RT Journal Article SR Electronic(1) A1 Guo, Xu A1 You, Xiao-Yan A1 Liu, Li-Jun A1 Zhang, Jia-Yue A1 Liu, Shuang-Jiang A1 Jiang, Cheng-YingYR 2009 T1 Alicyclobacillus aeris sp. nov., a novel ferrous- and sulfur-oxidizing bacterium isolated from a copper mine JF International Journal of Systematic and Evolutionary Microbiology, VO 59 IS 10 SP 2415 OP 2420 DO https://doi.org/10.1099/ijs.0.008870-0 PB Microbiology Society, SN 1466-5034, AB A novel mesophilic, acidophilic, endospore-forming bacterium, designated strain ZJ-6T, was isolated from Zi-Jin copper mine in Inner Mongolia, China. Cells of strain ZJ-6T were rod-shaped, stained Gram-positive or were Gram-variable, and grew aerobically at 25–35 °C (optimum, 30 °C) and pH 2.0–6.0 (optimum, pH 3.5). 16S rRNA gene sequence analysis showed that strain ZJ-6T was related phylogenetically to members of the genus Alicyclobacillus, with 16S rRNA gene sequence similarities of 89.5–94.2 %. Cells contained MK-7 as the major quinone and the DNA G+C content was 51.2 mol%. Strain ZJ-6T possessed a number of phenotypic characteristics that differentiated it from recognized Alicyclobacillus species, including its growth temperature, assimilation of various carbon sources, production of acids from a range of compounds, and the ability to grow chemoautotrophically using ferrous iron, elemental sulfur and tetrathionate as electron donors. The predominant cellular fatty acids of strain ZJ-6T were anteiso-C15 : 0 (67.1 %), iso-C16 : 0 (7.7 %) and anteiso-C17 : 0 (7.4 %); ω-alicyclic fatty acids were not found. On the basis of these results, it is concluded that strain ZJ-6T represents a novel species within the genus Alicyclobacillus, for which the name Alicyclobacillus aeris sp. nov. is proposed; the type strain is ZJ-6T (=CGMCC 1.7072T=NBRC 104953T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.008870-0