely thermophilic, strictly anaerobic, facultatively chemolithoautotrophic bacterium designated strain DS was isolated from Treshchinnyi Spring, one of the hottest springs of the Uzon Caldera (Kamchatka, Russia). Cells of the novel organism were Gram-negative rods, about 1.0–1.2 μm long and 0.5 μm wide. The temperature range for growth was 52–82 °C, with an optimum at 75 °C. Growth was observed at pH 6.8–7.4, and the optimum pH was 7.0–7.2. Strain DS was able to grow lithoautotrophically with hydrogen in the presence of CO as a carbon source and thiosulfate or elemental sulfur as an electron acceptor. It also grew well with ethanol, fumarate, succinate or malate in the presence of thiosulfate. Yeast extract was not required for growth and did not stimulate growth. The genomic DNA G+C content was 35.2 mol%. Phylogenetic analysis of the 16S rRNA gene sequence indicated that the novel organism was a member of the family . On the basis of phylogenetic and physiological considerations, it is proposed that strain DS represents a new genus and species, gen. nov., sp. nov. The type strain of is DS (=DSM 19393 =VKM B-2460).

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2009-05-01
2024-04-20
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Caldimicrobium rimae gen. nov., sp. nov., an extremely thermophilic, facultatively lithoautotrophic, anaerobic bacterium from the Uzon Caldera, Kamchatka
Int J Syst Evol Microbiol 59, 1040 (2009); https://doi.org/10.1099/ijs.0.006072-0
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vol. , part 5, pp. 1040 - 1044

Phylogenetic tree based on almost-complete 16S rRNA gene sequences and reconstructed using the program DNAPARS implemented in the PHYLIP package of the ARB software (version 03_08_22) showing the position of strain DS . [PDF](379 KB)

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