1887

Abstract

Two strictly anaerobic, crescent-shaped bacterial strains, designated WG and Ycb08, were isolated from a cellulose-degrading mixed culture enriched from yak rumen contents. The strains were Gram-negative, non-spore-forming and motile, with four to six flagella situated at the centre of the concave side of the cell. The cells were 0.9–1.1×4–6 μm. Growth was observed at 27–46 °C (optimum 39 °C) and pH 4.2–8.3 (optimum pH 7.0–7.2). Arabinose, glucose, mannose, cellobiose, lactose, sucrose, trehalose, melibiose, raffinose, salicin and aesculin were fermented. The end products of glucose fermentation were acetate, propionate and CO. The G+C contents of strains WG and Ycb08 were respectively 63.9±0.2 and 62.5±0.2 mol% ( ). Phylogenetic analysis based on 16S rRNA gene sequences revealed that the two strains were related to the genera and at similarity levels below 97 %; however, they differed from members of the genus in their flagellar arrangement. On the basis of phenotypic, genotypic and physiological evidence, strains WG and Ycb08 are identified as members of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is WG (=CGMCC 1.5073 =JCM 15470).

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2009-08-01
2024-03-19
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vol. , part 8, pp. 2080 - 2083

Transmission electron micrograph of negatively stained cell of sp. nov. WG . The tuft of flagella is shown. Bar, 1 µm.



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