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f gen. nov., sp. nov., a reductively dehalogenating bacterium isolated from chlorinated solvent-contaminated groundwater

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  • Authors: William M. Moe1 ,  Jun Yan1 ,  M. Fernanda Nobre2 ,  Milton S. da Costa3 , Fred A. Rainey4
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    • 1 1Department of Civil and Environmental Engineering, Louisiana State University, Baton Rouge, LA 70803, USA 2 2Departamento de Zoologia and Centro de Neurociências, Universidade de Coimbra, 3004-517 Coimbra, Portugal 3 3Departamento de Bioquímica and Centro de Neurociências, Universidade de Coimbra, 3001-401 Coimbra, Portugal 4 4Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA
  • William M. Moe
 [email protected]
  • First Published Online: 01 November 2009, International Journal of Systematic and Evolutionary Microbiology 59: 2692-2697, doi: 10.1099/ijs.0.011502-0
  • Subject: New Taxa - Other Bacteria
  • Cover date:
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gen. nov., sp. nov., a reductively dehalogenating bacterium isolated from chlorinated solvent-contaminated groundwater, Page 1 of 1

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  • Two recently reported bacterial strains that are able to reductively dehalogenate polychlorinated aliphatic alkanes, including 1,2,3-trichloropropane, 1,2-dichloropropane, 1,1,2,2-tetrachloroethane, 1,1,2-trichloroethane and 1,2-dichloroethane, were further characterized to clarify their taxonomic position. The two strains, designated BL-DC-8 and BL-DC-9, were mesophilic, non-spore-forming, non-motile, Gram-negative staining and strictly anaerobic. Cells were irregular cocci, 0.3–0.6 μm in diameter. The two strains were resistant to ampicillin and vancomycin. Hydrogen was utilized as an electron donor. The genomic DNA G+C content of strains BL-DC-8 and BL-DC-9 was 54.0 and 53.8 mol%, respectively. The major cellular fatty acids were C 9, C 9, C and C. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the strains cluster within the phylum , but are related only distantly to all recognized taxa in the phylum. Morphological, physiological and chemotaxonomic traits as well as phylogenetic analysis support the conclusion that these two strains represent a novel species of a new genus in the phylum , for which the name gen. nov., sp. nov. is proposed. The type strain of is BL-DC-9 (=ATCC BAA-1523 =JCM 15061).

  • The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains BL-DC-9 and BL-DC-8 are EU679419 and EU679418, respectively.

  • Scanning electron and transmission electron micrographs of cells of strains BL-DC-9 and BL-DC-8 and phenotypic characteristics of type species of genera in the phylum are available with the online version of this paper.

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  • Adrian, L., Szewzyk, U., Wecke, J. & Gorisch, H. ( 2000; ). Bacterial dehalorespiration with chlorinated benzenes. Nature 408, 580–583.[CrossRef]
    [Google Scholar]
  • Bowman, K. S., Moe, W. M., Rash, B. A., Bae, H. S. & Rainey, F. A. ( 2006; ). Bacterial diversity of an acidic Louisiana groundwater contaminated by dense nonaqueous-phase liquid containing chloroethanes and other solvents. FEMS Microbiol Ecol 58, 120–133.[CrossRef]
    [Google Scholar]
  • Castenholz, R. W. ( 2001; ). Class I. “Chloroflexi”. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1, p. 427. Edited by D. R. Boone, R. W. Castenholz & G. M. Garrity. New York: Springer.
  • Demharter, W., Hensel, R., Smida, J. & Stackebrandt, E. ( 1989; ). Sphaerobacter thermophilus gen. nov., sp. nov. A deeply rooting member of the actinomycetes subdivision isolated from thermophilically treated sewage sludge. Syst Appl Microbiol 11, 261–266.[CrossRef]
    [Google Scholar]
  • Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  • Hanada, S., Hiraishi, A., Shimada, K. & Matsuura, K. ( 1995; ). Chloroflexus aggregans sp. nov., a filamentous phototrophic bacterium which forms dense cell aggregates. Int J Syst Bacteriol 45, 676–681.[CrossRef]
    [Google Scholar]
  • Hanada, S., Takaichi, S., Matsuura, K. & Nakamura, K. ( 2002; ). Roseiflexus castenholzii gen. nov., sp. nov., a thermophilic, filamentous, photosynthetic bacterium that lacks chlorosomes. Int J Syst Evol Microbiol 52, 187–193.
     [Google Scholar]
  • He, J., Ritalahti, K. M., Yang, K.-L., Koenigsbeg, S. S. & Löffler, F. E. ( 2003; ). Detoxification of vinyl chloride to ethene coupled to growth of an anaerobic bacterium. Nature 424, 62–64.[CrossRef]
    [Google Scholar]
  • He, J., Sung, Y., Krajmalnik-Brown, R., Ritalahti, K. M. & Löffler, F. E. ( 2005; ). Isolation and characterization of Dehalococcoides sp. strain FL2, a trichloroethene (TCE)- and 1,2-dichloroethene-respiring anaerobe. Environ Microbiol 7, 1442–1450.[CrossRef]
    [Google Scholar]
  • Hendrickson, E. R., Payne, J. A., Young, R. M., Starr, M. G., Perry, M. P., Fahnestock, S., Ellis, D. E. & Ebersole, R. C. ( 2002; ). Molecular analysis of Dehalococcoides 16S ribosomal DNA from chloroethene-contaminated sites throughout North America and Europe. Appl Environ Microbiol 68, 485–495.[CrossRef]
    [Google Scholar]
  • Holt, J. G. & Castenholz, R. W. ( 2001; ). Genus I. Herpetosiphon Holt and Lewin 1968, 2408AL. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1, pp. 445–446. Edited by D. R. Boone, R. W. Castenholz & G. M. Garrity. New York: Springer.
  • Holt, J. G. & Lewin, R. A. ( 1968; ). Herpetosiphon aurantiacus gen. et sp. n., a new filamentous gliding organism. J Bacteriol 95, 2407–2408.
     [Google Scholar]
  • Hugenholtz, P. & Stackebrandt, E. ( 2004; ). Reclassification of Sphaerobacter thermophilus from the subclass Sphaerobacteridae in the phylum Actinobacteria to the class Thermomicrobia (emended description) in the phylum Chloroflexi (emended description). Int J Syst Evol Microbiol 54, 2049–2051.[CrossRef]
    [Google Scholar]
  • Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  • Kube, M., Beck, A., Zinder, S. H., Kuhl, H., Reinhardt, R. & Adrian, L. ( 2005; ). Genome sequence of the chlorinated compound-respiring bacterium Dehalococcoides species strain CBDB1. Nat Biotechnol 23, 1269–1273.[CrossRef]
    [Google Scholar]
  • Laakso, I., Seppänen-Laakso, T., Hiltunen, R., Müller, B., Jansen, H. & Knobloch, K. ( 1989; ). Volatile garlic odor components: gas phases and adsorbed exhaled air analyzed by headspace gas chromatography-mass spectrometry. Planta Med 55, 257–261.[CrossRef]
    [Google Scholar]
  • Lapage, S. P., Sneath, P. H. A., Lessel, E. F., Skerman, V. B. D., Seeliger, H. P. R. & Clark, W. A. (editors) ( 1992; ). International Code of Nomenclature of Bacteria (1990 Revision). Bacteriological Code. Washington, DC: American Society for Microbiology.
  • Lawson, L. D. ( 1996; ). The composition and chemistry of garlic cloves and processed garlic. In Garlic: The Science and Therapeutic Application of Allium sativum L. and Related Species, 2nd edn, pp. 37–107. Edited by H. P. Kock & L. D. Lawson. Baltimore: Williams & Wilkins.
  • Lewin, R. A. ( 1970; ). New Herpetosiphon species (Flexibacterales). Can J Microbiol 16, 517–520.[CrossRef]
    [Google Scholar]
  • Ludwig, W., Strunk, O., Westram, R., Richter, L., Meier, H., Yadhukumar, Buchner, A., Lai, T., Steppi, S. & other authors ( 2004; ). arb: a software environment for sequence data. Nucleic Acids Res 32, 1363–1371.[CrossRef]
    [Google Scholar]
  • Maymó-Gatell, X., Chien, Y., Gossett, J. M. & Zinder, S. H. ( 1997; ). Isolation of a bacterium that reductively dechlorinates tetrachloroethene to ethene. Science 276, 1568–1571.[CrossRef]
    [Google Scholar]
  • Maymó-Gatell, X., Anguish, T. & Zinder, S. H. ( 1999; ). Reductive dechlorination of chlorinated ethenes and 1,2-dichloroethane by ‘Dehalococcoides ethenogenes’ 195. Appl Environ Microbiol 65, 3108–3113.
     [Google Scholar]
  • Maymó-Gatell, X., Nijenhuis, I. & Zinder, S. H. ( 2001; ). Reductive dechlorination of cis-1,2-dichloroethene and vinyl chloride by ‘Dehalococcoides ethenogenes’. Environ Sci Technol 35, 516–521.[CrossRef]
    [Google Scholar]
  • Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  • Perry, J. J. ( 2001; ). Genus I. Thermomicrobium Jackson, Ramaley and Meinschein 1973, 34AL. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1, pp. 448–450. Edited by D. R. Boone, R. W. Castenholz & G. M. Garrity. New York: Springer.
  • Pierson, B. K. & Castenholz, R. W. ( 1974; ). A phototrophic gliding filamentous bacterium of hot springs, Chloroflexus aurantiacus, gen. and sp. nov. Arch Microbiol 100, 5–24.[CrossRef]
    [Google Scholar]
  • Pierson, B. K., Giovannoni, S. J., Stahl, D. A. & Castenholz, R. W. ( 1985; ). Heliothrix oregonensis, gen. nov., sp. nov., a phototrophic filamentous gliding bacterium containing bacteriochlorophyll a. Arch Microbiol 142, 164–167.[CrossRef]
    [Google Scholar]
  • Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  • Sekiguchi, Y., Yamada, T., Hanada, S., Ohashi, A., Harada, H. & Kamagata, Y. ( 2003; ). Anaerolinea thermophila gen. nov., sp. nov. and Caldilinea aerophila gen. nov., sp. nov., novel filamentous thermophiles that represent a previously uncultured lineage of the domain Bacteria at the subphylum level. Int J Syst Evol Microbiol 53, 1843–1851.[CrossRef]
    [Google Scholar]
  • Seshadri, R., Adrian, L., Fouts, D. E., Eisen, J. A., Phillippy, A. M., Methe, B. A., Ward, N. L., Nelson, W. C., Deboy, R. T. & other authors ( 2005; ). Genome sequence of the PCE-dechlorinating bacterium Dehalococcoides ethenogenes. Science 307, 105–108.[CrossRef]
    [Google Scholar]
  • Sung, Y., Ritalahti, K. M., Apkarian, R. P. & Löffler, F. E. ( 2006; ). Quantitative PCR confirms purity of strain GT, a novel trichloroethene-to-ethene-respiring Dehalococcoides isolate. Appl Environ Microbiol 72, 1980–1987.[CrossRef]
    [Google Scholar]
  • White, D. C., Geyer, R., Peacock, A. D., Hedrick, D. B., Koenigsberg, S. S., Sung, Y., He, J. & Löffler, F. E. ( 2005; ). Phospholipid furan fatty acids and ubiquinone-8: lipid biomarkers that may protect Dehalococcoides strains from free radicals. Appl Environ Microbiol 71, 8426–8433.[CrossRef]
    [Google Scholar]
  • Yamada, T., Sekiguchi, Y., Hanada, S., Imachi, H., Ohashi, A., Harada, H. & Kamagata, Y. ( 2006; ). Anaerolinea thermolimosa sp. nov., Levilinea saccarolytica gen. nov., sp. nov. and Leptolinea tardivitalis gen. nov., sp. nov., novel filamentous anaerobes, and description of the new classes Anaerolineae classis nov. and Caldineae classis nov. in the bacterial phylum Chloroflexi. Int J Syst Evol Microbiol 56, 1331–1340.[CrossRef]
    [Google Scholar]
  • Yamada, T., Imachi, H., Ohashi, A., Harada, H., Hanada, S., Kamagata, Y. & Sekiguchi, Y. ( 2007; ). Bellilinea caldifistulae gen. nov., sp. nov. and Longilinea arvoryzae gen. nov., sp. nov., strictly anaerobic, filamentous bacteria of the phylum Chloroflexi isolated from methanogenic propionate-degrading consortia. Int J Syst Evol Microbiol 57, 2299–2306.[CrossRef]
    [Google Scholar]
  • Yan, J., Rash, B. A., Rainey, F. A. & Moe, W. M. ( 2009; ). Isolation of novel bacteria within the Chloroflexi capable of reductive dechlorination of 1,2,3-trichloropropane. Environ Microbiol 11, 833–843.[CrossRef]
    [Google Scholar]
  • Zehnder, A. J. B. & Wuhrmann, K. ( 1976; ). Titanium (III) citrate as a nontoxic oxidation–reduction buffering system for the culture of obligate anaerobes. Science 194, 1165–1166.[CrossRef]
    [Google Scholar]
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