RT Journal Article SR Electronic(1) A1 Zhao, Xin-Qing A1 Li, Wen-Jun A1 Jiao, Wen-Ce A1 Li, Yan A1 Yuan, Wen-Jie A1 Zhang, Yu-Qin A1 Klenk, Hans-Peter A1 Suh, Joo-Won A1 Bai, Feng-WuYR 2009 T1 Streptomyces xinghaiensis sp. nov., isolated from marine sediment JF International Journal of Systematic and Evolutionary Microbiology, VO 59 IS 11 SP 2870 OP 2874 DO https://doi.org/10.1099/ijs.0.009878-0 PB Microbiology Society, SN 1466-5034, AB A novel actinomycete, strain S187T, was isolated from a marine sediment sample collected from Xinghai Bay, Dalian, China. Growth occurred on ISP medium 2 containing 0–9 % NaCl and at pH 6.0–9.0 and 10–45 °C. The cell wall of strain S187T contained the isomer ll-diaminopimelic acid as the diagnostic diamino acid. The predominant menaquinones were MK-9(H6) (40.8 %), MK-9(H8) (38.2 %) and MK-9(H2) (8.8 %). The major fatty acids were iso-C16 : 0 (29.6 %), anteiso-C15 : 0 (14.0 %) and anteiso-C17 : 0 (11.6 %). Cells contained phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol, phosphatidylinositol mannosides and one unknown phospholipid. The G+C content of the genomic DNA was 72.01 mol%. The 16S rRNA gene sequence of the isolate had similarities of 98.1 and 97.5 % with those of Streptomyces flavofuscus NRRL B-8036T (=DSM 41426T) and Streptomyces albiaxialis DSM 41799T, respectively, showing that the novel strain should be assigned to the genus Streptomyces. DNA–DNA hybridizations with the two above-mentioned Streptomyces species showed 31.4 and 46.9 % relatedness, respectively. Moreover, the three strains differed in some physiological and biochemical properties. Thus, on the basis of phenotypic and genotypic analyses, it is proposed that strain S187T represents a novel species of the genus Streptomyces, for which the name Streptomyces xinghaiensis sp. nov. is proposed; the type strain is S187T (=NRRL B-24674T=CCTCC AA 208049T=KCTC 19546T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.009878-0