RT Journal Article SR Electronic(1) A1 Kitahara, Maki A1 Sakamoto, Mitsuo A1 Benno, YoshimiYR 2010 T1 Lactobacillus similis sp. nov., isolated from fermented cane molasses JF International Journal of Systematic and Evolutionary Microbiology, VO 60 IS 1 SP 187 OP 190 DO https://doi.org/10.1099/ijs.0.011551-0 PB Microbiology Society, SN 1466-5034, AB The taxonomic position of strain JCM 2765T isolated from fermented cane molasses in Thailand was reinvestigated. Strain JCM 2765T was originally identified as representing Lactobacillus buchneri on the basis of biochemical and physiological characteristics. In the present study, 16S rRNA gene sequence analysis of strain JCM 2765T demonstrated a low level of similarity with the type strain of L. buchneri (92.5 %) and high levels with those of Lactobacillus collinoides (97.6 %) and Lactobacillus paracollinoides (98.0 %). Ribotyping was applied to investigate the relationships between strain JCM 2765T, L. collinoides and L. paracollinoides. The dendrogram based on ribotyping patterns showed one cluster for six strains of L. paracollinoides, and that strain JCM 2765T and L. collinoides JCM 1123T were each independent. Based on additional phenotypic findings and DNA–DNA hybridization results, strain JCM 2765T is considered to represent a novel species of the genus Lactobacillus, for which the name Lactobacillus similis sp. nov. is proposed. The type strain is JCM 2765T (=LMG 23904T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.011551-0